Structural and ultrastructural studies of male reproductive tract and spermatozoa in <i>Xylocopa frontalis</i> (Hymenoptera, Apidae)

Fiorillo, Bruno Silva; Zama, Uyrá; Lino‐Neto, José; Báo, Sônia Nair

doi:10.1111/j.1463-6395.2009.00396.x

Cited by 9 publications

(19 citation statements)

References 24 publications

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“…Morphological aspects of the male reproductive system of parasitoids analyzed are similar to those described for most Hymenoptera (Dallacqua and Cruz-Landim, 2003;Ferreira et al, 2004;Damiens and Boivin, 2005;Araú jo et al, 2005a;Brito et al, 2005;Bushrow et al, 2006;Fiorillo et al, 2008Fiorillo et al, , 2009). However, the number of follicles per testis varies considerably between the different groups.…”

Section: Discussionmentioning

confidence: 90%

“…All studied species of Hymenoptera which have been morphological and which had their reproductive system characterized showed a dilated and modified region in the deferent ducts specialized for the storage of spermatozoa-a seminal vesicle (Dallacqua and Cruz-Landim, 2003;Ferreira et al, 2004;Damiens and Boivin, 2005;Araú jo et al, 2005a;Brito et al, 2005;Bushrow et al, 2006;Lima et al, 2006;Fiorillo et al, 2009). In some Chalcidoidea it is formed of two distinct morphological portions (Gerling and Legner, 1968;Damiens and Boivin, 2005;Fiorillo et al, 2008).…”

Section: Discussionmentioning

confidence: 96%

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Structural and ultrastructural characteristics of male reproductive tract and spermatozoa in two Cryptinae species (Hymenoptera: Ichneumonidae)

Moreira

Araújo

Báo

et al. 2010

Micron

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This study represents the first characterization of male reproductive tracts and ultrastructural description of sperm of Cryptinae species. In Lymeon dieloceri and Pachysomoides sp., the male reproductive tract is formed by a pair of testis, two deferent ducts, two accessory glands and one ejaculatory duct. The spermatozoa are similar to those described for other Hymenoptera, with: (1) the acrosome formed by the acrosomal vesicle covering the perforatorium, which has its base inserted in a cavity located in the nucleus point; (2) a thin nucleus with electron-dense chromatin; (3) an electron-dense centriolar adjunct located between the nucleus and one of the two mitochondrial derivatives; (4) an axoneme with a 9 + 9 + 2 microtubule arrangement; (5) two long mitochondrial derivatives with peripheral cristae and; (6) two accessory bodies located between the two mitochondrial derivatives and the axoneme. These ichneumonids present structural characteristics similar to other parasitic wasps, such as presence of a single follicle per testis, layer of extracellular material enveloping the acrosome and accessory microtubules ending before others in final portion of the flagellum. However, male reproductive system and the spermatozoa presented morphological characteristics that allowed their differentiation, such as oval shapes accessory glands and the symmetric mitochondrial derivatives in L. dieloceri compared to the spherical accessory glands and asymmetrical derivatives observed in Pachysomoides sp. Taken together, data presented here demonstrates that diversity of morphological characteristic from the male reproductive tract and spermatozoa in Hymenoptera might provide a character system that can be used, in association with other systems, to resolve various uncertainties about the evolutionary relationships of this insect group.

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Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 96%

Structural and ultrastructural characteristics of male reproductive tract and spermatozoa in two Cryptinae species (Hymenoptera: Ichneumonidae)

Moreira

Araújo

Báo

et al. 2010

Micron

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“…In Scelionidae, only one mitochondrial derivative was observed, extending to the anterior extremity of the nucleus (Lino-Neto and Dolder, 2001a). The termination of the mitochondrial derivatives in the final portion of the flagellum in D. connubialis is similar to that observed in most Aculeata Apidae (Zama et al, 2001(Zama et al, , 2004(Zama et al, , 2005aFiorillo et al, 2005Fiorillo et al, , 2009Gracielle et al, 2009), Sphecidae (Zama et al, 2005b) and Vespidae (Mancini et al, 2006(Mancini et al, , 2009, in which one mitochondrial derivative terminates before the other, but differs from P. nasuta, in which the two mitochondrial derivatives terminate together (Oliveira et al, 2010).…”

Section: Discussionmentioning

confidence: 64%

Morphology of spermatozoa of Dissomphalus connubialis (Ashmead, 1893) (Hymenoptera: Bethylidae)

Oliveira

Fraga

Brito

et al. 2013

Micron

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In this study, the morphology of spermatozoa of Bethylidae Dissomphalus connubialis (Pristocerinae) was analyzed using light and transmission electron microscopy. Spermatozoa of this species are thin, measure approximately 130 μm in length and comprise a head region and flagellum region. The head is formed by the acrosome and nucleus. The acrosome consists of the acrosome vesicle and the perforatorium, the posterior portion of which is inserted into a cavity at the anterior extremity of the nucleus. The nucleus is compact, electron-dense and measures 15 μm in length. The flagellum possesses two mitochondrial derivatives, two accessory bodies and one axoneme with a 9+9+2 microtubular pattern. The nucleus is connected to the flagellum by the centriole adjunct. Mitochondrial derivatives are compact, apparently without paracrystalline material and with rare mitochondrial cristae. They are asymmetric in length, such that the larger mitochondrial derivative begins parallel to the posterior region of the nucleus and the smaller mitochondrial derivative begins just below the centriole adjunct. The basic structure of spermatozoa of D. connubialis is similar to that of other Aculeata studied. However, this species shows characteristics not seen in other Hymenoptera, such as the wide electron-lucid region that separates the acrosomal vesicle from the perforatorium and the depth of the cavity in the anterior extremity of the nucleus, into which the base of the perforatorium is inserted. There are also characteristics that distinguish this species from Bethylidae Prorops nasuta, including the fact that one of the mitochondrial derivatives lies to parallel to the nucleus over a long distance, the small quantity of cristae, the absence of paracrystalline material in these organelles, and the fact that the accessory microtubules are the first to terminate in the final portion of the flagellum.

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“…Many similar morphological characteristics associated with the male reproductive system were found in comparative studies on the Hymenoptera (Araú jo et al., 2005a,b, 2009Brito et al, 2005;Bushrow et al, 2006;Dallacqua and Cruz-Landim, 2003;Damiens and Boivin, 2005;Ferreira et al, 2004;Fiorillo et al, 2008Fiorillo et al, , 2009Moreira et al, 2008Moreira et al, , 2010. On the other hand, these studies also identified several features that differed between the various groups.…”

Section: Discussionmentioning

confidence: 79%

Morphological aspects of testes and sperm ultrastructure in the “symphyta” Digelasinus diversipes kirby 1882 (hymenoptera: Argidae: Dielocerinae)

Moreira

Araújo

Lino‐Neto

2011

Microscopy Res & Technique

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In Digelasinus diversipes, spermatozoa are maintained in bundles, with 74 spermatozoa on average, in the seminal vesicle. These spermatozoa are very short (20 μm) and consist of a head and flagellum. The head includes an acrosome (perforatorium covered by the acrosomal vesicle) and a nucleus. A regular electron-lucent region separates the acrosomal vesicle from the perforatorium, which is inserted parallel to the anterior ending of the nucleus. The small flagellum is composed of two symmetrical mitochondrial derivatives, a centriolar adjunct, an axoneme (9 + 9 + 2), and two accessory bodies. The centriolar adjunct begins above the posterior end of the nucleus and ends covering the anterior tip of two mitochondrial derivatives. In the terminal region of the axoneme, the central microtubules terminate first. The presence of a subacrosomal space, a short mitochondrial derivative diameter, and a short spermatodesm is the ultrastructure characteristics of spermatozoa shared by all "symphyta" species. Differences in the insertion of the perforatorium into the nucleus and the position of the centriolar adjunct distinguish Dielocerinae and the Arginae studied previously. The number of spermatozoa per cyst is variable. Furthermore, additional characteristics that had not been described for "symphyta" were also found, such as the number of follicles per testis.

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Structural and ultrastructural studies of male reproductive tract and spermatozoa in Xylocopa frontalis (Hymenoptera, Apidae)

Cited by 9 publications

References 24 publications

Structural and ultrastructural characteristics of male reproductive tract and spermatozoa in two Cryptinae species (Hymenoptera: Ichneumonidae)

Structural and ultrastructural characteristics of male reproductive tract and spermatozoa in two Cryptinae species (Hymenoptera: Ichneumonidae)

Morphology of spermatozoa of Dissomphalus connubialis (Ashmead, 1893) (Hymenoptera: Bethylidae)

Morphological aspects of testes and sperm ultrastructure in the “symphyta” Digelasinus diversipes kirby 1882 (hymenoptera: Argidae: Dielocerinae)

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