Structural Aspects and Prediction of Calmodulin-Binding Proteins

Andrews, Corey; Xu, Yiting; Kirberger, Michael

doi:10.3390/ijms22010308

Cited by 42 publications

(26 citation statements)

References 92 publications

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“…Diverse Calcium-Mediated Signaling Pathways Are Induced in the Cold-Tolerant rhododendron chrysanthum It is becoming clear that Ca2 + signaling plays a crucial role in conferring cold tolerance in plants [7]. In addition, the calcium would bind to the calmodulin [30] to phosphorylate calcium/CaM-binding protein (CaM-BP) to regulate gene expression [31]. In this study, the cold stress-phosphorylated CaM-binding protein implied their possible involvement in calcium/CaM signaling events (Fig.…”

Section: Diverse Ros Scavenging Pathways Are Employed For Cold Stress Responsesmentioning

confidence: 99%

Phosphoproteomics of Cold Stress-Responsive Mechanisms in Rhododendron Chrysanthum

liu

Zhang

Fan

et al. 2021

Preprint

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Background: As an alpine plants，Rhododendron chrysanthum (R. chrysanthum) has evolved cold resistance mechanisms and become a valuable plant resource with the responsive mechanism of cold stress. Results: In my study, we adopt the phosphoproteomic and proteomic analysis combining with physiological measurement to illustrate the responsive mechanism of R. chrysanthum seedling under cold (4℃) stress. After chilling for 12 h, 350 significantly changed proteins and 274 significantly changed phosphoproteins were detected. COG analysis showed that significantly changed proteins and phosphoproteins were mainly involved in signal transduction and energy production and conversion under cold stress. The results indicated photosynthesis was inhibited under cold stress, but cold induced calcium-mediated signaling, reactive oxygen species (ROS) homeostasis and other transcription regulation factors could protect plants from destruction caused by cold stress. Conclusions: These data constitute a cold stress-responsive metabolic atlas in R. chrysanthum, which will springboard further investigations into the complex molecular mechanisms of plant cold adaptation.

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Section: Diverse Ros Scavenging Pathways Are Employed For Cold Stress Responsesmentioning

confidence: 99%

Phosphoproteomics of Cold Stress-Responsive Mechanisms in Rhododendron Chrysanthum

liu

Zhang

Fan

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…Calcium-mediated signaling pathways are induced in the cold-tolerant rhododendron chrysanthum It is becoming clear that Ca2+ signaling plays a crucial role in conferring cold tolerance in plants [7]. In addition, the calcium would bind to the calmodulin [30] to phosphorylate calcium/CaM-binding protein (CaM-BP) to regulate gene expression [31]. In this study, the cold stress-phosphorylated CaM-binding protein implied their possible involvement in calcium/CaM signaling events (Figure 6, Supplementary Table S1 and Supplementary Table S2).…”

Section: Ros-scavenging Pathways Of Rhododendron Chrysanthum Are Employed For Cold Stress Responsesmentioning

confidence: 99%

Phosphoproteomics of Cold Stress-Responsive Mechanisms in Rhododendron Chrysanthum

Liu

Zhang

Fan

et al. 2021

Preprint

View full text Add to dashboard Cite

As an alpine plant，Rhododendron chrysanthum (R. chrysanthum) has evolved cold resistance mechanisms and become a valuable plant resource with the responsive mechanism of cold stress. In my study, we adopt the phosphoproteomic and proteomic analysis combining with physiological measurement to illustrate the responsive mechanism of R. chrysanthum seedling under cold (4℃) stress. After chilling for 12 h, 350 significantly changed proteins and 274 significantly changed phosphoproteins were detected. Clusters of Orthologous Groups（COG）analysis showed that significantly changed proteins and phosphoproteins were mainly involved in signal transduction and energy production and conversion under cold stress. The results indicated photosynthesis was inhibited under cold stress, but cold induced calcium-mediated signaling, reactive oxygen species (ROS) homeostasis and other transcription regulation factors could protect plants from the destruction caused by cold stress. These results provide a detailed insight into the cold stress response and defense mechanisms of R. chrysanthum leaves at the phosphoproteome level.

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“…Although improved surgical techniques and neoadjuvant chemotherapy are available, the 5-year overall survival of OS is just 60–70% [ 2 ]. About 20% of OS patients already have lung metastases when they are diagnosed, with the 5-year overall survival rate of < 30% [ 3 ]. Consequently, elucidating the OS metastatic mechanism is of great importance.…”

Section: Introductionmentioning

confidence: 99%

Exosomal transfer of miR-769-5p promotes osteosarcoma proliferation and metastasis by targeting DUSP16

et al. 2021

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Background Osteosarcoma (OS) is a malignant tumor originating from mesenchymal stem cells, and has an extremely high fatality rate and ability to metastasize. Although mounting evidence suggests that miR-769-5p is strongly associated with the malignant progression and poor prognosis of various tumors, the exact role of miR-769-5p in OS is still unclear. Therefore, this study aimed to explore the relationship between miR-769-5p and the malignant progression of OS, and its underlying mechanism of action. Methods miR-769-5p expression was analyzed in GSE28423 from the GEO database and measured in OS clinical specimens and cell lines. The effects of miR-769-5p on OS proliferation, migration and invasion were measured both in vivo and in vitro. In addition, bioinformatics analyses and luciferase reporter assays were used to explore the target genes of miR-769-5p. Rescue experiments were also conducted. Moreover, a co-culture model was used to test the cell interaction between bone mesenchymal stem cells (BMSC) and OS cells. Results We found that miR-769-5p is highly expressed in OS clinical specimens and cell lines. In vivo and in vitro experiments also showed that miR-769-5p significantly promoted the proliferation, migration and invasion of OS cells. Dual-specific phosphatase 16 (DUSP16) was negatively associated with miR-769-5p expression in OS cells and tissue samples and was validated as the downstream target by luciferase reporter assay and western blotting. Rescue experiments showed that DUSP16 reverses the effect of miR-769-5p on OS cells by negatively regulating the JNK/p38 MAPK signaling pathway. Additionally, the results of the co-culture of BMSCs and OS cells confirmed that miR-769-5p was transferred from BMSCs to OS cells through exosomes. Conclusions In summary, this study demonstrates for the first time that BMSC-derived exosomal miR-769-5p promotes OS proliferation and metastasis by targeting DUSP16 and activating the JNK/p38 MAPK signaling pathway, which could provide rationale for a new therapeutic strategy for OS.

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Structural Aspects and Prediction of Calmodulin-Binding Proteins

Cited by 42 publications

References 92 publications

Phosphoproteomics of Cold Stress-Responsive Mechanisms in Rhododendron Chrysanthum

Phosphoproteomics of Cold Stress-Responsive Mechanisms in Rhododendron Chrysanthum

Phosphoproteomics of Cold Stress-Responsive Mechanisms in Rhododendron Chrysanthum

Exosomal transfer of miR-769-5p promotes osteosarcoma proliferation and metastasis by targeting DUSP16

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