Structural basis for inhibition of an archaeal CRISPR–Cas type I-D large subunit by an anti-CRISPR protein

Manav, M.C.; Van, Lan B.; Lin, Jinzhong; Fuglsang, Anders; Peng, Xu; Brodersen, D.E.

doi:10.1038/s41467-020-19847-x

Cited by 22 publications

(25 citation statements)

References 55 publications

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“…Notably, AcrIIA6 and AcrVA4 also harbor an HTH domain and induces the dimerization of Cas9 from Streptococcus thermophilus and Cas12a from Lachnospiraceae bacterium , respectively 29 , 33 , 34 . Recently, the AcrID1 dimer was reported to sequester the Cas10d large subunit of type I-D system to form a 4:2 complex 35 . Therefore, AcrIF24 also represents an Acr which induces the dimerization of the Cascade complex in class 1 CRISPR-Cas systems and a verified class 1 Acr with Aca-like functions.…”

Section: Discussionmentioning

confidence: 99%

Insights into the inhibition of type I-F CRISPR-Cas system by a multifunctional anti-CRISPR protein AcrIF24

et al. 2022

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CRISPR-Cas systems are prokaryotic adaptive immune systems and phages use anti-CRISPR proteins (Acrs) to counteract these systems. Here, we report the structures of AcrIF24 and its complex with the crRNA-guided surveillance (Csy) complex. The HTH motif of AcrIF24 can bind the Acr promoter region and repress its transcription, suggesting its role as an Aca gene in self-regulation. AcrIF24 forms a homodimer and further induces dimerization of the Csy complex. Apart from blocking the hybridization of target DNA to the crRNA, AcrIF24 also induces the binding of non-sequence-specific dsDNA to the Csy complex, similar to AcrIF9, although this binding seems to play a minor role in AcrIF24 inhibitory capacity. Further structural and biochemical studies of the Csy-AcrIF24-dsDNA complexes and of AcrIF24 mutants reveal that the HTH motif of AcrIF24 and the PAM recognition loop of the Csy complex are structural elements essential for this non-specific dsDNA binding. Moreover, AcrIF24 and AcrIF9 display distinct characteristics in inducing non-specific DNA binding. Together, our findings highlight a multifunctional Acr and suggest potential wide distribution of Acr-induced non-specific DNA binding.

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Section: Discussionmentioning

confidence: 99%

Insights into the inhibition of type I-F CRISPR-Cas system by a multifunctional anti-CRISPR protein AcrIF24

et al. 2022

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“…The type I-D operon contains the type I signature gene cas3 split into its two domains; the helicase domain (Cas3′) encoded from a stand-alone gene, and the HD nuclease domain (Cas3″), which is fused to the large subunit as part of the core effector complex. The Cas10d subunit is bigger than other type I large subunits (Cas8) and similar to type III large subunits 24 , 25 , 28 , yet retains the type I functionality of discerning PAMs in target DNA sequences 29 , 30 .…”

Section: Introductionmentioning

confidence: 99%

Structural rearrangements allow nucleic acid discrimination by type I-D Cascade

et al. 2022

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CRISPR-Cas systems are adaptive immune systems that protect prokaryotes from foreign nucleic acids, such as bacteriophages. Two of the most prevalent CRISPR-Cas systems include type I and type III. Interestingly, the type I-D interference proteins contain characteristic features of both type I and type III systems. Here, we present the structures of type I-D Cascade bound to both a double-stranded (ds)DNA and a single-stranded (ss)RNA target at 2.9 and 3.1 Å, respectively. We show that type I-D Cascade is capable of specifically binding ssRNA and reveal how PAM recognition of dsDNA targets initiates long-range structural rearrangements that likely primes Cas10d for Cas3′ binding and subsequent non-target strand DNA cleavage. These structures allow us to model how binding of the anti-CRISPR protein AcrID1 likely blocks target dsDNA binding via competitive inhibition of the DNA substrate engagement with the Cas10d active site. This work elucidates the unique mechanisms used by type I-D Cascade for discrimination of single-stranded and double stranded targets. Thus, our data supports a model for the hybrid nature of this complex with features of type III and type I systems.

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“…Cryo‐electron microscopy has flourished recent years, these technological breakthroughs make structural biology entered a new era. [ 71–76 ] We suppose that the cryo‐electron microscopy is likely to be used to unravel the mechanism underlying optical performance.…”

Section: Discussionmentioning

confidence: 99%

Toward the Burgeoning Optical Sensors with Ultra‐Precision Hierarchical Structures Inspired by Butterflies

Han

Liu

Chi

et al. 2021

Adv Materials Inter

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In recent years, there has been an increasing interest in the novel bioinspired sensors, especially for their selectivity, response time, and sensitivity. Particular attention is paid to the novel optical functional materials that are crucial for the sensors to achieve various energy transduction. Ideally, many critical parameters of the optical sensing materials can be appropriately tailored to meet various specific requirements using optimal design. Over a long and cruel evolution, nature's creatures have created a variety of photonic structures. One impressive example is the iridescent wings of butterfly. The natural photonic crystals on its surfaces have inspired diverse novel designs of optical functional sensing materials, especially in the past three years. Herein, this review mainly discusses recent advances of the burgeoning butterfly‐inspired sensing materials with optical properties that can be modulated in response to different external stimuli. First, their optical sensing performance to infrared radiation/thermal, vapor, liquid, pH, and so on are discussed in details. Then, the fabrication methods and their working mechanisms are also introduced. Furthermore, the general strategies of these emerging sensing materials and their potential applications are also discussed in‐depth as well as their limitations and the future challenges.

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Structural basis for inhibition of an archaeal CRISPR–Cas type I-D large subunit by an anti-CRISPR protein

Cited by 22 publications

References 55 publications

Insights into the inhibition of type I-F CRISPR-Cas system by a multifunctional anti-CRISPR protein AcrIF24

Insights into the inhibition of type I-F CRISPR-Cas system by a multifunctional anti-CRISPR protein AcrIF24

Structural rearrangements allow nucleic acid discrimination by type I-D Cascade

Toward the Burgeoning Optical Sensors with Ultra‐Precision Hierarchical Structures Inspired by Butterflies

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