2022
DOI: 10.1038/s41594-022-00749-z
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Structural basis of an endocytic checkpoint that primes the AP2 clathrin adaptor for cargo internalization

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Cited by 20 publications
(21 citation statements)
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“…Alternatively, the μ2-adaptin subunit of AP2 has been shown to interact directly with several GPCRs through different motifs 19, 46, 71 , including the canonical tyrosine cargo internalization motif (YXXØ, Ø hydrophobic residue) 72, 73 present at the extreme CTD of PAR1 46 and an unusual stretch of eight arginine residues (rather than the YXXØ) within the CTD of the α 1b -adrenergic receptor 71 . However, in the case of GLP-1R, we reveal a novel motif, TSSLSS, within the middle CTD.…”
Section: Discussionmentioning
confidence: 99%
“…Alternatively, the μ2-adaptin subunit of AP2 has been shown to interact directly with several GPCRs through different motifs 19, 46, 71 , including the canonical tyrosine cargo internalization motif (YXXØ, Ø hydrophobic residue) 72, 73 present at the extreme CTD of PAR1 46 and an unusual stretch of eight arginine residues (rather than the YXXØ) within the CTD of the α 1b -adrenergic receptor 71 . However, in the case of GLP-1R, we reveal a novel motif, TSSLSS, within the middle CTD.…”
Section: Discussionmentioning
confidence: 99%
“…During the dynamic growth phase of clathrin coated pits, clathrin molecules are rapidly deposited at the edge of the growing lattice (Robinson, 2015; Sochacki and Taraska, 2019). Eps15, FCHO, Intersectin and ScEde1 partition to the lattice edge, and are continuously displaced from entering the inner lattice (Mund et al, 2018; Partlow et al, 2022; Sochacki et al, 2017). A similar model has been proposed with TPC confined to the lattice edge in plants (Johnson et al, 2021).…”
Section: Discussionmentioning
confidence: 99%
“…Interactomics and live cell imaging suggest TPC assembles as an octameric complex on the membrane during the initiation of endocytosis (Gadeyne et al, 2014; Wang et al, 2020), with the lipid binding proteins AtEH1, AtEH2, and the muniscin-like protein TML forming an interface with the plasma membrane (Yperman et al, 2021b). In comparison, human Eps15, intersectin and the muniscin FCHo mediate multivalent interactions between themselves, anionic phospholipids (Alaoui et al, 2022; Day et al, 2021; Henne et al, 2010), and AP-2 (Hollopeter et al, 2014; Ma et al, 2015; Partlow et al, 2022) to promote the initiation and growth of clathrin coated pits during endocytosis (Bhave et al, 2020; Cocucci et al, 2012; Lehmann et al, 2019). Thus, AtEH1, AtEH2 and TML may be plant endocytic initiation components.…”
Section: Introductionmentioning
confidence: 99%
“…A recent study examined AP-2 activation by its interaction with the muniscins. Distinct from the straightforward activation caused by the isolated interaction between AP-2 and FCHO1/2, the muniscins cooperate with the membrane to drive AP-2 into a new conformation that likely precedes cargo engagement ( Partlow et al, 2022 ). The interaction with FCHO1/2, which possesses the F-BAR domain that interacts with and bends the membrane, and the membrane interaction of AP-2, emerges as indispensable to trigger the initial invagination of nascent endocytic pit ( Partlow et al, 2022 ).…”
Section: Muniscinsmentioning
confidence: 99%