2024
DOI: 10.1038/s41467-024-54618-y
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Structural basis of deoxynucleotide addition by HIV-1 RT during reverse transcription

Sandra Vergara,
Xiaohong Zhou,
Ulises Santiago
et al.

Abstract: Reverse transcription of the retroviral RNA genome into DNA is an integral step during HIV-1 replication. Despite a wealth of structural information on reverse transcriptase (RT), we lack insight into the intermediate states of DNA synthesis. Using catalytically active substrates, and a blot/diffusion cryo-electron microscopy approach, we capture 11 structures encompassing reactant, intermediate and product states of dATP addition by RT at 2.2 to 3.0 Å resolution. In the reactant state, dATP binding to RT-temp… Show more

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