Structural Basis of the Cross-Striations in Muscle

Hanson, Jean; Huxley, H. E.

doi:10.1038/172530b0

Cited by 352 publications

(193 citation statements)

References 5 publications

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“…Hanson and Huxley (9) suggested that the thick filaments were myosin and that the thin filaments were actin. The localization of myosin in the anisotropic bands has been indicated by other studies (10)(11)(12). In our micrographs of cardiac muscle the thin and thick filaments are evident (Fig.…”

Section: Biopsy Proceduressupporting

confidence: 56%

Experimental Needle Biopsy of the Myocardium of Dogs With Particular Reference to Histologic Study by Electron Microscopy

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Weiss

Hata

et al. 1955

The Journal of Experimental Medicine

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Our experience with needle biopsy of the heart in dogs indicates that myocardial tissue can be sampled one or more times in each animal with comparative safety. Tamponade, pericarditis, serious arrhythmias, or myocardial infarction due to the interruption of coronary vessels was not observed. Excellent specimens were obtained for critical study by light and electron microscopy. Casten and Marsh (1) have used biochemical techniques to study myocardial tissue obtained in similar fashion. Histochemical methods would also be applicable. Although limited to animal studies at present, the technique may conceivably be adapted to the study of human disease. Myocardial puncture has been carried out (20–22) in patients for the recording of intracardiac pressures and for other diagnostic purposes without apparent harm. Our study of the myocardium of dogs by electron microscopy generally confirms the observations of other workers, except that presence of significant numbers of red blood cells in the extravascular spaces of the heart had not been previously described (and is possibly an artifact). Nevertheless, it is notable that the tissue cells, cellular membranes, and intracellular structures appeared to be intact and undistorted in the tissue specimens which were obtained, fixed, and examined by these methods.

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Section: Biopsy Proceduressupporting

confidence: 56%

Experimental Needle Biopsy of the Myocardium of Dogs With Particular Reference to Histologic Study by Electron Microscopy

Price

Weiss

Hata

et al. 1955

The Journal of Experimental Medicine

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“…However, discoid degeneration takes place only in fibrils where the I bands are present. Since this band is very likely composed principally of actin (27), the myosin in the A bands seems to be more resistant to the damage caused by ischemia, as it is more resistant to tryptic digestion (19) and prolonged osmium tetroxide fixation.…”

Section: Observationsmentioning

confidence: 99%

“…5). For more details of normal muscle structure the reader is referred to the literature (19)(20)(21)(22)(23)(24)(25)(26)(27)32). …”

Section: Observationsmentioning

confidence: 99%

Electron Microscopic and Histochemical Observations of Muscle Degeneration After Tourniquet

Moore

Rüska

Copenhaver

1956

The Journal of Cell Biology

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Muscle degeneration and a shock syndrome, which frequently results in death, follow the release of limb tourniquets. Albumin and other plasma components are transferred into the affected muscle tissue (1-4) while toxic products of autolysis and hemolytic substances appear in the circulating blood (5-7). It is possible that this abnormal transfer results in part from damage of capillaries and muscle fiber membranes. The application of cold delays autolysis and fluid exchange, modifies the shock symptoms, and reduces mortality (8). Chemical changes, such as the large decrease in phosphorus (9) and morphologic alterations, as revealed with the light microscope (9, 10), have been carefully studied. Wide interfibrillar spaces are observed in tissues taken immediately after release of the tourniquet, and some fibers show discoid decay of their fibrils. The primary alterations are followed several hours later by advancing granular and hyaline degeneration.The present communication extends the light microscopic observations of the succinic dehydrogenase distribution in normal fibers (11) to the degenerated fibers. With the electron microscope an attempt is made to visualize the finer details in the development of discoid, hyaline, and granular degeneration seen after release of the tourniquet. The alterations thus produced are common to an extremely wide variety of muscle lesions. Further experience will determine whether degenerations of different etiology, such as those caused by bacterial toxins, vitamin E deficiency, or Coxsackie virus infection will expose specific details characteristic of each lesion. MethodsAs in antecedent electrophoretic studies of blood and tissue extracts after shock-producing injuries (2-4), the technique of Rosenthal (12) was used for traumatizafion of hind legs of C57

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“…The structure of cross-striated muscle has been extensively studied in both invertebrate and vertebrate animals, and the contraction of crossstriated muscle has been correlated with the striations and the arrangement of protein filaments within the fibril (7,8,12). The sliding filament mechanism of contraction postulated for crossstriated muscles may hold for some smooth muscles a well (10).…”

Section: Introductionmentioning

confidence: 99%

OBSERVATIONS ON THE FINE STRUCTURE OF PHARYNGEAL MUSCLE IN THE PLANARIAN DUGESIA TIGRINA

MacRae

1963

The Journal of Cell Biology

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Pharyngeal muscle of the planarian Dugesia tigrina was studied by electron microscopy after osmium tetroxide fixation. The muscle cell was observed to contain one myofibril or bundle of myofilaments parallel to its longitudinal axis. The myofilaments were of two types, different in size and distribution. No Z lines or myofilament organization into cross or helical striations were seen. Dense bodies were seen as projections from an invagination of the plasma membrane and as dense lines parallel to the myofilaments. The muscle cells are surrounded by a plasma membrane which is structurally associated with dense body projections, with vesicles and cisternae of sarcoplasmic reticulum, and with synaptic nerve endings. The cell has sarcoplasmic projections perpendicular to its long axis; these projections are seen to contain the nucleus or mitochondria and granules. Mitochondria and granules are also seen in a sarcoplasm rim around the fibril. The dense bodies may serve as attachment for thin myofilaments and function in transmission of stimuli from plasma membrane to the interior of the fibril.

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Structural Basis of the Cross-Striations in Muscle

Cited by 352 publications

References 5 publications

Experimental Needle Biopsy of the Myocardium of Dogs With Particular Reference to Histologic Study by Electron Microscopy

Experimental Needle Biopsy of the Myocardium of Dogs With Particular Reference to Histologic Study by Electron Microscopy

Electron Microscopic and Histochemical Observations of Muscle Degeneration After Tourniquet

OBSERVATIONS ON THE FINE STRUCTURE OF PHARYNGEAL MUSCLE IN THE PLANARIAN DUGESIA TIGRINA

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