Structural basis of the specific interactions of <scp>GRAS</scp> family proteins

Hakoshima, Toshio

doi:10.1002/1873-3468.12987

Cited by 63 publications

(69 citation statements)

References 37 publications

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“…GRAS proteins contain a less conserved, variable Nterminal region, a conserved C-terminal region, and five characteristic domains located in the C-terminal region: LHRI, VHIID, LHRII, PFYRE, and SAW (Hakoshima 2018;Pysh et al 1999). All these conserved domains were found in LaSCL6-var1 and LaSCL6-var2; the shortened 196 aa were in the N-terminal region and did not affect these five GRAS domains (Fig.…”

Section: Identification and Characterization Of Splice Variants Of Lamentioning

confidence: 96%

Regulation of LaSCL6 expression by genomic structure, alternative splicing, and microRNA in Larix kaempferi

Zang

Liu

2019

Tree Genetics & Genomes

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SCR-LIKE-6 (SCL6), a member of the GRAS transcription factor family, plays important roles in many aspects of plant growth and development. In a previous study, we showed that the post-transcriptional regulation of Larix (La)SCL6 by the microRNA miR171 functions in regulating the mode of cell division and the maintenance of embryogenic potential during somatic embryogenesis in larch. To investigate its expression pattern during tree aging, which has been studied by comparative transcriptomic analysis, we reexamined the annotation and expression of every transcript and found one that was longer; we annotated it as SCL6, indicating that it might be a variant of LaSCL6. To verify this, we cloned the DNA and cDNA sequences of this transcript and found that alternative splicing indeed occurred in its expression. Moreover, both variants were detectable in embryogenic cultures and several organs. Notably, the DNA sequence of LaSCL6 contained simple sequence repeats and a single-nucleotide polymorphism which might result in the expression of two variants with a change in their tertiary structures. Regulation of LaSCL6 by miR171 was also found. Taken together, the expression of LaSCL6 is controlled at several levels, and this study not only provides further information about the expression of LaSCL6 but also offers a means to study the regulation of gene expression.

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Section: Identification and Characterization Of Splice Variants Of Lamentioning

confidence: 96%

Regulation of LaSCL6 expression by genomic structure, alternative splicing, and microRNA in Larix kaempferi

Zang

Liu

2019

Tree Genetics & Genomes

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“…Their distribution occurs throughout LHRI, but reside only in the tail end of PFYRE domain. Significantly, the tertiary structure of DELLA proteins consists of an a-helical cap sitting atop of the a/b core, with the entire LHRI domain and the tail end of the PFYRE domain each constituting one-half of the a-helical cap tertiary structure (Hakoshima, 2018). Of the alleles we tested, those found not to increase paclobutrazol resistant (i.e.…”

Section: Making Cereals Resistant To Gibberellin Inhibitors As An Altmentioning

confidence: 99%

“…sln1d.9 and sln1o) occur within the a-helical cap portion of the PFYRE domain (Figure 11) (Chandler and Harding, 2013). The a-helical cap is thought to mediate SLN1 head-to-head homo-and heterodimerization, and known to facilitate interacts with other proteins (Van De Velde et al, 2017b;Hakoshima, 2018). Dozens of direct DELLA binding proteins have been reported in various plant species, from transcriptional activators to chromatin remodelers, and even co-chaperones, with the outcome of DELLA interaction triggering growth suppression (Van De Velde et al, 2017b).…”

Section: Making Cereals Resistant To Gibberellin Inhibitors As An Altmentioning

confidence: 99%

Manipulating Gibberellin Control Over Growth and Fertility as a Possible Target for Managing Wild Radish Weed Populations in Cropping Systems

et al. 2020

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“…Four bZIP transcription factors were present in each of the up-and down-regulated DEG categories, which is interesting because bZIP transcription factors are known to homoand/or heterodimerize with other bZIP proteins. GRAS proteins are important in hormone (gibberellin and jasmonate) signaling, phytochrome signaling and meristem development [64,65], and were present in both the up-(2) and down-regulated (4) DEGs. NAC domain containing proteins, which are involved in various stress signaling pathways and possibly play a role in the interactions between hormones [66,67], were more highly represented in the down-regulated DEGs (15 down DEGs).…”

Section: Genes Differentially Expressed In Transgenic Lines Compared mentioning

confidence: 99%

Transcriptome analysis of responses in Brachypodium distachyon overexpressing the BdbZIP26 transcription factor

2020

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Background: Biotic and abiotic stresses are the major cause of reduced growth, persistence, and yield in agriculture. Over the past decade, RNA-Sequencing and the use of transgenics with altered expression of stress related genes have been utilized to gain a better understanding of the molecular mechanisms leading to salt tolerance in a variety of species. Identification of transcription factors that, when overexpressed in plants, improve multiple stress tolerance may be valuable for crop improvement, but sometimes overexpression leads to deleterious effects during normal plant growth. Results: Brachypodium constitutively expressing the BdbZIP26:GFP gene showed reduced stature compared to wild type plants (WT). RNA-Seq analysis comparing WT and bZIP26 transgenic plants revealed 7772 differentially expressed genes (DEGs). Of these DEGs, 987 of the DEGs were differentially expressed in all three transgenic lines. Many of these DEGs are similar to those often observed in response to abiotic and biotic stress, including signaling proteins such as kinases/phosphatases, calcium/calmodulin related proteins, oxidases/reductases, hormone production and signaling, transcription factors, as well as disease responsive proteins. Interestingly, there were many DEGs associated with protein turnover including ubiquitin-related proteins, F-Box and U-box related proteins, membrane proteins, and ribosomal synthesis proteins. Transgenic and control plants were exposed to salinity stress. Many of the DEGs between the WT and transgenic lines under control conditions were also found to be differentially expressed in WT in response to salinity stress. This suggests that the over-expression of the transcription factor is placing the plant in a state of stress, which may contribute to the plants diminished stature. Conclusion: The constitutive expression of BdbZIP26:GFP had an overall negative effect on plant growth and resulted in stunted plants compared to WT plants under control conditions, and a similar response to WT plants under salt stress conditions. The results of gene expression analysis suggest that the transgenic plants are in a constant state of stress, and that they are trying to allocate resources to survive.

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Structural basis of the specific interactions of GRAS family proteins

Cited by 63 publications

References 37 publications

Regulation of LaSCL6 expression by genomic structure, alternative splicing, and microRNA in Larix kaempferi

Regulation of LaSCL6 expression by genomic structure, alternative splicing, and microRNA in Larix kaempferi

Manipulating Gibberellin Control Over Growth and Fertility as a Possible Target for Managing Wild Radish Weed Populations in Cropping Systems

Transcriptome analysis of responses in Brachypodium distachyon overexpressing the BdbZIP26 transcription factor

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