Using bolts as shear connectors of the steel-concrete composite beams enables prefabricated assembly and makes it possible to demountable and reuse the prefabricated components. However, studies on the fire resistance of prefabricated demountable composite beams (PDCBs) connected by bolts are limited. In this paper, four full-scale fire resistance tests were conducted to investigate the fire resistance of PDCBs connected by shear bolts with profiled sheet ribs parallel to the steel beam. Test phenomena, heating curves, deformation curves, and critical temperatures of specimens were obtained through fire-resistant tests. Test results show that the shear failure of bolted connectors occurred in the partially connected PDCBs instead of in the fully connected ones. Under the same load ratio, the fire resistance limit of PDCBs with fire protection was 4.4 times that of PDCBs without fire protection. The temperatures of the upper flanges of the steel beams in the PDCBs were overestimated by the existing codes. Moreover, numerical heat transfer analysis was carried out to investigate the effect of steel beam upper flange dimensions and concrete slab thickness on the temperature distribution of the steel beam upper flange. Finally, formulas were proposed for calculating the temperature distribution of the steel beam upper flange, which agrees well with the FEM simulated values.