Structural changes and immunohistochemical localisation of epidermal growth factor receptor in the true vocal fold of female albino rats administered anabolic, androgenic steroids, and effects of anti-androgen therapy

Amer, H E; Asker, Samar A.; Mazroa, Shireen A.

doi:10.1017/s0022215111000831

Cited by 8 publications

(11 citation statements)

References 33 publications

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“…Administration of anabolicandrogenic steroids to female rats results in thickening of the vocal folds, including the lamina propria (48). This was in agreement with our experiment results, in which castration caused a decrease in vocal cord ECM expression in the vocal fold lamina propria.…”

Section: Discussionsupporting

confidence: 92%

Differential Responses to Steroid Hormones in Fibroblasts From the Vocal Fold, Trachea, and Esophagus

et al. 2015

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There is accumulating evidence that fibroblasts are target cells for steroids such as sex hormones and corticoids. The characteristics of fibroblasts vary among tissues and organs. Our aim in this study is to examine differences in responses to steroid hormones among fibroblasts from different cervicothoracic regions. We compared the actions of steroid hormones on cultured fibroblasts from the vocal folds, which are considered to be the primary target of steroid hormones, and the trachea and esophagus in adult male rats. Expression of steroid hormone receptors (androgen receptor, estrogen receptor α, and glucocorticoid receptor) was identified by immunofluorescence histochemistry. Androgen receptor was much more frequently expressed in fibroblasts from the vocal fold than in those from the trachea and esophagus. Cell proliferation analysis showed that administration of testosterone, estradiol, or corticosterone suppressed growth of all 3 types of fibroblasts. However, mRNA expression for extracellular matrix-associated genes, including procollagen I and III and elastin, and hyaluronic acid synthase I was elevated only by addition of testosterone to fibroblasts from the vocal fold. These results indicate that each steroid hormone exerts region-specific effects on cervicothoracic fibroblasts with different properties through binding to specific receptors.

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Section: Discussionsupporting

confidence: 92%

Differential Responses to Steroid Hormones in Fibroblasts From the Vocal Fold, Trachea, and Esophagus

et al. 2015

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“…Although the TA muscles may not be estrogen dependent, other sex hormones likely influence the neuromuscular parameters of the vocal folds. For example, TA muscle fibers hypertrophy with administration of androgens, consistent with our previous finding that male rats have larger myofiber sizes than females, regardless of the individual rat's weight 25,39 . This may also explain why men have a greater rate of atrophy of the TA muscles in old age compared to women 2,40,41 .…”

Section: Discussionsupporting

confidence: 88%

“…For example, TA muscle fibers hypertrophy with administration of androgens, consistent with our previous finding that male rats have larger myofiber sizes than females, regardless of the individual rat's weight. 25,39 This may also explain why men have a greater rate of atrophy of the TA muscles in old age compared to women. 2,40,41 Although the outer connective layers of the vocal folds may be influenced by estrogen levels, the TA muscles may be more influenced by androgen levels.…”

Section: Discussionmentioning

confidence: 99%

The Effects of Menopause on Neuromuscular Parameters of the Rat Vocal Folds

Lenell

Johnson

2020

The Laryngoscope

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Objectives/Hypothesis: Menopause adversely affecs power and endurance of the limb muscles. However, despite clinical observations that menopause corresponds to negative changes of the voice, the direct effects of estrogen deprivation on the thyroarytenoid muscles are unknown. The purpose of this study was to investigate the effects of estrogen deprivation via ovariectomy on three neuromuscular parameters of the thyroarytenoid muscles using a rat model.Study Design: Animal model. Methods: Cryosections of vocal folds of 20 (10 control and 10 ovariectomized) female rats were stained to label neuromuscular junctions, fiber size, or parvalbumin levels using immunohistochemical techniques and compared between experimental groups.Results: The neuromuscular junctions, thyroarytenoid fiber sizes, and parvalbumin levels of the vocal folds were similar between experimental groups.Conclusions: The loss of estrogen did not change neuromuscular parameters of the vocal folds of adult female rats; therefore, vocal changes within the outer vibratory layers of the vocal folds may primarily be responsible for clinically observed menopausal vocal changes.

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“…To explain these irreversible changes, Amer and al. demonstrated structural and immunophysiological changes in an animal model after 2 months of systemic anabolic steroid treatment . The rat model vocal folds showed thicker epithelial layers with increased mitotic figures, thicker lamina propria, and thicker muscle fibers.…”

Section: Discussionmentioning

confidence: 95%

Case report: The long‐term effects of anabolic steroids on the female voice over a 20‐year period

Bensoussan

Anderson

2019

Clinical Case Reports

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Structural changes and immunohistochemical localisation of epidermal growth factor receptor in the true vocal fold of female albino rats administered anabolic, androgenic steroids, and effects of anti-androgen therapy

Abstract: Anabolic steroids caused structural and immunohistochemical changes within the female rat true vocal fold. Co-administration of anti-androgens did not prevent these changes, suggesting that anti-androgens have a limited role in the management of such changes in humans.

Cited by 8 publications

References 33 publications

Differential Responses to Steroid Hormones in Fibroblasts From the Vocal Fold, Trachea, and Esophagus

Differential Responses to Steroid Hormones in Fibroblasts From the Vocal Fold, Trachea, and Esophagus

The Effects of Menopause on Neuromuscular Parameters of the Rat Vocal Folds

Case report: The long‐term effects of anabolic steroids on the female voice over a 20‐year period

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