Structural Changes During Development in Bovine Fetal Epiphyseal Cartilage

Pal, Subhash; Tang, Lih-Heng; Choi, Hong‐Keun; Habermann, Edward T.; Rosenberg, Lawrence; Roughley, Peter J.; Poole, A R

doi:10.1016/s0174-173x(81)80017-9

Cited by 94 publications

(72 citation statements)

References 22 publications

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“…DNA was converted to cell number by using a conversion constant of 7.7 pg of DNA per cell (36). Hydroxyproline was converted to collagen by assuming a mass ratio of collagen: hydroxyproline equal to 7.25:1 (17,37). Portions of spent medium were analyzed for GAG (33).…”

Section: Methodsmentioning

confidence: 99%

“…Fetal and postnatal growth of the articular cartilage normally involves a net deposition of collagen that is greater than that of proteoglycan, as well as an increase in mechanical integrity. During maturation of articular cartilage from fetus to skeletal maturity, there is an increase in collagen and pyridinoline (Pyr) crosslink densities, but little or no change in the content of glycosaminoglycans (GAGs) (14,(16)(17)(18). These biochemical changes are accompanied by an increase in tensile modulus and strength (15).…”

mentioning

confidence: 99%

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Mechanisms of cartilage growth: Modulation of balance between proteoglycan and collagen in vitro using chondroitinase ABC

Asanbaeva¹,

Masuda²,

Thonar³

et al. 2007

Arthritis & Rheumatism

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Objective. To examine the cartilage growthassociated effects of a disruption in the balance between the swelling pressure of glycosaminoglycans (GAGs) and the restraining function of the collagen network, by diminishing GAG content prior to culture using enzymatic treatment with chondroitinase ABC.Methods. Immature bovine articular cartilage explants from the superficial and middle layers were analyzed immediately or after incubation in serumsupplemented medium for 13 days. Other explants were treated with chondroitinase ABC to deplete tissue GAG and also either analyzed immediately or after incubation in serum-supplemented medium for 13 days. Treatment-and incubation-associated variations in tissue volume, contents of proteoglycan and collagen network components, and tensile mechanical properties were assessed.

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Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

Mechanisms of cartilage growth: Modulation of balance between proteoglycan and collagen in vitro using chondroitinase ABC

Asanbaeva¹,

Masuda²,

Thonar³

et al. 2007

Arthritis & Rheumatism

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“…The hydroxyproline content was converted to that of total collagen using a mass ratio of 7.25. 33 The optical density was detected with a microplate reader at 560 nm (Bio-Tek). A standard curve was established using a series of concentrations of hydroxyproline.…”

Section: Characterizations Of Acellular Ecm Scaffoldsmentioning

confidence: 99%

Development and Characterization of Acellular Extracellular Matrix Scaffolds from Porcine Menisci for Use in Cartilage Tissue Engineering

Chen

Jhan

et al. 2015

Tissue Engineering Part C: Methods

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“…In experiment 2, HA content was determined for individual samples. Hydroxyproline content was converted to COL content by assuming a mass ratio of collagen to hydroxyproline equal to 7.25 for bovine cartilage [58,59] and 7.1 for human cartilage [60]. The molar ratio of PYR per collagen molecule was calculated, assuming the molecular weight of collagen triple helix of 300,000.…”

Section: Biochemical Analysismentioning

confidence: 99%

Articular cartilage tensile integrity: Modulation by matrix depletion is maturation-dependent

Asanbaeva

Tam

Schumacher

et al. 2008

Archives of Biochemistry and Biophysics

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Articular cartilage function depends on the molecular composition and structure of its extracellular matrix (ECM). The collagen network (CN) provides cartilage with tensile integrity, but must also remodel during growth. Such remodeling may depend on matrix molecules interacting with the CN to modulate the tensile behavior of cartilage. The objective of this study was to determine the effects of increasingly selective matrix depletion on tensile properties of immature and mature articular cartilage, and thereby establish a framework for identifying molecules involved in CN remodeling. Depletion of immature cartilage with guanidine, chondroitinase ABC, chondroitinase AC, and Streptomyces hyaluronidase markedly increased tensile integrity, while the integrity of mature cartilage remained unaltered after depletion with guanidine. The enhanced tensile integrity after matrix depletion suggests that certain ECM components of immature matrix serve to inhibit CN interactions and may act as modulators of physiological alterations of cartilage geometry and tensile properties during growth/maturation.

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Structural Changes During Development in Bovine Fetal Epiphyseal Cartilage

Cited by 94 publications

References 22 publications

Mechanisms of cartilage growth: Modulation of balance between proteoglycan and collagen in vitro using chondroitinase ABC

Mechanisms of cartilage growth: Modulation of balance between proteoglycan and collagen in vitro using chondroitinase ABC

Development and Characterization of Acellular Extracellular Matrix Scaffolds from Porcine Menisci for Use in Cartilage Tissue Engineering

Articular cartilage tensile integrity: Modulation by matrix depletion is maturation-dependent

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