Structural Changes in Spleen Architecture upon Plasmodium berghei (NK-65) Infection in BALB/c Mice

Kumar, Vineet; Bagai, Upma

doi:10.9790/3008-09431620

Cited by 5 publications

(3 citation statements)

References 16 publications

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“…The malaria parasite is considered a foreign body when it enters the host and is immediately recognized by a specific immune response. This causes the proliferation of the splenic white pulp (lymphocytes, macrophages, and reticular cells increase in size) so that the diameter of the splenic white pulp also increases [ 49 ]. Consequently, the spleen in organisms infected with malaria will enlarge as a result of increased erythropoiesis and hematopoiesis [ 50 ].…”

Section: Discussionmentioning

confidence: 99%

Oral Administration of Piperine as Curative and Prophylaxis Reduces Parasitaemia in Plasmodium berghei ANKA-Infected Mice

Khairani

Fauziah

Wiraswati

et al. 2022

Journal of Tropical Medicine

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Malaria remains a public health problem and a leading cause of death worldwide. Consequently, the discovery of novel agents, including substances from medicinal plants, is urgently needed. Piper nigrum has long been used by the community in the treatment of the symptoms of malaria. In a previous study, Piper nigrum was demonstrated to exhibit promising antiplasmodial activity against Plasmodium falciparum 3D7 and INDO strains. The aim of this study was to further investigate the antimalarial activity (curative and prophylactic) of piperine (a major isolated constituent of Piper nigrum) in Plasmodium berghei ANKA-infected mice. Piperine 10, 20, and 40 mg/kg body weight (bw), artesunate 5 mg/kg bw, and DMSO were administered orally for four days to different groups of Swiss Webster mice. Then, mice were monitored for parasitaemia, body weight, rectal temperature, survival rate, and clinical parameters. Piperine 40 mg/kg bw in curative and prophylactic tests had the maximum parasitaemia chemosuppression of 79.21% and 58.8% (p < 0.05), respectively, with a significant effect on the survival rate compared with control animals. In the curative test, piperine 40 mg/kg bw reduced the mean clinical score compared with the control group. Additionally, piperine showed an ability to protect organs (lungs, liver, spleen, and kidneys) from some damage in a dose-dependent manner. This study can be used as a basis for further discovery of novel chemotherapeutic or chemoprophylactic compounds.

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Section: Discussionmentioning

confidence: 99%

Oral Administration of Piperine as Curative and Prophylaxis Reduces Parasitaemia in Plasmodium berghei ANKA-Infected Mice

Khairani

Fauziah

Wiraswati

et al. 2022

Journal of Tropical Medicine

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“…The blood cells like macrophages are found in sinuses, as are plasma cells. The splenic cords contained lymphocytes and monocytes (T-cells) in high level [7].…”

Section: Discussionmentioning

confidence: 99%

Splenohepatomegaly in Protein-Malourished Mice Infected with Plasmodium Vinckei

2020

AIDT

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Cellular and histopathological observations were made with light microscopy during the Plasmodium vinckeiinfection in protein-malnourished mice (fed on 2% casein diet and para-aminobenzoic acid deficient diet (PABA-DD).In the infected spleen, hyperplasia of the red as well as the white pulp was evident; active erythropoiesis and lymphopoiesis. Hyperemia and deposition of malarial pigments inside the macrophages. Cloudy swelling was noticed in the swollen hepatic cells of the uninfected liver due to protein deficiency. This lesion had developed to fatty infiltration in the infected protein-malnourished mice. These new and interesting changes represent the combined deleterious effect upon the host of infection and protein-malnutrition. Hypertrophy of the kupffer cells due to progressive phagocytosis of malarial pigments. In addition, focal necrosis surrounded by inflammatory cells was seen.

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“…Mouse spleens infected with malaria undergo expansion of white pulp, loss of structure in the germinal center, activation of T and B cells, and loss of marginal zones in the white pulp so that the boundaries of white and red pulp become blurred (Carvalho et al, 2007;Kumar and Bagai, 2014). B cells and antibodies play an important role in the development of immunity against malaria infection.…”

Section: Advances In Animal and Veterinary Sciencesmentioning

confidence: 99%

The Oxidative Stress-Mediated Effects in Pregnant Mice with Plasmodium berghei Infection

Syukriah¹,

Faizah²,

Saragih³

et al. 2021

AAVS

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Malaria during pregnancy can lead to various pathological conditions for pregnant women and fetuses such as fever, abortion, low birth weight, and fetal death. In this study, we examined differences in oxidative stress-mediated effects in pregnant and offspring mice in Plasmodium berghei infection. A Complete randomized design was used in this study, in which 25 mice were divided into five groups. Group 1 as a control group, consisted of non-pregnant and Plasmodium-uninfected mice, group 2 comprised pregnant and uninfected mice, group 3 to 5 were pregnant mice and were infected with 1 x 10 1 iRBCs, 1 x 10 2 iRBCs, and 1 x 10 3 iRBCs respectively. On the fourth day of post-treatment, the parasite level was calculated. Malondialdehyde (MDA) levels of mother's and offspring's liver and spleen were observed by using the TBARS Assay Kit, also the superoxide dismutase activities. As supporting data the histological analysis of the mother's and offspring's liver and spleen were prepared by using the paraffin method. There were sigificant results in the parasites levels and the increase in P. berghei infection followed an increase of oxidative stress in mothers and offspring mouse in the treatment and control groups. The liver and spleen of mothers have been affected with P. berghei infection, however, there are still no effects on the offsprings, and the body weight of the offsprings from infected mothers were lower than uninfected mothers. This study revealed that P. berghei infection had different effects in oxidative stress-mediated in pregnant mice, but not their offspring.

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Structural Changes in Spleen Architecture upon Plasmodium berghei (NK-65) Infection in BALB/c Mice

Cited by 5 publications

References 16 publications

Oral Administration of Piperine as Curative and Prophylaxis Reduces Parasitaemia in Plasmodium berghei ANKA-Infected Mice

Oral Administration of Piperine as Curative and Prophylaxis Reduces Parasitaemia in Plasmodium berghei ANKA-Infected Mice

Splenohepatomegaly in Protein-Malourished Mice Infected with Plasmodium Vinckei

The Oxidative Stress-Mediated Effects in Pregnant Mice with Plasmodium berghei Infection

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