Structural Changes in the Brain of Rats With Experimental Allergic Encephalomyelitis After Cryopreserved Mesenchymal Stem Cells Impact

Semenova, V. M.; Tsymbalyuk, V. І.; Любич, Л. Д.; Egorova, D. M.; Stayno, L. P.; Shevchuk, O.V.; Vaslovich, V. V.; Verbovska, S. A.; Deryabina, О. G.; Shuvalova, N. S.; Pichkur, L. D.

doi:10.26724/2079-8334-2020-4-74-199-204

Cited by 2 publications

(2 citation statements)

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“…Transplantation of hMMSCs to the cuprizone-treated mice decreased the MDA content in brain and caused certain positive morpho-functional changes in CNS. Our morphological data are consistent with the results of other authors [22]. The antioxidant properties of hMMSCs in the demyelinating pathology were shown in [1,23].…”

Section: Influence Of Hmmscs Transplantation On the Behavior And Oxidative Stress In The Brain Of Cuprizone-treated Micesupporting

confidence: 93%

Melatonin and fibroblast growth factor-2 potentiate the effects of human umbilical cord multipotent mesenchymal stromal cells in mice with cuprizone-induced demyelination

et al. 2021

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To study the influence of melatonin and recombinant human fibroblast growth factor (rhFGF-2) on human umbilical cord multipotent mesenchymal stromal cells (hMMSCs) effects at experimental demyelination. Methods. Adult mice were fed with neurotoxin cuprizone for 3 weeks. hMMSCs (5×10 5 cells) were injected on the 10th day of cuprizone diet. Injections of melatonin or rhFGF-2 were started on the 11th day of cuprizone diet. We used cell culture, flow cytometric, spectrophotometric and histological methods, "open field" and "rotarod" tests. Results. Under the cuprizone influence the motor-, emotional activities and muscle tone decreased. The malondialdehyde (MDA) content in brain increased while the activity of antioxidant enzymes decreased. After injection of hMMSCs the number of crossed squares and grooming activity increased while MDA content decreased. Melatonin and rhFGF-2 injections enhanced the effect of cells on grooming activity and increased the glutathione reductase activity. Melatonin also increased the number of boluses, muscle tone and glutathione peroxidase activity. Conclusion. Melatonin and rhFGF-2 improve the effect of hMMSCs in cuprizonetreated mice. The effects of hMMSCs and melatonin combination is greater than that with rhFGF-2.

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Section: Influence Of Hmmscs Transplantation On the Behavior And Oxidative Stress In The Brain Of Cuprizone-treated Micesupporting

confidence: 93%

Melatonin and fibroblast growth factor-2 potentiate the effects of human umbilical cord multipotent mesenchymal stromal cells in mice with cuprizone-induced demyelination

et al. 2021

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“…Багатогранний позитивний ефект МСК пов'язують із патотропним хомінгом [97], явищем нейрогенного транс диференціювання [90,[97][98][99], зокрема в нейролемоцити [100][101][102], здатністю до злиття з клітинами реципієнта [103], мікровезикулярним [104][105][106][107][108], факторним [97,[100][101][102] чи контактним [97,105] впливом. Крім того, трансплантовані МСК мають імуносупресивну і, ймовірно, локальну протизапальну дію [109][110][111][112].…”

Section: мезенхімальні стовбурові клітини при лікуванні травми перифе...unclassified

Peripheral nerve injury: molecular pathophysiology and prospects for restorative treatment by means of cell transplantation: a literature review

Melikov,

Medvediev

2023

Ukr Neurosurg J

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Peripheral nerve injury (PNI) is a fairly common pathology—PNI accounts for 1-5% of all peacetime injuries and 12% of all combat injuries. This injury leads to disability, the development of chronic pain syndromes and a significant deterioration in the quality of life of the victims. Unfortunately, at present, in the case of the most frequent type of combat trauma — damage to the limbs — PNI treatment is mostly done last, "on the residual principle." Modern means of surgical and conservative treatment of PNI do not provide complete restoration of lost functions, therefore, restorative treatment of PNI is an urgent biomedical problem. The article reviews the currently known molecular mechanisms of various stages of PNI, as well as the plasticity of the central parts of the nervous system on the background of this injury. The main reasons for the limitation of autogenous recovery of functions after a sustained PNI are described — the absence of a relevant spatial organization of regrowth of axons in the area of PNI; post-traumatic death of neurons of spinal nodes and central parts of the nervous system; failure of plastic reconstruction of brain and spinal cord neural networks; irreversibility of atrophy of denervated muscles. Based on this, it was established that the means of restorative treatment of PNI should touch not only the epicenter of PNI, but also the central parts of the nervous system and denervated muscles. Mesenchymal stem cells (MSCs) are well-known means of a positive influence on the the restorative process in the focus of PNI, as well as a source of supportive influence/ strengthening effect and an amplifier of the plasticity of brain neural networks, which makes these cells a promising element of bioengineering treatment of PNI. The effect of MSCs on the central parts of the nervous system in case of PNI remains the least studied. Data from the literature indicate that such an effect can provide support for secondarily affected neurons and stimulate the plastic reorganization of brain networks, i.e., in general, significantly improve the results of restorative treatment of PNI.

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Structural Changes in the Brain of Rats With Experimental Allergic Encephalomyelitis After Cryopreserved Mesenchymal Stem Cells Impact

Cited by 2 publications

References 8 publications

Melatonin and fibroblast growth factor-2 potentiate the effects of human umbilical cord multipotent mesenchymal stromal cells in mice with cuprizone-induced demyelination

Melatonin and fibroblast growth factor-2 potentiate the effects of human umbilical cord multipotent mesenchymal stromal cells in mice with cuprizone-induced demyelination

Peripheral nerve injury: molecular pathophysiology and prospects for restorative treatment by means of cell transplantation: a literature review

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