Structural characterisation of a complex heteroglycan from the cyanobacterium Nostoc commune

Jensen, S.; Petersen, Bent O.; Ómarsdóttir, Sesselja; Paulsen, Berit Smestad; Duus, Jens Øllgaard; Ólafsdóttir, Elín Soffia

doi:10.1016/j.carbpol.2012.08.063

Cited by 35 publications

(19 citation statements)

References 28 publications

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“…The average molecular weight was estimated to be 1.5×10 6 g/mol. Structural characterization of the heteroglycan was performed by high‐field NMR spectroscopy as well as monosaccharide analysis after methanolysis by GC and supported by linkage analysis by GC‐MS …”

Section: Extraction and Properties Of Cyanobacterial Polysaccharidesmentioning

confidence: 76%

“…Most of EPSs are anionic because of the presence of uronic acids and/or sulfated sugars. On the other hand, Nostoc commune , which has much higher amount of EPS than other various gelatinous organisms such as seaweed, produces EPS comprising cationic groups of amine‐containing sugars. Due to their multi‐functionality, the EPSs adsorb heavy metal ions in the water to prevent toxicity, and protect the cells themselves.…”

Section: Overview Of Cyanobacterial Polysaccharidesmentioning

confidence: 90%

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Development of Functional Bionanocomposites Using Cyanobacterial Polysaccharides

Okajima

Sornkamnerd

Kaneko

2018

The Chemical Record

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Cyanobacteria are regarded as very eco-friendly microreactors for the production of various biomolecules such as polysaccharides by fixing not only carbon but also nitrogen in water. Cyanobacterial polysaccharides having various functional groups such as hydroxyls, carboxyls, sulfates, etc. have the ability to interact with metals or inorganics, to create bionanocomposites. Sacran, a supergiant cyanobacterial anionic polysaccharide extracted from the extracellular matrix of Aphanothece sacrum which is mass-cultivated in freshwater, is mainly used to create functional bionanocomposites. Gel-type bionanocomposites of sacran with various metal cations are formed and showed photoresponsive functions. Metal recovery is performed from the sacran bionanocomposite gels. Sacran chains are complexed with multi-wall carbon nanotubes (MWCNT) to give viscose dispersion from which MWCNT bionanocomposites can be collected by electrophoresis. The MWCNT/sacran dispersion retains the capability of adsorbing various metal ions to form hardened hydrogel beads. Finally, natural inorganic sepiolite can be used for sacran bionanocomposites which show an efficient neodymium ion adsorption ability. Thus, cyanobacterial polysaccharides are useful for preparing eco-friendly and functional bionanocomposites with various hard materials.

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Section: Extraction and Properties Of Cyanobacterial Polysaccharidesmentioning

confidence: 76%

Section: Overview Of Cyanobacterial Polysaccharidesmentioning

confidence: 90%

Development of Functional Bionanocomposites Using Cyanobacterial Polysaccharides

Okajima

Sornkamnerd

Kaneko

2018

The Chemical Record

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“…Cyanobacteria are widespread photo-autotrophic microorganisms that combine the ability to perform aerobic photosynthesis with prokaryotic characteristics. Some species also have the ability to fix atmospheric N (Pereira et al 2009;Jensen et al 2013). Cyanobacteria of the genus Nostoc are found in many terrestrial and aquatic ecosystems, and their ability to fix N enhances the quality of nutrient-poor soils (Potts 2000).…”

Section: Discussionmentioning

confidence: 99%

Expression of thenifHgene in diazotrophic bacteria inEucalyptus urograndisplantations

et al. 2016

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A large proportion of eucalypt plantations in Brazil are located in areas with low soil fertility. The actions of microorganisms are of great importance for the cycling of nutrients, including nitrogen (N), that are essential for plant metabolism. Denaturing gradient gel electrophoresis (DGGE) was used to monitor and identify the total and active microorganisms involved in the N cycle in both the soil and root systems of a forest of Eucalyptus urograndis with sections that were fertilized with N or unfertilized. Quantitative real-time PCR was used to examine the expression of the nifH gene in N-fixing bacteria present in both the soil and root systems. According to the DGGE analysis, in the total and active populations of N-fixing bacteria, the presence and expression of the nifH gene were influenced by the winter and summer seasons and (or) N fertilization, respectively. DGGE band sequencing from total DNA samples showed that the most abundant group of diazotrophic bacteria belonged to Alphaproteobacteria in both the soil and root systems. Quantitative real-time PCR revealed that nifH expression was higher in the soil samples, especially in those that did not receive N fertilization. The differences in the composition of the total and active diazotrophic populations highlight the importance of evaluating the active populations, because they are effectively responsible for the biogeochemical transformation of N and also control its’ availability to plants.

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“…This could be due to the complexity of cyanobacterial EPS. As a characteristic feature, up to 12 different monosaccharides have been identified in cyanobacterial EPS (Pereira et al, 2009), and the partially characterized structures of N. commune EPS are highly complex (Brüll et al, 2000;Helm et al, 2000;Huang et al, 1998;Jensen et al, 2013;Kanekiyo et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Characterization of extracellular matrix components from the desiccation-tolerant cyanobacterium <i>Nostoc commune</i>

Inoue-Sakamoto

Tanji

Yamaba

et al. 2018

J. Gen. Appl. Microbiol.

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The terrestrial cyanobacterium Nostoc commune forms macroscopic colonies in its natural habitats, and these colonies consist of both cellular filaments and massive extracellular matrixes. In this study, the biochemical features of the extracellular matrix components were investigated. Naturally growing N. commune was tolerant to desiccation, and produced massive extracellular polysaccharides that contained both neutral sugars and glucuronic acid as constituent monosaccharides. The extracellular polysaccharide contents and desiccation tolerance were compared in laboratory culture strains of Nostoc species. The laboratory culture of N. commune strain KU002 was sensitive to desiccation and produced smaller amounts of extracellular polysaccharides, unlike the field-isolated naturally growing colonies. Nostoc punctiforme strain M-15, which is genetically closed to N. commune, was able to tolerate desiccation, although the other Nostoc strains were desiccation-sensitive. A laboratory culture strain of the aquatic cyanobacterium Nostoc sphaericum produced massive extracellular polysaccharides but was sensitive to desiccation, suggesting that extracellular matrix production is not enough to make this strain tolerant to desiccation. WspA (water stress protein) and SodF (superoxide dismutase) were found to be characteristic protein components of the extracellular matrix of N. commune. Because the WspA proteins were heterogeneous, the wspA genes were highly diverse among the different genotypes of N. commune, although the sodF gene was rather conservative. The heterogeneity of the WspA proteins suggests their complex roles in the environmental adaptation mechanism in N. commune.

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Structural characterisation of a complex heteroglycan from the cyanobacterium Nostoc commune

Cited by 35 publications

References 28 publications

Development of Functional Bionanocomposites Using Cyanobacterial Polysaccharides

Development of Functional Bionanocomposites Using Cyanobacterial Polysaccharides

Expression of thenifHgene in diazotrophic bacteria inEucalyptus urograndisplantations

Characterization of extracellular matrix components from the desiccation-tolerant cyanobacterium <i>Nostoc commune</i>

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