Structural characterization and anti-tumor activity in vitro of a water-soluble polysaccharide from dark brick tea

Structural Characterization and Macrophage Polarization-Modulating Activity of a Novel Polysaccharide from Large Yellow Tea

“…In this study, we successfully isolated and purified a new homogeneous polysaccharide LYP-S3 from LYP under the guidance of the activity evaluation based on the screening of the M2 polarization of ATMs. The newly acquired LYP-S3 has a narrow molecular weight of 2.86 × 10 4 Da, which is remarkably different from previously reported tea polysaccharides . Moreover, evident differences in monosaccharide compositions and molar ratios were found between LYP-S3 and other tea polysaccharides.…”

“…The newly acquired LYP-S3 has a narrow molecular weight of 2.86 × 10 4 Da, which is remarkably different from previously reported tea polysaccharides. 33 Moreover, evident differences in monosaccharide compositions and molar ratios were found between LYP-S3 and other tea polysaccharides. LYP-S3 is a new type of active acidic polysaccharide with lower molecular weight and higher uronic acid content, the main skeleton of which was proposed to be a novel straight chain with α-1,4-linked GalpA with Oacetyl.…”

A Review on the Extraction, Bioactivity, and Application of Tea Polysaccharides

“…Liu et al extracted a water-soluble homogeneous polysaccharide (DTP-1) from dark brick tea, which could effectively inhibit cancer cell proliferation, induce apoptosis, and suppress migration. However, DTP-1 did not affect normal cells [ 46 ]. Park et al treated peritoneal macrophages of mice with purified polysaccharide GTE-II from mature leaves of green tea and found that GTE-II can increase cytokine production in macrophages and inhibit the growth and metastasis of tumors, indicating that GTE-II has anti-tumor effects [ 47 ].…”

Advances in the Utilization of Tea Polysaccharides: Preparation, Physicochemical Properties, and Health Benefits

“…In recent years, TPS has received extensive attention due to its broad therapeutic properties and relatively low toxicity to normal cells, and it is expected to become an alternative or adjunct to traditional anticancer drugs [ 102 ]. Liu et al, isolated and purified a water-soluble homogeneous polysaccharide (DTP-1) from dark brick tea, and then they evaluated the cytotoxic activity of DTP-1 on cancer cells and normal cells in vitro [ 103 ]. The results showed that DTP-1 had significant in vitro anti-tumor effects, especially on A549 and SMMC7721 cells, and the inhibitory effect of DTP-1 on cancer cell proliferation was positively correlated with its dose.…”

“…Ziyang green tea Ameliorating high-fructose diet-induced pancreatic β-cell damage and inhibiting hepatic steatosis and oxidative damage [112] Ziyang green tea Mediating antioxidant and free radical scavenging, thereby effectively preventing liver damage [113] Green tea Promoting superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activity in blood, liver, and heart [114] Huangshan Maofeng Inhibiting lipid peroxidation while promoting the body's antioxidant activity to protect the liver [23] Longjing 43 tea flower Inhibiting the elevation of serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels, reducing the formation of malondialdehyde (MDA), and simultaneously enhancing the activities of SOD and GPx to reduce liver damage [98] Keemun black tea Improving the enzymatic and non-enzymatic antioxidant defense system to protect the liver, thereby effectively alleviating the production of free radicals in the body and inhibiting lipid peroxidation in liver tissue [24] Antitumor activity Dark brick tea Inhibiting cancer cell proliferation and migration and inducing cancer cell apoptosis [103] Ziyang green tea Inhibiting the proliferation of human osteosarcoma cells (U-2 OS) [104] Green tea Targeting lysosomes and activated caspase-9/-3 via the lysosome-mitochondrial pathway to induce apoptosis in colon cancer cells (CT26) [105] Tea flowers Inhibiting the proliferative activity of human gastric cancer cells (BGC-823) [98] Green tea Increasing the levels of SOD, CAT, and GPx while inhibiting lipid peroxidation and pro-inflammatory cytokine levels from attenuating oxidative damage and inflammatory responses [115] Ziyang green tea Inhibiting the proliferation of osteosarcoma cells in vitro and the growth of tumor volume and tumor weight in vivo [104] Green and black teas Inhibiting pulmonary neutrophil recruitment and oxidative tissue damage, resulting in higher anti-inflammatory effects and resistance to murine sepsis [116] Oolong tea Inhibiting tumor growth, reducing liver toxicity and nephrotoxicity, stimulating the body's antioxidant activity and immune function, and finally achieving an anti-liver cancer effect [75] Green tea Increasing the Bcl2-associated X protein (Bax)/B-cell lymphoma-2 (Bcl-2) ratio, elevating caspase-3 protein expression, and decreasing miR-93 expression in prostate cancer cells [54] Immunostimulatory activity…”