Structural Characterization and Anticancer Activity of Cell-Bound Exopolysaccharide from Lactobacillus helveticus MB2-1

Abstract

A novel cell-bound exopolysaccharide (c-EPS) was isolated from Lactobacillus helveticus MB2-1 by ultrasonic extraction, anion exchange, and gel filtration chromatography before being structurally characterized. The c-EPS is a heteropolysaccharide with an average molecular weight of 1.83 × 10(5) Da and is composed of glucose, mannose, galactose, rhamnose, and arabinose at a molar ratio of 3.12:1.01:1.00:0.18:0.16. Methylation analysis and nuclear magnetic resonance analysis revealed that the c-EPS is a linear glucomannogalactan containing repeating units of → 6)-β-D-Manp-(1 → 3)-β-D-Glcp-(1 → 3)-β-D-Glcp-(1 → 3)-β-D-Glcp-(1 → 4)-α-D-Galp-(1 → and trace amounts of Rhap-(1 → and (1 → 4)-Arap residues. Complex formation with Congo red demonstrated a triple-strand helical conformation for the c-EPS. Scanning electron microscopy of the c-EPS revealed many regular feather-like structural units. Topographical examination of c-EPS by atomic force microscopy revealed that the c-EPS formed rounded-to-spherical lumps with different sizes and chain formations. Furthermore, preliminary in vitro tests revealed that c-EPS significantly inhibited the proliferation of HepG-2, BGC-823, and especially HT-29 cancer cells.

Isolation and characterisation of lactic acid bacteria from traditional koumiss and kurut

“…Similarly, heteropolymeric EPS production was shown in other L. helveticus strain. [21] These results clearly show that EPS production in koumiss and kurut LAB strains is an important characteristic that these strains harbour which can affect the technological properties of these products.…”

Characterization of Exopolysaccharide Produced byStreptococcus thermophilusCC30

“…Different shapes and structures have been reported for different EPS. Li et al [ 38 ] showed that the EPS of Lactobacillus helveticus has rounded to spherical lumps of different sizes. Ahmed et al [ 37 ] found the reticulate shape for the ZW3 EPS.…”

Nutritional characteristics of marine fish Sardinella zunasi Bleeker and immunostimulatory activities of its glycoprotein

“…2, most of the absorption bands could be assigned according to data obtained in previous studies. [50][51][52][53] The broad stretching intense peak at 3356 cm À1 indicated the presence of hydroxyl and amino groups, 54 the 2927 cm À1 region corresponded to the C-H stretching vibration. The band at 1649 cm À1 was the C]O stretching vibration of amide group.…”