Structural characterization and antioxidant activity of purified polysaccharide from cultured Cordyceps militaris

Abstract

The polysaccharide fraction, CM-hs-CPS2, was isolated from fruiting bodies of cultured Cordyceps militaris grown on solid rice medium by hot water extraction and ethanol precipitation, and purified by ion exchange column (DEAE-cellulose-52) and gel filtration column (Sephadex G-100) chromatography. Its structural characterizations were analyzed by gas chromatograph (GC) and fourier transform infrared spectroscopy (FT-IR). Results indicated that CM-hs-CPS2 was a kind of sulfating acidic polysaccharide containing acetamido group. The monosaccharide composition analysis showed that CM-hs-CPS2 was composed mainly of mannose, glucose and galactose in a molar ration of 1.35: 8.34: 1.00, and linked by α-glycosidic linkage. The studies on antioxidant activities of CM-hs-CPS2 were evaluated by various methods in vitro. Results showed that DPPH• scavenging activity, reducing power and ferrous ion chelating activity of CM-hs-CPS2 (8 mg/ml) were 89%, 1.188 and 85%, respectively.

Chemical Activity of the Liquid Cordiceps Militaris Culture From Radix Taraxaci Cum Herba Medium

“…Following the unilateral influence of RTH addition on the soluble dry matter content of the obtained mycelial biomass, it can be seen that the obtained data are statistically assured, registering a significant positive difference compared to the average of experience by 0.24 g soluble dry matter per 100 ml liquid culture medium (Table 2). The results obtained are similar to those presented in the literature for the recipe without the addition of RTH, 2.25 g soluble dry matter / 100 ml liquid culture were determined by Fengyao et al (2011). For the recipe with added RTH no data were found in the literature, but they show significant positive differences for all repetitions performed compared to the recipe without added RTH.…”

Cordyceps militaris: An Overview of Its Chemical Constituents in Relation to Biological Activity

“…Previous studies confirmed the ability of the polysaccharide P70-1 and CBP-1 obtained from C. militaris to eliminate hydroxyl radicals [95,96]. The antioxidant activity and the ability to chelate iron ions (Fe2+) were also proven for the polysaccharide designated as CM-hs-CPS2 [97].…”

Xanthine Oxidase Inhibitory Potential, Antioxidant and Antibacterial Activities of Cordyceps militaris (L.) Link Fruiting Body

“…Obviously, the XO inhibitory activity of C. militaris was attributed to their survival strategy to the oxidative stress. For example, several studies showed that polysaccharides from aqueous extracts of C. militaris possessed antioxidant properties [33,34,35] but there was little polysaccharide quantity found in methanolic extracts [25]. Furthermore, the in vitro antioxidant activity was reported to be correlated to cordycepin [21,52] and fatty acids [53].…”

“…Yong et al [29] found that hot water extract of C. militaris exhibited significant anti-hyperuricemic action but active components for this activity were not determined. Additionally, the investigation on antibacterial performance of aqueous extracts of C. militaris has been proceeded but bioactive compounds from the methanolic extract have not been elaborated [32,33,34,35]. Infectious diseases caused by bacteria are still the major reason of illness and death in developing countries [36].…”