Structural Characterization and Antitumor Activity of Polysaccharides from Kaempferia galanga L.

Abstract

The water-soluble polysaccharides from Kaempferia galanga L. (KGPs) were extracted and purified, and their structural characteristics and antitumor activity were further investigated. The UV spectrum, high-performance gel permeation chromatography (HPGPC), Fourier-transform infrared spectroscopy (FTIR), and ion chromatography (IC) were employed to evaluate the structural characteristics, and H22 tumor-bearing mice model was established to demonstrate the antitumor activity. Physicochemical analysis and UV spectrum results showed that the proportions of total sugar, protein, and uronic acid in KGPs were 85.23%, 0.54%, and 24.17%, respectively. HPGPC, FTIR, and IC indicated that KGPs were acidic polysaccharides with skeletal modes of pyranose rings and mainly composed of arabinose and galactose with the average molecular weight of 8.5 × 105 Da. The in vivo antitumor experiments showed that KGPs could effectively protect the thymus and spleen of tumor-bearing mice from solid tumors and enhance the immunoregulatory ability of CD4+ T cells, the cytotoxic effects of CD8+ T cells and NK cells, and finally resulting in the inhibitory effects on H22 solid tumors. This study provided a theoretical foundation for the practical application of KGPs in food and medical industries.

<p>Protective effects of five compounds from <em>Livistona chinensis</em> R. Brown leaves against hypoxia/reoxygenation, H₂O₂, or adriamycin-induced injury in H9c2 cells</p>

“…The three sets of experiments were performed at standard culture conditions, as follows: 1) untreated control cells; 2) cells were treated with the IC 50 concentration of H 2 O 2 (100 μmol/L) for 4 hrs, and then medium was changed for another 24 hrs; and 3) cells were treated with the IC 50 concentration of H 2 O 2 (100 μM) for 4 hrs, and then the medium was changed. The injured cells were treated with the different concentrations of the five compounds (2.5–40 μg/mL, dissolved in DMSO; Sigma) for 24 hrs 31. Cell viability was analyzed using MTT methods according to the method mentioned above.…”

Bioactive Carbohydrate Polymers—Between Myth and Reality

“…However, the action of polysaccharides is specific for various cancer cells [ 17 , 20 ]. The concentration dependence of the antiproliferative activity of polysaccharides was established [ 14 , 21 , 23 , 49 , 50 ]. A higher degree of branching led to stronger in vitro antitumor activity [ 51 ].…”

A Novel Optimization of Water‐Soluble Compound Polysaccharides from Chinese Herbal Medicines by Quantitative Theory and Study on Its Characterization and Antioxidant Activities

“…The liquor was eluted with NaOH (10 m m ) and NaAc (200 m m ) solutions at a flow rate of 0.5 mL/min, thereby generating a 25 μL sample at a column temperature of 30 °C. Ara, Gal, Glc, Man, Rha, Xyl, Fuc, Rib, Fuc, GalA, and GlcA served as standards [71] …”

“…Ara, Gal, Glc, Man, Rha, Xyl, Fuc, Rib, Fuc, GalA, and GlcA served as standards. [71] FT-IR Spectrometric Analysis…”