Structural characterization and in vivo evaluation of retinyl palmitate in non-ionic lamellar liquid crystalline system

Chorilli, Marlus; Prestes, Paula Souza; Rigon, Roberta Balansin; Leonardi, Gislaine Ricci; Chiavacci, Leila Aparecida; Sarmento, Víctor Hugo Vitorino; Oliveira, Anselmo Gomes de; Scarpa, Maria Virgínia

doi:10.1016/j.colsurfb.2011.02.027

Cited by 62 publications

(52 citation statements)

References 39 publications

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“…The formulation AO3F increased the production of fibroblasts. This, in turn, increased the production of collagen, which is responsible for the strength and resistance of greater thickness and dermal trauma, promoting satisfactory skin restructuring [28,29].…”

Section: Histopathological Analysis Of the Dermismentioning

confidence: 99%

Surfactant-based Transdermal System for Fluconazole Skin Delivery

Silva¹,

Luz²,

Satake³

et al. 2014

J Nanomed Nanotechnol

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The development of a controlled-release dosage form of antifungals is of crucial importance in view of the side-effects of conventional oral and intravenous treatments of Sporotrichosis. In this study, systems composed of polyoxypropylene (5) polyoxyethylene (20) cetyl alcohol (PPG-5-CETETH-20) as a surfactant, oleic acid as an oil phase, and water were developed as a possible fluconazole transdermal drug delivery system. The systems were characterised by polarised light microscopy (PLM), SAXS, and rheological analysis, followed by cellular and histological analyses, in vitro release assays, and ex vivo skin permeation and retention studies using porcine ear tissue and a Franz diffusion cell. PLM and SAXS results indicated that the mixtures of surfactant, oil and water formed micellar and lamellar phases. The incorporation of fluconazole in these systems was greater than in water and conventional dosage forms. Micellar systems behave as Newtonian fluids, being more viscous than elastic in rheological analysis, and lamellar phases behave as pseudoplastic fluids with high elastic moduli. In vitro and in vivo biological assays showed that the formulations did not affect normal cell macrophages and did not promote skin irritation. The release profile indicated that fluconazole could be released in a controlled manner. It was found that the systems enhanced drug permeation and skin retention by changing only the composition of the components in the formulations. Therefore, PPG-5-CETETH-20-based systems have great potential as transdermal systems with different structural and rheological characteristics for Sporotrichosis treatment using antifungal drugs.vehicles. Surfactants are able to stabilise mixtures of components with different polarities, thus creating additional regions for hydrophilic and lipophilic solubilisation of drugs. The drug can be protected against the dissolution medium due to the interfacial membrane, which is composed of the surfactant layer that the drug must pass through to be released. In this manner, surfactant systems also confer reservoir properties. The phase behaviour of surfactants enable the formation of microemulsions and liquid crystals, which is extremely attractive for nanotechnology because these structures are organised on the nanometric scale. In addition to the advantage of increased stability, their production can be scaled up easily. Varying the composition and proportions of surfactant, oil, and aqueous phase, it is possible to control the viscosity and texture of the systems above, which could lead to the immobilisation or localisation of the formulation at specific sites in the body and routes of administration. Therefore, surfactant systems have great potential as transdermal nanostructured drug delivery systems [6].Recently, our research group has demonstrated that the surfactant polyoxypropylene (5)

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Section: Histopathological Analysis Of the Dermismentioning

confidence: 99%

Surfactant-based Transdermal System for Fluconazole Skin Delivery

Silva¹,

Luz²,

Satake³

et al. 2014

J Nanomed Nanotechnol

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“…This technique was applied to identify the hazy systems within the ternary phase diagrams whether they were stable emulsions or LC phases. The anisotropic nature of liquid crystalline system was confirmed under PLM where it showed birefringence due to its molecular organization 39 as shown in Figure 3. As there are some parameters such as drug loading and the alteration of the system components proportions could affect the packing parameter of lecithin, thus affecting the prepared liquid crystalline phase, 60,63 whether drug loading and water capacity would affect the microstructure formed or not was investigated.…”

Section: Polarizing Light Microscope Evaluationmentioning

confidence: 89%

“…39 The structure of the medicated and placebo LCG samples was examined by a polarizing microscope (Model CX 31; Olympus) using a magnification of 100×.…”

Section: Content Uniformitymentioning

confidence: 99%

Novel lecithin-integrated liquid crystalline nanogels for enhanced cutaneous targeting of terconazole: development, in vitro and in vivo studies

Elnaggar

Talaat

Bahey-El-Din

et al. 2016

IJN

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“…MEs are defined as dispersion made of water, oil, and surfactants that is an isotropic and thermodynamically stable system with dispersed in globular or interconnected size diameter varying approximately from 1 to 100 nm, usually 10 to 50 nm (Slomkowski et al, 2011;McClements, 2012). LCS and MEs can increase the physical stability, solubilization, and efficacy of a series of active principles and they can explored to drug or active compounds delivery (Chorilli et al, 2011;Chen, Ma, Gui, 2014;Guo et al, 2010;Lawrence, Rees, 2000). They can be formed by the association of varying concentrations of water, oil, and surfactant and changing the concentrations of surfactant can self-assembly at forms different such as microemulsions or lamellar, hexagonal, and cubic mesophase (Malmsten, 2002;Romsted, 2012).…”

Section: Introductionmentioning

confidence: 99%

“…Liquid crystalline systems (LCS) are mesomorphic phases of matter in an intermediate state in the thermal transformation from a solid to a liquid. LCS have the characteristic properties of solids and liquids: they have the structural order, rigidity, and defined links as in solids, and they also have the mobility, fluidity, and disordered regions as in liquids (Chorilli et al, 2011;Prestes et al, 2009;Mezzenga, 2012). MEs are defined as dispersion made of water, oil, and surfactants that is an isotropic and thermodynamically stable system with dispersed in globular or interconnected size diameter varying approximately from 1 to 100 nm, usually 10 to 50 nm (Slomkowski et al, 2011;McClements, 2012).…”

mentioning

confidence: 99%

Organic cocoa extract -loaded surfactant-based systems intended to skin bioadhesion

Fonseca-Santos

Funagoshi

Oliveira

et al. 2017

Braz. J. Pharm. Sci.

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This study was to develop, characterize, and evaluate the physical-chemical stability, in vitro antioxidant activity and in vitro safety profile of liquid crystalline systems (LCS) and microemulsions (MEs) with and without organic cocoa (OC) extract. LCS stabilized by surfactant polyoxyethylene 20 cetyl ether, containing water and oleic acid were studied. LCS and MEs were characterized using polarized light microscopy, small angle X-ray scattering, rheology and in vitro bioadhesion, and were evaluated for a period of 30 days by visual aspects, centrifuge test, pH value and relative density. PLM and SAXS assays showed the presence of domains of MEs, cubic and hexagonal mesophasephases, varying the proportions of the components of the formulations; where in the addition of the extract did not change rheological behavior of the formulations. All of the formulations were stable in the period analyzed and presented higher bioadhesive strength. In vitro antioxidant activity suggests that LCS and MEs presented a high capacity to maintain the antioxidant activity of OC extract. The results showed that the incorporation of OC in LCS improved the safety profile, according to cytotoxicity assays of systems may be a promising platform to OC extract for topical application for the potential treatment of skin disorders. Keywords:Liquid crystals/evaluation/physical-chemical stability. Microemulsions/evaluation. Surfactant-based system. Organic cocoa extract. Skin bioadhesion. INTRODUCTIONSkin is routinely exposed to stressful environmental factors such as pollutants and UV radiation. These factors produce a large number of aggressive oxidants that damage all of the biological skin cell membranes (Silva et al., 2010;Binic et al., 2013;Datta et al., 2011;Dupont, Gomez, Bilodeau, 2013). Accordingly, the use of skin care products and cosmetics has been utilized to mitigate the cutaneous photoaging process (Visscher, Pan, Kitzmiller, 2013) and these products are commonly composed of plant extracts (Binic et al., 2013).A great number of plants and plant extracts are studied for their antioxidative action arising from their phenolic substructures. Phenolic compounds are composed of an aromatic ring that is linked to a hydroxyl group, which donates electrons and hydrogen atoms. As a result, these compounds are exceptional antioxidants (Silva et al., 2010;Binic et al., 2013;Reuter, Merfort, Schempp, 2010).Cocoa, the dried and fermented seeds derived from Theobroma cacao, has been consumed since 1100 B.C. by ancient civilizations such as the Mayans and Aztecs (Hurst et al., 2002). Cocoa contains high amounts of the flavonoids (−)-epicatechin (EC) and (+)-catechin, and procyanidins B2 and B1. Additionally, other polyphenols such as quercetin, isoquercitrin (quercetin 3-O-glucoside), quercetin 3-O-arabinose, hyperoside (quercetin 3-O-galactoside), naringenin, luteolin, and apigenin have also been found in cocoa in minor quantities (Sánchez-Rabaneda et al., 2003). While the natural amount B. Fonseca-Santos, E. E. Funagoshi, J. R. S. Olivei...

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Structural characterization and in vivo evaluation of retinyl palmitate in non-ionic lamellar liquid crystalline system

Cited by 62 publications

References 39 publications

Surfactant-based Transdermal System for Fluconazole Skin Delivery

Surfactant-based Transdermal System for Fluconazole Skin Delivery

Novel lecithin-integrated liquid crystalline nanogels for enhanced cutaneous targeting of terconazole: development, in vitro and in vivo studies

Organic cocoa extract -loaded surfactant-based systems intended to skin bioadhesion

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