Structural Characterization and Protein Adsorption Property of Hydroxyapatite Particles Modified With Zinc Ions

Abstract

Zinc-containing hydroxyapatite particles (Zn/HAp) were prepared by an ion exchange reaction process involving hydroxyapatite (HAp) particles with aqueous solutions containing various amounts of zinc nitrate. The Zn 21 ion was partially substituted for the Ca 21 ion position in the HAp lattice, and hence, the obtained samples had changed little in crystallinity, particle size, and specific surface area. Adsorption of bovine serum albumin (BSA) and b 2 -microglobulin (b 2 -MG) in solutions containing both BSA and b 2 -MG was examined. As the Zn 21 ion content in the apatites increased, the adsorbed amount of BSA was almost constant, whereas that of b 2 -MG increased.

Substituted hydroxyapatites for biomedical applications: A review

“…Its possible antimicrobial aktivity will be discussed in section 4.2.5. and in section 4.3.1. Zn-HA has been prepared predominantly by a precipitation method [183,[219][220][221][222][223][224][225], but ion-exchange [226], a hydrothermal technique at 200°C [75] and the sol-gel process [188,227] have also been used. Substitution of Zn 2+ into the HA structure through a hydrothermal method is more difficult than through chemical precipitation.…”

Multifunctional and hybrid systems based on calcium-phosphate and hydroxyapatite for preventive and regenerative medicine

“…Sinteza Zn-HAp se pretežno izvodi metodom precipitacije [35][36][37], jonskom izmenom [38], hidrotermalnom sintezom [39], kao i sol-gel procesima [40]. Jonski radijus Zn 2+ jona iznosi 0,074 nm i manji je u poređenju sa Ca 2+ jonskim radijusom koji iznosi 0,099 nm, pa je i očekivano da se parametri rešetke Zn-HAp promene.…”

Quantitative Chemical Mapping of Relevant Trace Elements at Biomaterials/Biological Media Interfaces by Ion Beam Methods

“…The adsorption capacity of a powder is strongly influenced by the pore size and the occupation of the pore by heme proteins can be studied. [58] The usual methods to investigate the protein adsorption on materials surfaces are mainly UV absorption [59,60] and more recently confocal microscopic analysis after labeling the proteins with a fluorescent label. [61] The spectrophotometric method gives information on the total amount of adsorbed proteins but no information on the space distribution of these proteins.…”