Structural Characterization and Tissue-Specific Expression of the MouseGlucose-6-Phosphate DehydrogenaseGene

Abstract

Glucose-6-phosphate dehydrogenase (G6PD) activity differs among tissues and, in liver, with the dietary state of the mouse. Tissue-specific differences in G6PD activity in adipose tissue, liver, kidney, and heart were associated with similar differences in the amount of G6PD mRNA. Regulation of mRNA amount by dietary fat was only observed in liver. In mice fed a low-fat diet, the relative amounts of G6PD mRNA were 3:1:1:0.38, respectively, in the four tissues. Further, the amount of precursor mRNA for G6PD in liver, kidney, and heart reflected the amount of mature mRNA in these tissues, suggesting differing transcriptional activity. Our S1 nuclease and primer-extension analyses indicated that the same transcriptional start site is used in liver, kidney, and adipose tissue, resulting in a common 5' end of the mRNA in these tissues. Thus, differential regulation is not attributable to alternate promoter usage. A DNase hypersensitivity analysis of the 5' end of the G6PD gene identified three hypersensitive sites (HS): HS 1 and HS 2 were present in all tissues, whereas HS 3 was liver specific. Thus, regulation of G6PD expression involves both dietary and tissue-specific signals that appear to act via different mechanisms.

Regulation and properties of glucose-6-phosphate dehydrogenase: A review

“…Three DNase1 hypersensitivity sites (Hss-1, Hss-2 and Hss-3) have been localized in the 5" end of the G6PD gene. Hss-3 located in introns 2 is liver specific whereas the other two Hss-1 and Hss-2 are ubiquitously present in all tissues (Hodge et al, 1998). The translation start site is located in exon 2 and has been mapped in rats and humans (Rank et al, 1994;Franze et al, 1998).…”

Glucose-6-Phosphate Dehydrogenase Deficiency and Malaria: A Method to Detect Primaquine-Induced Hemolysis in vitro

“…The promoter of the G6PD gene contains a TATA-like, TTAAAT sequence, and a great number of stimulatory protein 1 (Sp1) elements (Philippe, et al, 1994, Rank, et al, 1994, Franze, et al, 1998, Hodge, et al, 1998. These Sp1-binding sites are essential for promoter activity (Philippe, et al, 1994).…”