Structural characterization of a novel Schisandra polysaccharides and nutritional intervention in immunotoxicity to PCBs

Zhao, Ting; Wang, Fen; Guo, Yuchao; Ji, Hongchen; Zhang, Weijie; Mao, Guanghua; Feng, Weiwei; Chen, Yao; Yang, Liuqing; Wu, Xiangyang

doi:10.1016/j.carbpol.2020.117380

Cited by 27 publications

(5 citation statements)

References 22 publications

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“…Based on these data, residue A was confirmed to be a T-α-Glcp residue [31]. Similarly, the other monosaccharide residues (B-J) were identified as follows: T-β-Manp (B) [32], T-α-Galp (C) [33], 1,2-α-Manp (D) [19], 1,3-α-Glcp (E) [34], 1,4-α-Glcp (F) [35], 1,6-β-Glcp (G) [36], 1,3,4-α-Glcp (H) [37], 1,4,6-α-Glcp (I) [35], and 1,4,6-α-Galp (J) [38]. The residues A-J were further structurally assigned and sequenced based on key HMBC correlations (Figure 2e).…”

Section: Structural Characterization Of Asp-1mentioning

confidence: 82%

A New Exopolysaccharide of Marine Coral-Associated Aspergillus pseudoglaucus SCAU265: Structural Characterization and Immunomodulatory Activity

Peng,

Liu,

Lin

et al. 2023

JoF

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Recent studies have found that many marine microbial polysaccharides exhibit distinct immune activity. However, there is a relative scarcity of research on the immunomodulatory activity of marine fungal exopolysaccharides. A novel water-soluble fungal exopolysaccharide ASP-1 was isolated from the fermentation broths of marine coral-associated fungus Aspergillus pseudoglaucus SCAU265, and purified by Diethylaminoethyl-Sepharose-52 (DEAE-52) Fast Flow and Sephadex G-75. Structural analysis revealed that ASP-1 had an average molecular weight of 36.07 kDa and was mainly composed of (1→4)-linked α-D-glucopyranosyl residues, along with highly branched heteropolysaccharide regions containing 1,4,6-glucopyranosyl, 1,3,4-glucopyranosyl, 1,4,6-galactopyranosyl, T(terminal)-glucopyranosyl, T-mannopyranosyl, and T-galactopyranosyl residues. ASP-1 demonstrated significant effects on the proliferation, nitric oxide levels, and the secretion of cytokines TNF-α and IL-6 in macrophage RAW264.7 cells. Metabolomic analysis provided insights into the potential mechanisms of the immune regulation of ASP-1, suggesting its involvement in regulating immune function by modulating amino acid anabolism, particularly arginine synthesis and metabolism. These findings provide fundamental scientific data for further research on its accurate molecular mechanism of immunomodulatory activity.

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Section: Structural Characterization Of Asp-1mentioning

confidence: 82%

A New Exopolysaccharide of Marine Coral-Associated Aspergillus pseudoglaucus SCAU265: Structural Characterization and Immunomodulatory Activity

Peng,

Liu,

Lin

et al. 2023

JoF

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“…The carbon chemical shifts of rEPS‐2 were similar to those of β‐(1,2)‐glucans. Moreover, polysaccharide rEPS‐2 included mannose, glucose, and galactose, with molar ratios of 0.38:0.30:0.32, and the terminals consisted of β‐ d ‐Man‐(1 → and α‐ d ‐Galp‐(1→, respectively 30,32,33 . It can thus be speculated that the structure is a main chain containing three residues (→3)‐β‐ d ‐Glcp‐(1 → 4,6)‐β‐ d ‐Manp‐(1→; →2)‐β‐ d ‐Glcp‐(1 → 2,3)‐α‐ d ‐Galp‐(1→; α‐ d ‐Galp‐(1→) which has a side chain containing two residues (β‐ d ‐Manp‐(1 → 4)‐β‐ d ‐Man‐(1 → 6 and α‐ d ‐Manp‐(1 → 4)‐β‐ d ‐Man‐(1) (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Moreover, polysaccharide rEPS-2 included mannose, glucose, and galactose, with molar ratios of 0.38:0.30:0.32, and the terminals consisted of ⊎-D-Man-(1 → and ⊍-D-Galp-(1→, respectively. 30,32,33 It can thus be speculated that the structure is a main chain containing three residues (→3)-⊎-D-Glcp-( 1…”

Section: Nmr Spectrummentioning

confidence: 99%

Structural characterization and hepatoprotective activity of exopolysaccharide from Bacillus velezensisSN‐1

et al. 2022

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BACKGROUND: Exopolysaccharide biopolymers produced by microorganisms are crucial to the environment. They contribute to areas such as the health and bionanotechnology sectors, food and cosmetic industries as gelling agents, and environmental sector as flocculants owing to their biodegradability and non-toxic nature. The current study aimed to isolate the fraction of released exopolysaccharide (rEPS) by Bacillus velezensis SN-1 from Chinese Da-Jiang. RESULTS:The weighted average molecular weight of the major isolated component, rEPS-2, was 202 kDa, and its monosaccharide composition included mannose, glucose, and galactose at a molar ratio of 0.38:0.30:0.32. Further, the rEPS-2 was characterized using methylation analysis and one-dimensional/two-dimensional nuclear magnetic resonance (1D/2D NMR) spectroscopy. In vivo hepatoprotective effects indicated that rEPS-2 could alleviate carbon tetrachloride (CCl 4 )-induced liver injury in mice by lowering the activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and the malondialdehyde (MDA) levels. Furthermore, rEPS-2 can increase the expression of antioxidant genes HO-1, GCLC and NQO1 in the Nrf2/ARE signaling pathway, thereby increasing the activity of antioxidant enzymes superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and reduced catalase (CAT) in liver cells. Furthermore, the rEPS-2 can be used and modulate the gut microbiota of mice with liver injury caused by CCl 4 .CONCLUSIONS: These results suggest that rEPS-2 has promising potential to serve as hepatoprotective agents.

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“…Zhao et al isolated a new polysaccharide SCPP22, with a molecular weight of 143 ± 0.13 kDa from S. chinensis fruit by HPLC, GC–MS, and NMR. SCPP22 is mainly composed of glucose and galactose ( 120 ). Researchers further investigated the function of this polysaccharide and found that SCPP22 may reverse polychlorinated biphenyl (PCB126)-induced immunosuppression by regulating the expression of apoptosis-related proteins, and increase the level of SOD activity and reduce malondialdehyde (MDA) in the spleen and thymus, thus ameliorating oxidative damage in immune organs ( 120 ).…”

Section: Nutritional and Active Components Of Schisandra Ch...mentioning

confidence: 99%

“…SCPP22 is mainly composed of glucose and galactose ( 120 ). Researchers further investigated the function of this polysaccharide and found that SCPP22 may reverse polychlorinated biphenyl (PCB126)-induced immunosuppression by regulating the expression of apoptosis-related proteins, and increase the level of SOD activity and reduce malondialdehyde (MDA) in the spleen and thymus, thus ameliorating oxidative damage in immune organs ( 120 ). Polychlorinated biphenyls (PCBs) are ubiquitous environmental contaminants, which can attack the immune and nervous systems after ingesting by people ( 121 ).…”

Section: Nutritional and Active Components Of Schisandra Ch...mentioning

confidence: 99%

An analysis of the nutritional effects of Schisandra chinensis components based on mass spectrometry technology

Jia¹,

Li²,

Lou³

et al. 2023

Front. Nutr.

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ObjectiveSchisandra chinensis (Turcz.) Baill. (S. chinensis) is a Traditional Chinese medicinal herb that can be used both for medicinal purposes and as a food ingredient due to its beneficial properties, and it is enriched with a wide of natural plant nutrients, including flavonoids, phenolic acids, anthocyanins, lignans, triterpenes, organic acids, and sugars. At present, there is lack of comprehensive study or systemic characterization of nutritional and active ingredients of S. chinensis using innovative mass spectrometry techniques.MethodsThe comprehensive review was conducted by searching the PubMed databases for relevant literature of various mass spectrometry techniques employed in the analysis of nutritional components in S. chinensis, as well as their main nutritional effects. The literature search covered the past 5 years until March 15, 2023.ResultsThe potential nutritional effects of S. chinensis are discussed, including its ability to enhance immunity, function as an antioxidant, anti-allergen, antidepressant, and anti-anxiety agent, as well as its ability to act as a sedative-hypnotic and improve memory, cognitive function, and metabolic imbalances. Meanwhile, the use of advanced mass spectrometry detection technologies have the potential to enable the discovery of new nutritional components of S. chinensis, and to verify the effects of different extraction methods on these components. The contents of anthocyanins, lignans, organic acids, and polysaccharides, the main nutritional components in S. chinensis, are also closely associated to its quality.ConclusionThis review will provide guidelines for an in-depth study on the nutritional value of S. chinensis and for the development of healthy food products with effective components.

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Structural characterization of a novel Schisandra polysaccharides and nutritional intervention in immunotoxicity to PCBs

Cited by 27 publications

References 22 publications

A New Exopolysaccharide of Marine Coral-Associated Aspergillus pseudoglaucus SCAU265: Structural Characterization and Immunomodulatory Activity

A New Exopolysaccharide of Marine Coral-Associated Aspergillus pseudoglaucus SCAU265: Structural Characterization and Immunomodulatory Activity

Structural characterization and hepatoprotective activity of exopolysaccharide from Bacillus velezensisSN‐1

An analysis of the nutritional effects of Schisandra chinensis components based on mass spectrometry technology

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