Structural characterization of a polysaccharide from Lycium barbarum and its neuroprotective effect against β-amyloid peptide neurotoxicity

Wu, Jiaxin; Chen, Teng; Wan, Fengqi; Wang, Jie; Li, Xin; Li, Wenjian; Ma, Liang

doi:10.1016/j.ijbiomac.2021.02.016

Cited by 38 publications

(13 citation statements)

References 47 publications

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“…Residue E was found to have a downfield H-1 (5.04 ppm) and a downfield C-1 (98.7 ppm), consistent with →3,4)- α -D-Glc p -(1→ [ 33 ]. Residue K had an anomeric proton signal and a carbon signal at 4.52 ppm and 104.69 ppm, which were assigned to →3)- β -D-Gal p -(1→ [ 34 ]. The anomeric signals at δ H /δ C 4.99/100.39 and 4.56/105.74 were attributed to →4)- α -D-GalA p -(1→ (residue F) and β -D-Gal p -(1→ (residue J), respectively [ 35 , 36 ].…”

Section: Resultsmentioning

confidence: 99%

Isolation, Structural Characterization and Macrophage Activation Activity of an Acidic Polysaccharide from Raspberry Pulp

et al. 2022

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The discovery of safe and effective plant polysaccharides with immunomodulatory effects has become a research hotspot. Raspberry is an essential commercial fruit and is widely distributed, cultivated, and consumed worldwide. In the present study, a homogeneous acidic polysaccharide (RPP-2a), with a weight-average molecular weight (Mw) of 55582 Da, was isolated from the pulp of raspberries through DEAE-Sepharose Fast Flow and Sephadex G-200 chromatography. RPP-2a consisted of rhamnose, arabinose, galactose, glucose, xylose, galacturonic acid and glucuronic acid, with a molar ratio of 15.4:9.6:7.6:3.2:9.1:54.3:0.8. The results of Fourier transform infrared spectroscopy (FT-IR), gas chromatography-mass spectrometer (GC-MS), 1D-, and 2D-nuclear magnetic resonance (NMR) analyses suggested that the backbone of RPP-2a was primarily composed of →2)-α-L-Rhap-(1→, →2,4)-α-L-Rhap-(1→, →4)-α-D-GalAp-(1→, and →3,4)-α-D-Glcp-(1→ sugar moieties, with side chains of α-L-Araf-(1→, α-L-Arap-(1→, and β-D-Galp-(1→3)-β-D-Galp-(1→ residues linked to the O-4 band of rhamnose and O-3 band of glucose residues. Furthermore, RPP-2a exhibited significant macrophage activation activity by increasing the production of nitric oxide (NO), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1β (IL-1β), and the expression of inducible nitric oxide synthase (iNOS) and cytokines at the transcriptional level in RAW264.7 cells. Overall, the results indicate that RPP-2a can be utilized as a potential natural immune-enhancing agent.

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Section: Resultsmentioning

confidence: 99%

Isolation, Structural Characterization and Macrophage Activation Activity of an Acidic Polysaccharide from Raspberry Pulp

et al. 2022

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“…Combined with monosaccharide composition, FT‐IR, Methylation, NMR analysis and previous studies, it can be inferred that LCFP‐3 from Ilex cornuta fruits may be composed of residues, T ‐ α ‐Arap, T ‐ α ‐Rhap, 1,3‐ α ‐Arap, 1,4‐ α ‐Arap, T‐ β ‐Galp, 1,4‐ α ‐GalpA( O Me), 1,4‐ β ‐Glcp, 1,3‐ β ‐Galp, 1,3,6‐ α ‐Manp, 1,6‐ β ‐Galp, 1,3,4‐ β ‐GalpA, 1,4,6‐ β ‐Manp, 1,3,6‐ β ‐Glcp, 1,2,3,4‐ α ‐Xylp [30–34] …”

Section: Resultsmentioning

confidence: 73%

“…[29] Fourteen cross peaks were found in the anomeric region of HSQC spectrum (Figure 6c Combined with monosaccharide composition, FT-IR, Methylation, NMR analysis and previous studies, it can be inferred that LCFP-3 from Ilex cornuta fruits may be composed of residues, T-α-Arap, T-α-Rhap, 1,3α-Arap, 1,4-α-Arap, T-β-Galp, 1,4-α-GalpA(OMe), 1,4-β-Glcp, 1,3-β-Galp, 1,3,6-α-Manp, 1,6-β-Galp, 1,3,4-β-GalpA, 1,4,6-β-Manp, 1,3,6-β-Glcp, 1,2,3,4-α-Xylp. [30][31][32][33][34]…”

Section: Nmr Analysis Of Lcfp-3mentioning

confidence: 99%

Isolation, Structural Analysis and Anti‐Inflammatory Activity of a Polysaccharide from Ilex cornuta Fruits

Cui

Tian

Sun

et al. 2022

Chemistry & Biodiversity

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In the present study, a polysaccharide from Ilex cornuta fruits (LCFP‐3) was obtained by hot water extraction, Diethyaminoethyl cellulose‐52 (DEAE‐52) chromatography column and Sephadex G‐100 gel column purification. Its structural characteristics were further explored using high performance anion exchange chromatography (HPAEC), gas chromatography and mass spectrometry (GC/MS), scanning electron microscopy (SEM), Fourier transform infrared (FT‐IR) spectroscopy and nuclear magnetic resonance (NMR) spectroscopy. Monosaccharide composition analysis revealed LCFP‐3 contained mainly Galactose (31.92 %), Arabinose (25.87 %) and Galacturonic acid (23.35 %) while small percentage of Rhamnose, Glucose, Mannose and Xylose. Chemical composition analysis showed that the total sugar content of LCFP‐3 was 90.31 % and the protein content was 0.246 %. Gel permeation chromatography (GPC) analysis showed that its average molecular weight was 41.199 kDa. Structural analysis showed that LCFP‐3 may be composed of residues, T‐α‐Arap, T‐α‐Rhap, 1,3‐α‐Arap, 1,4‐α‐Arap, T‐β‐Galp, 1,4‐α‐GalpA(OMe), 1,4‐β‐Glcp, 1,3‐β‐Galp, 1,3,6‐β‐Manp, 1,6‐β‐Galp, 1,3,4‐β‐GalpA, 1,4,6‐β‐Manp, 1,3,6‐β‐Glcp, 1,2,3,4‐α‐Xylp. The anti‐inflammatory activity of LCFP‐3 was evaluated using lipopolysaccharide (LPS)‐induced RAW246.7 macrophages. The results showed that 1–200 μg/mL LCFP‐3 could dose‐dependently protect against LPS‐induced toxicity and 1 μg/mL LCFP‐3 could significantly inhibit LPS‐induced NO production. Therefore, LCFP‐3 exerted an anti‐inflammatory activity and has great potential as a functional ingredient.

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“…It has been mentioned that LBP-1, LBP-2, LBP-3, LBP-4, and LBP-5 fractions were purified from the crude LBP, and the structure features were investigated in our recent study [ 51 ]. In order to find out which fraction of LBP is mainly responsible for preventing DN and the detailed mechanism of it, purified LBP will be used in future research, expecting to provide more scientific data on its pharmacological activity.…”

Section: Discussionmentioning

confidence: 99%

Effect of Lycium barbarum Polysaccharide on Decreasing Serum Amyloid A3 Expression through Inhibiting NF-κB Activation in a Mouse Model of Diabetic Nephropathy

Wan

et al. 2022

Analytical Cellular Pathology

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Lycium barbarum polysaccharide (LBP) as one of the main bioactive constituents of the fruit of Lycium barbarum L. (LBL.) has many pharmacological activities, but its antihyperglycemic activity is not fully understood yet. This study investigated the hypoglycemic and renal protective effects of LBP on high-fat diet/streptozotocin- (HFD/STZ-) induced diabetic nephropathy (DN) in mice. Blood glucose was assessed before and after 8-week administration of LBP, and the homeostasis model assessment-insulin resistance (HOMA-IR) index was calculated for evaluating the antidiabetic effect of LBP. Additionally, serum creatinine (sCr), blood urea nitrogen (BUN), and urine microalbumin were tested to evaluate the renal function. HE and PAS stainings were performed to evaluate the morphology and injury of the kidney. The results showed that LBP significantly reduces the glucose level and ameliorates the insulin resistance of diabetic mice. Importantly, LBP improves renal function by lowering the levels of sCr, BUN, and microalbumin in diabetic mice and relieves the injury in the renal glomeruli and tubules of the DN mice. Furthermore, LBP attenuates renal inflammation as evidenced by downregulating the mRNA levels of TNFα, IL1 β, IL6, and SAA3 in the renal cortex, as well as reducing the elevated circulating level and protein depositions of SAA3 in the kidney. In addition, our western blot results showed that NF-κB p65 nuclear translocation and the degradation of inhibitory κB-α (IκBα) occurred during the progress of inflammation, and such activated signaling was restrained by LBP. In conclusion, our findings suggest that LBP is a potential antidiabetic agent, which ameliorates the inflammation in DN through inhibiting NF-κB activation.

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Structural characterization of a polysaccharide from Lycium barbarum and its neuroprotective effect against β-amyloid peptide neurotoxicity

Cited by 38 publications

References 47 publications

Isolation, Structural Characterization and Macrophage Activation Activity of an Acidic Polysaccharide from Raspberry Pulp

Isolation, Structural Characterization and Macrophage Activation Activity of an Acidic Polysaccharide from Raspberry Pulp

Isolation, Structural Analysis and Anti‐Inflammatory Activity of a Polysaccharide from Ilex cornuta Fruits

Effect of Lycium barbarum Polysaccharide on Decreasing Serum Amyloid A3 Expression through Inhibiting NF-κB Activation in a Mouse Model of Diabetic Nephropathy

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