Structural characterization of a polysaccharide isolated from Lady Godiva pumpkins (Cucurbita pepo lady godiva)

Abstract

In this study, a crude water-soluble heteropolysaccharide was isolated from Lady Godiva pumpkins (Cucurbita pepo Lady Godiva). After protein removal and purification, polysaccharides of the pumpkin fruit termed LGPP2-1 were subjected to structural identification. Gas chromatography-mass spectrometry (GC-MS) analysis indicated that LGPP2-1 mainly comprised L-fucose, D-galactose, D-glucose, and D-mannose. The glycosidic linkages were determined by methylation analysis and GC/MS. The results showed that LGPP2-1 consisted of 1,2,6-trisubstitued-galactopyranosyl, 1,6-disubstitued-galactopyranosyl, 1,4,6-trisubstitued-glucopyranosyl, 1,3-disubstituedglucopyranosyl, terminal-glucopyranosyl, and terminal-fucopyranosyl, with a molar proportion of 1:4:2:2:2:1. The Nuclear magnetic resonance (NMR) spectroscopy further confirmed that the configuration of L-fucose and D-galactose were in the α-form, while D-glucose was in the β-form. The molecular weight of LGPP2-1 measured using high-performance gel-permeation chromatography (HPGPC) was 1.01×10 4 Da.

Effect of phosphorylation on antioxidant activities of pumpkin ( Cucurbita pepo , Lady godiva) polysaccharide

“…Pumpkin polysaccharides were extracted by hot water following the method of Song et al [14]. Briefly, the pumpkins were first treated with water of 85 • C, and then extracted with anhydrous ethanol to the final alcohol concentration of 80%.…”

Characterization and bioactivities of a novel purified polysaccharide from Endothelium corneum gigeriae Galli

“…The excess acid was evaporated by co-distillation with distilled water [26]. The hydrolyzed product was reduced with NaBH 4 for 24 h, and acetylated with acetic anhydride-pyridine (1:1, v/v) at 100 • C for 2 h [27]. 2.4.6.…”

Solution properties of a heteropolysaccharide extracted from pumpkin ( Cucurbita pepo , lady godiva)

“…Pumpkin polysaccharides LGPP2-2 were extracted by hot water following the method of Song et al (2011). Briefly, the fresh pumpkins were pulped using the beater after peeling.…”

“…Kraushofer, & Sontag, 2004). In our previous work, the structure and hypoglycemic activity of pumpkin polysaccharide LGPP2-1 (Song, Li, Hu, Ni, & Li, 2011;Li et al, 2005) had been studied.…”