Structural characterization of a sulfated polysaccharide from Gracilariopsis lemaneiformis and its potentiation of cisplatin antitumor activity in Colon-26 carcinoma tumor-bearing mice by inducing ferroptosis

Abstract

Ferroptosis, a form of regulated cell death caused by iron-mediated lipid peroxidation, has become a potential strategy to overcome drug resistance and improve the efficacy of traditional cancer treatments. In...

Administration of Gracilariopsis lemaneiformis polysaccharide attenuates cisplatin‐induced inflammation and intestinal mucosal damage in colon‐26 carcinoma tumor‐bearing mice

“…Our previous study illustrated that GP90 is a sulfate-rich polysaccharide that is composed mainly of (1→3)-linked 4-O-sulfate-⊎-D-galactose and (1→4)-linked 3,6-anhydro-⊍-L-galactose with a molecular weight of 12.45 kDa. 13 Previous studies have demonstrated that sulfate groups can serve as electrophilic groups, thereby augmenting the activity of GP. The activity of GP is intricately linked to the position and quantity of sulfate groups present on the polysaccharide backbone.…”

“…12 In our previous investigation, it was observed that GP90, a sulfated polysaccharide derived from G. lemaneiformis, has the potential to reduce tumor growth in colon-26 carcinoma tumorbearing mice and enhance sensitivity to cisplatin. 13 These findings suggest that GP90 could have a positive impact on cisplatin therapy efficacy. Therefore, the present study aimed to explore the protective effects of GP90 on systemic inflammation and intestinal barrier damage caused by cisplatin treatment in C26 tumor-bearing mice.…”

“…Previous studies have demonstrated that GP90 exerts potential antitumor effects, enhances the sensitivity of tumor cells to cisplatin and has non-toxic effects. 13 The effect of GP90 on the immune/inflammatory response of tumor-bearing mice is shown in Fig. 2.…”

“…These findings are consistent with previous studies demonstrating that GP90 enhances the tumor inhibition rate of cisplatin. 13 A decrease in the CD4 + /CD8 + T cell ratio can impair the body's immune function, thereby reducing the ability of the immune system to recognize and eliminate abnormal cells and enabling rapid tumor growth, metastasis and secondary infections. 17 Therefore, GP90 effectively reverses the decrease in the CD4 + /CD8 + T cell ratio caused by cisplatin and effectively regulates the body's inflammatory immune response.…”

Ferroptosis: Potential opportunities for natural products in cancer therapy

“…Red ginseng polysaccharide (RGP) inhibited the proliferation of A549 and MDA‐MB‐231 cells by reducing the expression of GPX4, whereas ferrostatin‐1 counteracted the effects of RGP, suggesting that this polysaccharide could inhibit tumor development by regulating ferroptosis (Zhai et al, 2022). Cai et al isolated a new polysaccharide GP90 from Gracilariopsis lemaneiformis , which was composed of galactose and anhydro‐galactose (B. Cai, Luo, et al, 2023). Importantly, this polysaccharide promoted ferroptosis in C26 cells through the down‐regulation of SLC7A11 and GPX4, thus significantly attenuating cisplatin resistance in CRC.…”