Structural Characterization of Peptides That Bind Synovial Fluid Antibodies from RA Patients: A Novel Strategy for Identification of Disease-Related Epitopes Using a Random Peptide Library

Unravelling autoimmune pathogenesis by screening random peptide libraries with human sera

“…First attempts were tried to verify the applicability of RPL technology to the study of the etiopathogenesis of human autoimmunity with special reference to RA [66], multiple sclerosis (MS) [67], vitiligo [68], male immunoinfertility [69]. In these initial experiments a number of disease-related phagotopes were selected by human sera, though not ultimately characterised.…”

Epitope Discovery Using Bacteriophage Display: The Minimum Epitope of an Anti-IRBP Antibody

“…Random peptide screening, therefore, has practical applications in identifying retinal antigen epitopes recognized by antibodies generated in vivo, in patients suffering from ocular immune-mediated diseases such as (auto)immune uveitis or corneal graft rejection. Analysis of natural antibody specificity has already yielded useful experimental, diagnostic and potentially therapeutic epitope mimetics in other disease states such as rheumatoid arthritis (Dybwad et al, 1995) and numerous infectious diseases (Meola et al, 1995 ;Stoute et al, 1995 ;Lundin et al, 1996).…”

Importin beta: A novel autoantigen in human autoimmunity identified by screening random peptide libraries on phage

“…The technology was originally applied for screening RPLs with sera from individuals immunised against hepatitis B and C surface antigens [6e10]. The same technology has been employed also to study human autoimmune diseases, including rheumatoid arthritis [11], multiple sclerosis [12,13], autoimmune thrombocytopenia purpura [14] and autoimmune inner ear disorders, such as Cogan's syndrome [15], resulting in the selection of a number of disease-related epitopes (mimotopes). The same experimental approach was applied to type II cryoglobulinemia, which led to the identification of mimotopes of the CD4-like LAG-3 protein [16] and to primary biliary cirrhosis (PBC), where phagotopes, corresponding to an immunodominant epitope of pyruvate dehydrogenases complex, were identified [17].…”