Structural Characterization of Set1 RNA Recognition Motifs and their Role in Histone H3 Lysine 4 Methylation

Tresaugues, L.; Dehé, Pierre-Marie; Guérois, Raphaël; Rodríguez-Gil, Alfonso; Varlet, Isabelle; Salah, Philippe; Pamblanco, Mercè; Luciano, Pierre; Quevillon‐Chéruel, Sophie; Sollier, Julie; Leulliot, Nicolas; Couprie, Joël; Tordera, Vicente; Zinn‐Justin, Sophie; Chávez, Sebastián; Tilbeurgh, Herman van; Géli, Vincent

doi:10.1016/j.jmb.2006.04.050

Cited by 53 publications

(71 citation statements)

References 49 publications

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“…A role for the N-SET domain in the regulation of epigenetic writing was recently assigned to yeast Set1 (Kim et al, 2013). Also, RNA binding by the RRM domain, which conceptually presents great potential for regulatory specificity, has been described for yeast Set1 (Trésaugues et al, 2006). Differences between the functions of the N-SET or RRM domains in Setd1a and Setd1b, in addition to specific protein-protein interactions mediated by the divergent sections of these very similar proteins, are likely to be the source of their different roles in mouse development.…”

Section: Discussionmentioning

confidence: 99%

The H3K4 methyltransferase Setd1a is first required at the epiblast stage, whereas Setd1b becomes essential after gastrulation

et al. 2014

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Histone 3 lysine 4 (H3K4) methylation is a universal epigenetic mark. In mammals, there are six H3K4 methyltransferases related to yeast Set1 and fly Trithorax, including two orthologs of Set1: Setd1a and Setd1b. Here we show that mouse Setd1a is required for gastrulation, whereas Setd1b-deficient embryos survive to E11.5 but are grossly retarded. Setd1a knockout embryos implant but do not proceed past the epiblast. Furthermore, Setd1a is not required until the inner cell mass has formed, at which stage it has replaced Mll2 as the major H3K4 methyltransferase. Setd1a is required for embryonic, epiblast and neural stem cell survival and neural stem cell reprogramming, whereas Setd1b is dispensable. Deletion of Setd1a in embryonic stem cells resulted in rapid losses of bulk H3K4 methylation, pluripotency gene expression and proliferation, with G1 pileup. Setd1b overexpression could not rescue the proliferation defects caused by loss of Setd1a in embryonic stem cells. The precise developmental requirement for Setd1a suggests that gastrulation is regulated by a switch between the major H3K4 methyltransferases.

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Section: Discussionmentioning

confidence: 99%

The H3K4 methyltransferase Setd1a is first required at the epiblast stage, whereas Setd1b becomes essential after gastrulation

et al. 2014

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“…Set1 contains two RNA Recognition Motifs (RRM1 and RRM2), which could be required to recognize antisense RNAs and induce silencing. To test this possibility, the Dset1 strain containing plasmid N°1 was transformed with SET1 plasmids in which the RRM1 or RRM2 motifs were mutated (Tresaugues et al 2006). These Set1 mutants were still able to restore silencing, indicating that the RNA recognition motifs of Set1 are not implicated (Fig.…”

Section: Set1 Influences the Silencing In Trans By Promoting Productimentioning

confidence: 99%

Trans-acting antisense RNAs mediate transcriptional gene cosuppression in S. cerevisiae

Camblong¹,

Beyrouthy²,

Guffanti³

et al. 2009

Genes Dev.

143

138

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Homology-dependent gene silencing, a phenomenon described as cosuppression in plants, depends on siRNAs. We provide evidence that in Saccharomyces cerevisiae, which is missing the RNAi machinery, protein coding gene cosuppression exists. Indeed, introduction of an additional copy of PHO84 on a plasmid or within the genome results in the cosilencing of both the transgene and the endogenous gene. This repression is transcriptional and position-independent and requires trans-acting antisense RNAs. Antisense RNAs induce transcriptional gene silencing both in cis and in trans, and the two pathways differ by the implication of the Hda1/2/3 complex. We also show that trans-silencing is influenced by the Set1 histone methyltransferase, which promotes antisense RNA production. Finally we show that although antisense-mediated cis-silencing occurs in other genes, transsilencing so far depends on features specific to PHO84. All together our data highlight the importance of noncoding RNAs in mediating RNAi-independent transcriptional gene silencing.[Keywords: Antisense RNA; cis and trans transcriptional gene silencing; PHO84; cosuppression; RNAi-independent TGS; noncoding RNA; S. cerevisiae] Supplemental material is available at http://www.genesdev.org.

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“…In addition to the role of H3K4 methylation in transcription, SET1 deletion causes pleiotropic defects in telomere length, DNA repair, chromosome segregation, and meiotic differentiation (8). The presence of two predicted RNA recognition motifs in Set1, one of which has been shown to bind RNA in vitro, also raises the possibility of COMPASS interacting with RNA (10,11).…”

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confidence: 99%

Yeast Swd2 Is Essential Because of Antagonism between Set1 Histone Methyltransferase Complex and APT (Associated with Pta1) Termination Factor

Soares¹,

Buratowski

2012

Journal of Biological Chemistry

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Structural Characterization of Set1 RNA Recognition Motifs and their Role in Histone H3 Lysine 4 Methylation

Cited by 53 publications

References 49 publications

The H3K4 methyltransferase Setd1a is first required at the epiblast stage, whereas Setd1b becomes essential after gastrulation

The H3K4 methyltransferase Setd1a is first required at the epiblast stage, whereas Setd1b becomes essential after gastrulation

Trans-acting antisense RNAs mediate transcriptional gene cosuppression in S. cerevisiae

Yeast Swd2 Is Essential Because of Antagonism between Set1 Histone Methyltransferase Complex and APT (Associated with Pta1) Termination Factor

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