Structural characterization of Tn<i>916</i>-like element in<i>Streptococcus parauberis</i>serotype II strains isolated from diseased Japanese flounder

Meng, Fei; Kanai, Kazuaki; Yoshikoshi, Kazuma

doi:10.1111/j.1472-765x.2009.02609.x

Cited by 5 publications

(5 citation statements)

References 21 publications

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“…In this study, tet (M) was detected in 60% of the II isolates and only in 1.6% and 0% of the Ia and Ib/Ic isolates respectively. Interestingly, 95.2% of the Ia isolates contained tet (S), which is similar to the findings of a previously published report (Meng et al, 2009). The mechanism of tetracycline resistance mediated by the tet (M) and tet (S) genes are similar ways, because tet (S) has 78% level of amino acid sequence identity to the tet (M) gene (Roberts, 1996).…”

Section: Discussionsupporting

confidence: 91%

Prevalence, antibiotic susceptibility and serotyping of Streptococcus parauberis isolates from diseased marine fish

et al. 2021

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Streptococcus parauberis is the main causative bacterial pathogen for streptococcosis in marine fish, causing a significant economic loss to the mariculture industry. In this study, we aimed to investigate the serotypes and antimicrobial susceptibilities of 83 S. parauberis isolates from diseased farmed fish (Paralichthys olivaceus, Platichthys stellatus and Sebastes schlegelii) in Korea. This study categorized the S. parauberis isolates based on PCR serotyping into serotype I (subtypes Ia and Ib/Ic) and serotype II. Serotype Ia was identified to be significantly more prevalent than serotypes Ib/Ic and II. An antimicrobial susceptibility analysis showed that all of the isolates were susceptible to amoxicillin/clavulanic acid. Overall, the isolates studied showed resistance to penicillin and ampicillin (1.2%), tetracycline (68.7%), oxytetracycline (72.3%), doxycycline (53.0%) and erythromycin (37.3%). Interestingly, we found that serotypes Ia and II showed higher degrees of tetracycline resistance than serotype Ib/Ic. The macrolide resistance gene erm(B) was present in 75.8% of the Ia isolates, whereas the mef(A) gene was not detected in any of the isolates. The tetracycline resistance genes tet(S) and tet(M) were detected in 95.2% and 60% of Ia and II isolates respectively. Moreover, β‐lactam antibiotic‐resistant Ia isolates contained a set of 11 mutations in PBP1A and PBP2X. Our study provides a valuable reference for surveillance of antimicrobial resistance and underscores the strict management of antibiotic use to prevent the emergence of resistant of S. parauberis serotypes.

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Section: Discussionsupporting

confidence: 91%

Prevalence, antibiotic susceptibility and serotyping of Streptococcus parauberis isolates from diseased marine fish

et al. 2021

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“…Except for elements from the Tn916-Tn1545 family which were broadly reported in different streptococcal species originating from diverse animal hosts (9,94,170,196), ICEs carrying resistance genes have rarely been reported in streptococci from animal origin. Indeed, only the mosaic tet(O/W/32/O) carried on ICESsu32457 in S. suis, which could then be transferred to S. pneumoniae, S. pyogenes, and S. agalactiae (197), as well as the lincomycin-resistance gene lsa (192) such MGEs.…”

Section: Role Of the Ices In The Evolution Of Resistancementioning

confidence: 99%

Antimicrobial Resistance inStreptococcusspp

2018

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The genus includes Gram-positive organisms shaped in cocci and organized in chains. They are commensals, pathogens, and opportunistic pathogens for humans and animals. Most species of veterinary relevance have a specific ecological niche, such as , which is almost exclusively an environmental pathogen causing bovine mastitis. In contrast, can be considered as a true zoonotic pathogen, causing specific diseases in humans after contact with infected animals or derived food products. Finally, species such as can be sporadically zoonotic, even though they are pathogens of both humans and animals independently. For clarification, a short taxonomical overview will be given here to highlight the diversity of streptococci that infect animals. Several families of antibiotics are used to treat animals for streptococcal infections. First-line treatments are penicillins (alone or in combination with aminoglycosides), macrolides and lincosamides, fluoroquinolones, and tetracyclines. Because of the selecting role of antibiotics, resistance phenotypes have been reported in streptococci isolated from animals worldwide. Globally, the dynamic of resistance acquisition in streptococci is slower than what is experienced in , probably due to the much more limited horizontal spread of resistance genes. Nonetheless, transposons or integrative and conjugative elements can disseminate resistance determinants among streptococci. Besides providing key elements on the prevalence of resistance in streptococci from animals, this article will also largely consider the mechanisms and molecular epidemiology of the major types of resistance to antimicrobials encountered in the most important streptococcal species in veterinary medicine.

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“…Meng et al (2009a) found that a serotype II S. parauberis strain isolated from olive ounder contained both Tn916-like elements encoding tet(M). Similarly, Meng et al (2009b) found that all serotype II strains carried tet(M) and both integrase (int) and excisase (xis) genes were detected, suggesting the presence of a Tn916-like element. In this study, serotype II was represented by 21 of 296 S. parauberis isolates from Korean cultured olive ounder, 17 of which were found to have tet(M).…”

Section: Epidemiological Cut-off Values and Mar Indexmentioning

confidence: 83%

Distribution of antibiotic resistance gene against Streptococcus parauberis isolated from cultured olive flounder (Paralichthys olivaceus) in Jeju, South Korea

Kim

Jun

Lee

et al. 2023

Preprint

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Common bacterial diseases in the olive flounder Paralichthys olivaceus, a commercially important fish species, are caused by Streptococcus spp., Vibrio spp., and Edwardsiella piscicida, which cause significant economic losses in aquaculture. Antibiotics are mainly used to treat bacterial diseases. However, continued use of antibiotics causes bacterial resistance, which may lead to the transfer of antibiotic resistance genes. In our study, we isolated bacteria from the livers of cultured olive flounder from 34 farms on Jeju Island, South Korea. A total of 611 bacteria were isolated, of which 296 (48%) were Streptococcus parauberis by polymerase chain reaction (PCR) analysis. The isolates were tested for antibiotic susceptibility to 17 disks (including ampicillin, amoxicillin, erythromycin, tetracycline, oxytetracycline, and doxycycline) using disk diffusion. In addition, 13 antibiotic resistance genes were tested, including 9 tetracycline resistance genes (including tet(B) and tet(M)) and 4 macrolide resistance genes (including erm(B)). Data analysis of resistance levels were analyzed using normalized resistance interpretation (NRI) and multiple antibiotic resistance (MAR) index based on inhibition zones derived from the disk diffusion method. Antibiotic resistance genes were detected in all 296 isolates of S. parauberis. Tetracycline resistance genes were detected in 116 strains (39%) and macrolide resistance genes were identified in 96 strains (32%). The analysis of NRI resulted in COwt values for 13 antibiotics, excluding the COwt values for the four antibiotics sulfadiazine, oxolinic acid, nalidixic acid, and flumequine, which did not exhibit a normal distribution due to the dominance of non-wild types. Analysis of the multiple antibiotic resistance (MAR) showed that 39.9% of S. parauberis isolates had an MAR index of 0.22. The cut-off values presented in this study can be used as reference values for the disk diffusion method results because the experiment was performed on many S. parauberis. Future studies on antibiotic resistance of various fish pathogens should be conducted, with emphasis on alternate treatments.

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Structural characterization of Tn916-like element inStreptococcus parauberisserotype II strains isolated from diseased Japanese flounder

Cited by 5 publications

References 21 publications

Prevalence, antibiotic susceptibility and serotyping of Streptococcus parauberis isolates from diseased marine fish

Prevalence, antibiotic susceptibility and serotyping of Streptococcus parauberis isolates from diseased marine fish

Antimicrobial Resistance inStreptococcusspp

Distribution of antibiotic resistance gene against Streptococcus parauberis isolated from cultured olive flounder (Paralichthys olivaceus) in Jeju, South Korea

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