Structural compositions and biological activities of cell wall polysaccharides in the rhizome, stem, and leaf of Polygonatum odoratum (Mill.) Druce

Li, Jing; Hsiung, Shih-Yi; Kao, Mu-Rong; Xing, Xiaohui; Chang, Shu-Chieh; Wang, Damao; Hsieh, Pei-Yun; Liang, Pi‐Hui; Zhu, Zhiwei; Cheng, Ting-Jen Rachel; Shie, Jiun‐Jie; Liou, Jing‐Ping; Abbott, D. Wade; Kwon, Sung Won; Hsieh, Yves S. Y.

doi:10.1016/j.carres.2022.108662

Cited by 9 publications

(3 citation statements)

References 17 publications

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“…Without C-1 deuterium labeling, the EI-MS spectra of PMAAs in each pair became indistinguishable, necessitating conventional TFA hydrolysis-NaBD4 reduction alongside reductive hydrolysis, where deuterium labeling is not applicable, on the whole red seaweed cell wall. It is worth mentioning that there has been a preference for GC-MS-based quantitation in the analysis of PMAAs from unfractionated higher plant whole cell walls [24,26,27]. This method often uses relative peak area or, more commonly, relative molar percentages, assuming a direct proportionality between each PMAA's quantity and ratio of its TIC peak area to its molecular mass, according to a widely accepted protocol [13].…”

Section: Identification and Quantitation Of Pmaasmentioning

confidence: 99%

“…Conducting linkage analysis on unfractionated cell walls is crucial for understanding the relative compositions of all polysaccharides within the samples, as this approach eliminates the need for fractionation and the potential loss of specific polysaccharide fractions [20]. The procedure has been frequently used for analysis of unfractionated polysaccharides in the form of alcohol-insoluble residues (AIRs) prepared from higher plants and fungi, whose cell walls mainly consist of normal neutral monosaccharide, uronic acid, and amino sugar linkages [21][22][23][24][25][26][27]. However, the procedure cannot be directly applied to the linkage analysis of unfractionated polysaccharides of red seaweeds due to the presence of sulfated and/or anhydro sugar linkages in galactans.…”

Section: Introductionmentioning

confidence: 99%

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Methylation-GC-MS/FID-Based Glycosidic Linkage Analysis of Unfractionated Polysaccharides in Red Seaweeds

Bajwa,

Xing,

Terry

et al. 2024

Preprint

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Glycosidic linkage analysis was conducted on the unfractionated polysaccharides in the alcohol-insoluble residues (AIRs) prepared from six red seaweeds (Gracilariopsis sp., Prionitis sp., Mastocarpus papillatus, Callophyllis sp., Mazzaella splendens, and Palmaria palmata) using GC-MS/FID analysis of partially methylated alditol acetates (PMAAs). The cell walls of P. palmata primarily contained mixed-linkage xylans and small amounts of sulfated galactans and cellulose. In contrast, the unfractionated polysaccharides of the other five species were rich in galactans displaying diverse 3,6-anhydro-galactose and galactose linkages with varied sulfation patterns. Different levels of cellulose were also observed. This glycosidic linkage method offers advantages for cellulose analysis over traditional monosaccharide analysis that is known for underrepresenting glucose in crystalline cellulose. Relative linkage compositions calculated from GC-MS and GC-FID measurements showed that anhydro sugar linkages generated more response in the latter detection method. This improved linkage workflow presents a useful tool for studying polysaccharide structural variations across red seaweed species. Furthermore, for the first time, relative linkage compositions from GC-MS and GC-FID measurements, along with normalized FID and total ion current (TIC) chromatograms without peak assignments, were analyzed using principal component analysis (PCA) as a proof-of-concept demonstration of the technique's potential to differentiate various red seaweed species.

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Section: Identification and Quantitation Of Pmaasmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Methylation-GC-MS/FID-Based Glycosidic Linkage Analysis of Unfractionated Polysaccharides in Red Seaweeds

Bajwa,

Xing,

Terry

et al. 2024

Preprint

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“…Previously, scientists have shown the polysaccharide potentials in the stimulation of phagocytic activity (macrophage activation) by increased formation of ROS (reactive oxygen species), facilitating the release of cytokines and chemokines ( Devi et al, 2015 ). Furthermore, in vivo, studies have revealed the significant potential of natural polysaccharides in boosting the immune system in different animal trials with reduced immunity mitigated by viral disease, environmental pollution, or synthetic chemicals ( Li et al, 2022 ). Polysaccharides have been also linked with the reduction of free radicals and oxidative stress, an important risk factor for a gastrointestinal disorder (gastric ulcer) ( Ma et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Gastroprotective effects of Polygonatum odoratum in rodents by regulation of apoptotic proteins and inflammatory cytokines

Mariod

Jabbar

Alamri

et al. 2023

Saudi Journal of Biological Sciences

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Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2021–2022

Harvey

2024

Mass Spectrometry Reviews

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The use of matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry for the analysis of carbohydrates and glycoconjugates is a well‐established technique and this review is the 12th update of the original article published in 1999 and brings coverage of the literature to the end of 2022. As with previous review, this review also includes a few papers that describe methods appropriate to analysis by MALDI, such as sample preparation, even though the ionization method is not MALDI. The review follows the same format as previous reviews. It is divided into three sections: (1) general aspects such as theory of the MALDI process, matrices, derivatization, MALDI imaging, fragmentation, quantification and the use of computer software for structural identification. (2) Applications to various structural types such as oligo‐ and polysaccharides, glycoproteins, glycolipids, glycosides and biopharmaceuticals, and (3) other general areas such as medicine, industrial processes, natural products and glycan synthesis where MALDI is extensively used. Much of the material relating to applications is presented in tabular form. MALDI is still an ideal technique for carbohydrate analysis, particularly in its ability to produce single ions from each analyte and advancements in the technique and range of applications show little sign of diminishing.

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Structural compositions and biological activities of cell wall polysaccharides in the rhizome, stem, and leaf of Polygonatum odoratum (Mill.) Druce

Cited by 9 publications

References 17 publications

Methylation-GC-MS/FID-Based Glycosidic Linkage Analysis of Unfractionated Polysaccharides in Red Seaweeds

Methylation-GC-MS/FID-Based Glycosidic Linkage Analysis of Unfractionated Polysaccharides in Red Seaweeds

Gastroprotective effects of Polygonatum odoratum in rodents by regulation of apoptotic proteins and inflammatory cytokines

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2021–2022

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