A damaged member in a truss structure leads to a variation in the initial responses of its adjacent members. A flexibilitybased approach extracting from the modal data should be implemented as one of the structural damage detection methods. The frequency response function data as dynamic measurements provide more information on the system characteristics compared with modal data. Proper orthogonal modes from the frequency response functions extracted in the given frequency ranges and their modified forms can be utilized as damage indices to detect damages. This study considers damage detection of a truss structure using a frequency response function-based approach transformed to the proper orthogonal modes and a flexibility-based approach using the first few modal data for undamaged and damaged states. The utilization of these two methods is compared through numerical experiments on truss structures. The methods can rarely detect the damaged member accurately, but a group of damage-expected members is detected despite the existence of external noise. It is shown that the frequency response function-based approach can be utilized more explicitly than the flexibility-based approach.