Structural degradation and uptake of resveratrol-encapsulated liposomes using an in vitro digestion combined with Caco-2 cell absorption model

Effect of chewing ability on in vivo oral digestive characteristics and in vitro gastrointestinal starch hydrolysis of three different types of cooked rice

“…Simulated gastric fluid (SGF, pH of 1.5) and simulated intestinal fluid (SIF, pH of 7) were prepared according to the protocol in INFOGEST 2.0. 22,23 The stock solution was diluted to 400 mL with water, a 1.25× concentrate. Enzymes, bile salts, Ca 2+ solution and water were added to the stock solution and diluted to 1× before the experiment.…”

Recent advances in oral delivery systems of resveratrol: foreseeing their use in functional foods

“…128 Few studies have evaluated the behavior of formulations during the complete physiological process. Table 1 demonstrates that very few authors have reported in vitro digestion, followed by cellular interactions in the same study, where the studies reported by Huang et al 75 and Xu et al 129 are the only examples included in Table 1.…”

“…Food & Function lar antioxidant activity in human colon carcinoma cells (Caco-2 cells); however, the nanoparticles were tested in cells without previous digestion. Xu et al 129 tested different concentrations of digested resveratrol-loaded liposomes (5 to 90 µM) in Caco-2 cells, demonstrating the dose-dependent effect of resveratrol on cell viability (CCK-8 assay) (all concentrations were biocompatible) and assessed the antioxidant effect of free resveratrol and resveratrol loaded in liposomes (CellROX Deep Red assay), with improved results for the delivery systems of resveratrol versus free resveratrol.…”

Development details of an Artificial Gastric Digestive System (AGDS) and analysis of model food disintegration and degradation

“…These models have the advantages of saving time and cost and are currently used to study the digestion of food, nutrients, medicine and others (Guerra et al, 2012;Mackie et al, 2020). For example, DGM was built on the data collected from human digestion studies, and it has been used to assess the impact of bioaccessibility and delivery behaviour of meals, ranging from water to a high-fat breakfast, to the duodenum (Lo Curto et al, 2011;Xu et al, 2023). HGS has a latex-lined chamber to mimic the stomach, and 12 rollers secured on the belts provide extrusion force to simulate gastric peristalsis.…”

“…Therefore, in vitro studies are beneficial and facilitate data screening. Therefore, in vitro models have been developed as an alternative to in vivo studies (Liu et al ., 2020a, 2020b; Xu et al ., 2023). People initially used rigid containers such as simple stirring tanks and shaking water bath to simulate human stomach digestion (Chen et al ., 2016).…”