Structural DNA Nanotechnology: From Design to Applications

Zadegan, Reza M.; Norton, Michael L.

doi:10.3390/ijms13067149

Cited by 77 publications

(58 citation statements)

References 88 publications

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“…Enhancement of quantum dots in the presence of metal particles has been shown previously [1,8,17,18]. In these cases, the quantum dots were mixed in a PMMA layer deposited over the metal nanoparticles.…”

Section: Introductionmentioning

confidence: 86%

Fluorescence of quantum dots on e-beam patterned and DNA origami substrates

Corrigan

Kessinger

Kidd

et al. 2015

Image Sensing Technologies: Materials, Devices, Systems, and Applications II

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Attachment of quantum dots or fluorescent molecules to gold nanoparticles has a variety of optical labeling and sensory applications. In this study, we use both e-beam lithography and DNA origami to examine the fluorescence enhancement of fluorescent molecules and quantum dots with a systematic approach to understanding the contribution of gold nanoparticle size and interparticle spacing. The unique design of our patterns allows us to study the effects of size and spacing of the gold nanoparticles on the enhancement of fluorescence in one quick study with constant conditionsremoving undesirable effects such as differences in concentration of quantum dots or other chemistry differences that plague multiple experiments. We also discuss the fluorescence and bonding of CdSe/ZnS quantum dots to both gold as well as DNA for use in self assembled DNA constructs. Specifically, bioconjugated CdSe/ZnS core/shell quantum dots were synthesized and functionalized with MPA using both traditional ligand exchange as well as newly developed in situ functionalization techniques used to increase the quantum yield of the quantum dots. We will present fluorescent images showing results of optimal size and spacing for fluorescence as well as demonstrating attachment chemistry of the quantum dots. .

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Section: Introductionmentioning

confidence: 86%

Fluorescence of quantum dots on e-beam patterned and DNA origami substrates

Corrigan

Kessinger

Kidd

et al. 2015

Image Sensing Technologies: Materials, Devices, Systems, and Applications II

Self Cite

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“…While naturally occurring RNA motifs are useful for noncovalent and covalent connectors in assembly of RNA helices, 1D, 2D, and 3D nucleic acid nanostructures with geometric shapes have been extensively investigated using DNA . Thus, application of the design principles developed for geometric DNA nanostructures to RNA nanoobjects provides a design strategy without RNA motifs.…”

Section: Geometrical Rna Nanostructures Without Naturally Occurring Rmentioning

confidence: 99%

“…From the material point of view, RNA is chemically less stable than DNA, whose 2D and 3D nanostructures can be designed and constructed highly flexibly by using DNA‐origami or other methods . Construction of DNA nanostructures inside living cells, however, is still a highly challenging issue owing to a lack of cellular machinery to produce single‐stranded DNAs.…”

Section: Introductionmentioning

confidence: 99%

RNA Tectonics (tectoRNA) for RNA nanostructure design and its application in synthetic biology

2013

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RNA molecules are versatile biomaterials that act not only as DNA-like genetic materials but also have diverse functions in regulation of cellular biosystems. RNA is capable of regulating gene expression by sequence-specific hybridization. This feature allows the design of RNA-based artificial gene regulators (riboregulators). RNA can also build complex two-dimensional (2D) and 3D nanostructures, which afford protein-like functions and make RNA an attractive material for nanobiotechnology. RNA tectonics is a methodology in RNA nanobiotechnology for the design and construction of RNA nanostructures/nanoobjects through controlled self-assembly of modular RNA units (tectoRNAs). RNA nanostructures designed according to the concept of RNA tectonics are also attractive as tools in synthetic biology, but in vivo RNA tectonics is still in the early stages. This review presents a summary of the achievements of RNA tectonics and its related researches in vitro, and also introduces recent developments that facilitated the use of RNA nanostructures in bacterial cells.

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Section: Introductionmentioning

confidence: 99%

DNA-Scaffolded Proximity Assembly and Confinement of Multienzyme Reactions

Wang

Liang

et al. 2020

Top Curr Chem (Z)

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Cellular functions rely on a series of organized and regulated multienzyme cascade reactions. The catalytic efficiencies of these cascades depend on the precise spatial organization of the constituent enzymes, which is optimized to facilitate substrate transport and regulate activities. Mimicry of this organization in a non-living, artificial system would be very useful in a broad range of applications-with impacts on both the scientific community and society at large. Self-assembled DNA nanostructures are promising applications to organize biomolecular components into prescribed, multidimensional patterns. In this review, we focus on recent progress in the field of DNA-scaffolded assembly and confinement of multienzyme reactions. DNA self-assembly is exploited to build spatially organized multienzyme cascades with control over their relative distance, substrate diffusion paths, compartmentalization and activity actuation. The combination of addressable DNA assembly and multienzyme cascades can deliver breakthroughs toward the engineering of novel synthetic and biomimetic reactors.

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Structural DNA Nanotechnology: From Design to Applications

Cited by 77 publications

References 88 publications

Fluorescence of quantum dots on e-beam patterned and DNA origami substrates

Fluorescence of quantum dots on e-beam patterned and DNA origami substrates

RNA Tectonics (tectoRNA) for RNA nanostructure design and its application in synthetic biology

DNA-Scaffolded Proximity Assembly and Confinement of Multienzyme Reactions

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