2002
DOI: 10.1006/bbrc.2002.6472
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Structural Domains of Vault Proteins: A Role for the Coiled Coil Domain in Vault Assembly

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Cited by 52 publications
(84 citation statements)
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“…For this, we first searched any putative structural or functional domains in MVP. As reported by van Zon et al (42), the MVP molecule contains three putative EF hand repeats in the N-terminal half and a coiled-coil conformation in the C-terminal half. EF hand is a helix-loop-helix motif.…”
Section: The C2 Domain Of Pten Interacts With the Ef Hand Pair Of Mvp-supporting
confidence: 54%
See 1 more Smart Citation
“…For this, we first searched any putative structural or functional domains in MVP. As reported by van Zon et al (42), the MVP molecule contains three putative EF hand repeats in the N-terminal half and a coiled-coil conformation in the C-terminal half. EF hand is a helix-loop-helix motif.…”
Section: The C2 Domain Of Pten Interacts With the Ef Hand Pair Of Mvp-supporting
confidence: 54%
“…Each vault particle contains ϳeight VPARP, two telomerase-associated protein 1, and 96 MVP molecules. Recently, van Zon et al (42) characterized the function of each structural domain of these three proteins for the assembly of the vault particles. It was found that MVP self-associates through its C-terminal coiled-coil sequence and interacts with VPARP through its N-terminal region.…”
Section: Fig 4 Pten Interacts With Mvp In Vitro In a Camentioning
confidence: 99%
“…All attempts have thus far been unsuccessful (data not shown). As MVP has been shown to bind calcium, we have also treated vaults with various concentrations of EGTA or calcium chloride to no avail (36). We considered the possibility that since endogenous vaults in extracts are much less protective of the associated VR than are purified vaults, a soluble factor that facilitates the opening of vaults could be present in extracts and then lost during purification.…”
Section: Discussionmentioning
confidence: 99%
“…Primers are listed in Supplementary Table 1. Y2H analysis Y2H was performed using yeast strain PJ69-4A (Dr Philip James, University of Wisconsin, Madison, WI, USA) as described (James et al, 1996;van Zon et al, 2002). As prey library, a mouse 17-day embryo cDNA library (Matchmaker, Clontech Laboratories, Palo Alto, CA, USA) was used.…”
Section: Pcr Primersmentioning
confidence: 99%