Structural, enzymatic and biological properties of new PLA2 isoform from Crotalus durissus terrificus venom

Crotoxin from Crotalus durissus terrificus and Crotoxin-Related Proteins: Structure and Function Relationship

“…Differences in the stability and neurotoxicity of crotoxin complexes result from the point mutations in CB isoforms (see below). F15, F16, and F17 PLA 2 s in complex with CA reveal much lower toxicity (Oliveira et al 2002;Toyama et al 2003;Hernandez-Oliveira et al 2005), whereas Intercro does not display neurotoxic properties (Vieira et al 2013). Surprisingly, the toxicity of the Gintexin complex is similar to that observed for crotoxin (Yang et al 2015a).…”

“…The CB isoforms are products of different mRNAs (Bouchier et al 1991;. However, in Crotalus durissus terrificus venom, several additional CB isoforms (CB-like proteins: F15 (Toyama et al 2003), F16 (Hernandez-Oliveira et al 2005, F17 (Oliveira et al 2002), and Intercro (Vieira et al 2013)) have been identified. Proteins F15, F16, and F17 were isolated from the crotoxin fraction by RP-HPLC.…”

“…In addition to neurotoxicity, crotoxin exhibits other significant pharmacological properties and biological effects such as myotoxic (Gutiérrez et al 2008), cardiotoxic (potentiation of L-type Ca 2+ channel) (Zhang et al 2010), cytotoxic (chronic seizure effect by release of glutamate from cerebrocortical synaptosomes via N and P/Q Ca 2+ channel in the central nervous system) (Lomeo Rda et al 2014), bactericidal (Oliveira et al 2002;Toyama et al 2003;Perumal Samy et al 2006), anti-inflammatory (Cardoso et al 2001;Sampaio et al 2005;Zambelli et al 2008;Nunes et al 2010), antitumoral (Rudd et al 1994;Costa et al 1998;Cura et al 2002;Yan et al 2007), and analgesic (Zhang et al 2006;Nogueira-Neto Fde et al 2008) effects and antiviral activity (Muller et al 2012;Muller et al 2014).…”

Evaluation of anti-inflammatory activity of derivatives from aerial parts ofBaccharis uncinella

“…The activities of CH 2 Cl 2 -Bu and EtOAc-Bu phases against the induction of inflammation by highly purified PLA2 from rattlesnake Crotalus durissus terrificus (Toyama et al, 2003) were evaluated. Following the procedure described by Iglesias et al (2005), samples of PLA2 were incubated with fractions CH 2 Cl 2 -Bu, EtOAc-Bu for 30 min at 37°C and subsequently re-purified to yield samples referred to as PLA2-CH 2 Cl 2 (for PLA2 treated with CH 2 Cl 2 -Bu) and PLA2-EtOAc (for PLA2 treated with EtOAc-Bu).…”

“…Measurement of sPLA2 activity sPLA2 activity was measured following the protocols described by Toyama et al (2003) for 96-well plate, using as substrate 4-nitro-3-octanoyloxy-benzoic acid 604 C.A. Zalewski et al…”