2000
DOI: 10.1126/science.289.5484.1546
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Structural Evidence for Evolution of the β/α Barrel Scaffold by Gene Duplication and Fusion

Abstract: The atomic structures of two proteins in the histidine biosynthesis pathway consist of beta/alpha barrels with a twofold repeat pattern. It is likely that these proteins evolved by twofold gene duplication and gene fusion from a common half-barrel ancestor. These ancestral domains are not visible as independent domains in the extant proteins but can be inferred from a combination of sequence and structural analysis. The detection of subdomain structures may be useful in efforts to search genome sequences for f… Show more

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Cited by 305 publications
(379 citation statements)
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“…The results suggest that the members of several important superfamilies and probably a large fraction of all known (βα) 8 -barrels have a common evolutionary origin (Altamirano et al, 2000;Babbitt and Gerlt, 2000;Copley and Bork, 2000;Jürgens et al, 2000). Moreover, evidence for the evolution of the (βα) 8 -barrel fold from ancestral 'halfbarrel' precursors will be presented (Thoma et al, 1998;Lang et al, 2000;Höcker et al, 2001). …”
Section: Introductionmentioning
confidence: 99%
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“…The results suggest that the members of several important superfamilies and probably a large fraction of all known (βα) 8 -barrels have a common evolutionary origin (Altamirano et al, 2000;Babbitt and Gerlt, 2000;Copley and Bork, 2000;Jürgens et al, 2000). Moreover, evidence for the evolution of the (βα) 8 -barrel fold from ancestral 'halfbarrel' precursors will be presented (Thoma et al, 1998;Lang et al, 2000;Höcker et al, 2001). …”
Section: Introductionmentioning
confidence: 99%
“…Therefore, both enzymes were tested for their mutual residual activities. Whereas HisA does not show detectable HisF activity, HisF catalyses the HisA reaction, albeit with low efficiency (Lang et al, 2000).…”
Section: The Phosphate-binding Superfamiliymentioning
confidence: 99%
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“…Note that water molecules are present in the cavities (Table III) according to the crystal structure. 5 The walls of the three cavities are formed predominantly by hydrophobic residues but contain also a few hydrophilic amino acids. Xenon is, however, able to bind into amphiphilic regions despite its clear preference for hydrophobic environments.…”
Section: Identification Of Xenon Binding Sitesmentioning
confidence: 99%
“…2) with the C-terminal (catalytic) face forming the active site and the N-terminal (stability) face interacting with HisH. 5,6 The active sites of HisH and HisF are separated by a distance of $25 Å . 6,7 Solution NMR spectroscopy was used to assign the 1 H, 15 N, and 13 C resonances of HisF and to study the influence of ImGP binding and complex formation between the two subunits on the protein dynamics of HisF.…”
Section: Introductionmentioning
confidence: 99%