Structural Evolution of Yeast Alcohol Dehydrogenase in the Laboratory

Wills, Christopher

doi:10.1007/978-1-4684-4844-3_8

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Selection and Adaptation1

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1988

2000

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Cited by 2 publications

(1 citation statement)

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“…Within 400 generations, a mutant acid phosphatase with 60% greater activity at pH 6.0 and a pH optimum shifted to 4.8 was identified (a subsequent mutation caused clumping of the cells, reducing their likelihood of being washed out of the chemostat and illustrating that experimental evolution is somewhat like computer programming: you get exactly what you ask for, whether or not that was what you truly wanted). Selecting for mutant enzymes that discriminate between the natural substrate ethanol and the toxic analogue allyl alcohol63, 64 has altered the substrate specificity of alcohol dehydrogenase.…”

Section: Selection and Adaptationmentioning

confidence: 99%

Budding yeast as a model organism for population genetics

Zeyl

2000

Yeast

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Section: Selection and Adaptationmentioning

confidence: 99%

Budding yeast as a model organism for population genetics

Zeyl

2000

Yeast

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An efficient selection producing structural gene mutants of yeast alcohol dehydrogenase resistant to pyrazole.

Wills¹,

Hom²

1988

Genetics

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Selection for resistance to allyl alcohol in respiration-incompetent Saccharomyces cerevisiae produces a high proportion of mutants that can be localized within the ADH2 structural gene and that still, because of the type of selection employed, retain enzyme activity. We show here that a similar type of selection produces a similarly high proportion of mutants resistant to the competitive inhibitor pyrazole. The first four mutants examined, picked at random from a collection of spontaneous pyrazole-resistant mutants, show altered--usually increased--KM values for ethanol and NAD+, and markedly increased K1 values for pyrazole, compared with the wild type. When these kinetic measures and their electrophoretic mobilities were compared, all the mutants could be clearly distinguished from each other as well as from wild type. Genetic analysis shows these mutants to be close to and probably resident in the structural gene. For a variety of reasons, these mutants are even more favorable subjects for population genetic analysis and the dissection of molecular microevolution than are allyl alcohol-resistant mutants.

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Structural Evolution of Yeast Alcohol Dehydrogenase in the Laboratory

Cited by 2 publications

References 35 publications

Budding yeast as a model organism for population genetics

Budding yeast as a model organism for population genetics

An efficient selection producing structural gene mutants of yeast alcohol dehydrogenase resistant to pyrazole.

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