Structural features and immunological perception of the cell surface glycans of Lactobacillus plantarum: a novel rhamnose-rich polysaccharide and teichoic acids

Selection of Exopolysaccharide-Producing Lactobacillus Plantarum (Lactiplantibacillus Plantarum) Isolated from Algerian Fermented Foods for the Manufacture of Skim-Milk Fermented Products

“…Regarding the structure of the EPSs, their repeating unit likely presents a phosphodiester linkage, as suggested by the peculiar shape of the signal at 5.44 ppm that is representative of a sugar in the α-configuration attached to a phosphate group. This finding is not unusual since there are several cases in LAB strains in which these non-carbohydrate substituents are part of EPS/CPS structures [ 14 , 54 , 55 ] or where two repeating units are joined by a phosphodiester linkage [ 50 ].…”

“…The physical-chemical properties of EPSs purified from different L. plantarum strains have been characterized and all of them presented glucose and galactose in their composition [ 8 , 44 ], such as we have denoted in our polymers. Very often, these monosaccharides are combined with other monomers such as mannose [ 8 ], fructose [ 57 ], N-acetyl-galactosamine [ 58 ], galactosamine, glucosamine [ 47 ], rhamnose [ 50 ], glucuronic acid [ 59 ] and/or glycerol [ 50 , 58 ], among others. The physical-chemical characteristics of the EPS are of special relevance for their functional and technological characteristics [ 14 , 60 ].…”

“…Given that in this work an additional treatment with DNAse and pronase E, followed by 12%-TCA precipitation, was performed for EPS purification, this suggests that proteins could be strongly linked to the surface structures extracted. In this regard, some authors have reported that proteins could form part of cell-wall polysaccharides [48], although other surface structures (such as teichoic acids) could have co-precipitated with the polysaccharides [49,50]. Nevertheless, most authors do not report checking the presence of proteins in the EPS they have characterized.…”

Harnessing the Potential of Lactobacillus Species for Therapeutic Delivery at the lumenal-mucosal Interface

“…The Ginsburg (2002) review in particular outlines the role of LTA in inflammation and parallels its activity with that of endotoxin A from Gram-negative bacteria. Interestingly, recent studies have shown LTA from different L. plantarum strains to have anti-inflammatory effects in some experimental conditions [86,91,92] while noting upregulation of both pro-and anti-inflammatory cytokines in others [93,94]. Investigators have also noted LTA-induced mucin production in mouse models that ultimately decreased the breakdown of the intestinal barrier and downstream symptoms such as cognitive function [92,95].…”

Data_Sheet_1.PDF

“…L-rhamnose is a common 6-deoxy hexose found in the cell wall polysaccharides of many bacteria and the carbohydrate moieties of many natural products from bacteria and plants (Halimah et al, 2015;Khalil et al, 2015;Wittgens et al, 2018;Edgar et al, 2019;Garcia-Vello et al, 2020). These rhamnosecontaining biomolecules show great potential in the development of drugs and vaccines owing to their distinct bioactive properties (Wang et al, 2011;Lee et al, 2014;Mei et al, 2019;Pequegnat and Monteiro, 2019).…”