Structural features of Dnase1L3 responsible for diverse antigenic serum DNA clearance

McCord, Jon J.; Engavale, Minal; Masoumzadeh, Elahe; Villarreal, Johanna; Mapp, Britney; Latham, Michael P.; Keyel, Peter A.; Sutton, R. Bryan

doi:10.1016/j.bpj.2021.11.568

Cited by 2 publications

(1 citation statement)

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“…Together these results suggest eDNA is a component of the epididymal amyloid matrix and is present in several distinct populations including that which is resistant to, or protected from, DNase. The preferential ability of DNase1L3 to digest eDNA in the P4 amyloid over that of DNase1 is consistent with its known ability to degrade DNA complexed to protein (37).…”

Section: Resultssupporting

confidence: 70%

The Mouse Epididymal Amyloid Matrix: A Mammalian Counterpart of a Bacterial Biofilm

Myers,

Atkins,

Villarreal

et al. 2023

Preprint

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The mouse epididymis is a long tubule connecting the testis to the vas deferens. Its primary functions are to mature spermatozoa into motile and fertile cells and to protect them from pathogens that ascend the male tract. We previously demonstrated that a functional extracellular amyloid matrix surrounds spermatozoa in the epididymal lumen and has host defense functions; properties not unlike that of an extracellular biofilm that surrounds and protects a bacterial community. Here we show the epididymal amyloid matrix also structurally resembles a biofilm by containing eDNA, eRNA, and mucin-like polysaccharides. Further these structural components exhibit comparable behaviors and perform functions like their counterparts in bacterial biofilms. Our studies suggest that nature has used the ancient building blocks of bacterial biofilms to form an analogous structure that nurtures and protects the mammalian male germline.

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Section: Resultssupporting

confidence: 70%