2004
DOI: 10.1128/mcb.24.9.3907-3917.2004
|View full text |Cite
|
Sign up to set email alerts
|

Structural Features of Nucleosomes Reorganized by Yeast FACT and Its HMG Box Component, Nhp6

Abstract: The Saccharomyces cerevisiae Spt16/Cdc68, Pob3, and Nhp6 proteins (SPN or yFACT) bind to and alter nucleosomes in vitro, providing a potential explanation for their importance in both transcription and replication in vivo. We show that nucleosomes bound by either Nhp6 alone or the yFACT complex remain largely intact and immobile but are significantly reorganized, as indicated by changes in the pattern of sensitivity to DNase I and enhanced digestion by some restriction endonucleases. In contrast, yFACT enhance… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1

Citation Types

10
89
0
1

Year Published

2004
2004
2013
2013

Publication Types

Select...
4
3

Relationship

2
5

Authors

Journals

citations
Cited by 75 publications
(102 citation statements)
references
References 31 publications
10
89
0
1
Order By: Relevance
“…The spt16-11 (T828I, P859S) (Formosa et al 2002), hta1-V101I, and htb1-A84D mutations were introduced into expression constructs using the Quikchange strategy (Stratagene), and the proteins were purified as previously described (Ruone et al 2003;Rhoades et al 2004;Xin et al 2009). …”
Section: Methodsmentioning
confidence: 99%
See 3 more Smart Citations
“…The spt16-11 (T828I, P859S) (Formosa et al 2002), hta1-V101I, and htb1-A84D mutations were introduced into expression constructs using the Quikchange strategy (Stratagene), and the proteins were purified as previously described (Ruone et al 2003;Rhoades et al 2004;Xin et al 2009). …”
Section: Methodsmentioning
confidence: 99%
“…Nucleosomes were reconstituted in vitro with recombinant histones, labeled with fluorescent dyes, and tested for binding affinity with FACT, sensitivity to DraI digestion, and retention of H2A-H2B dimers as described previously (Ruone et al 2003;Rhoades et al 2004;Xin et al 2009). The spt16-11 (T828I, P859S) (Formosa et al 2002), hta1-V101I, and htb1-A84D mutations were introduced into expression constructs using the Quikchange strategy (Stratagene), and the proteins were purified as previously described (Ruone et al 2003;Rhoades et al 2004;Xin et al 2009).…”
Section: Methodsmentioning
confidence: 99%
See 2 more Smart Citations
“…The yeast FACT (yFACT) complex consists of two subunits, Spt16 and Pob3, and an associated high-mobility group (HMG) protein Nhp6 (Brewster et al 2001;Formosa et al 2001). In vitro studies show that yFACT alters the accessibility of DNA within nucleosomes without hydrolyzing ATP and without repositioning the histone octamer core relative to the DNA (Formosa et al 2001;Rhoades et al 2004;Biswas et al 2005).…”
mentioning
confidence: 99%