Structural framework for the understanding spectroscopic and functional signatures of the cyanobacterial Orange Carotenoid Protein families

Sluchanko, Nikolai N.; Maksimov, Eugene G.; Slonimskiy, Yury B.; Varfolomeeva, Larisa A.; Bukhanko, Antonina Y.; Egorkin, Nikita A.; Tsoraev, Georgy V.; Khrenova, Maria G.; Ge, Baosheng; Qin, Song; Boyko, Konstantin M.; Popov, Vladimir O.

doi:10.1016/j.ijbiomac.2023.127874

Cited by 5 publications

(2 citation statements)

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“…A similar thermal stability (T 0.5 = 66-67 °C) was found for the recently described Orange Carotenoid Protein 2 (OCP2) complexed with ECH, from Gloeocapsa sp. PCC 7418 inhabiting moderately hot springs 49 (Supplementary Fig. 4).…”

Section: Resultsmentioning

confidence: 99%

“…The ZEA-bound form of the Carotenoid-Binding Protein from Bombyx mori (termed BmCBP; residues 68-297 of Uniprot Q8MYA9) and the ECH-bound form of the Orange Carotenoid Protein 2 from Gloeocapsa sp. PCC 7428 (OCP2; residues 1-318 of Uniprot K9XH36) were obtained as electrophoretically homogeneous preparations in previous works 40,49 . In the final form, all proteins had His-tags cleaved off.…”

Section: Cloning Protein Expression and Purificationmentioning

confidence: 99%

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Insights into the molecular mechanism of yellow cuticle coloration by a chitin-binding carotenoprotein in gregarious locusts

Egorkin,

Dominnik,

Maksimov

et al. 2024

Commun Biol

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Carotenoids are hydrophobic pigments binding to diverse carotenoproteins, many of which remain unexplored. Focusing on yellow gregarious locusts accumulating cuticular carotenoids, here we use engineered Escherichia coli cells to reconstitute a functional water-soluble β-carotene-binding protein, BBP. HPLC and Raman spectroscopy confirmed that recombinant BBP avidly binds β-carotene, inducing the unusual vibronic structure of its absorbance spectrum, just like native BBP extracted from the locust cuticles. Bound to recombinant BBP, β-carotene exhibits pronounced circular dichroism and allows BBP to withstand heating (T0.5 = 68 °C), detergents and pH variations. Using bacteria producing distinct xanthophylls we demonstrate that, while β-carotene is the preferred carotenoid, BBP can also extract from membranes ketocarotenoids and, very poorly, hydroxycarotenoids. We show that BBP-carotenoid complex reversibly binds to chitin, but not to chitosan, implying the role for chitin acetyl groups in cuticular BBP deposition. Reconstructing such locust coloration mechanism in vitro paves the way for structural studies and BBP applications.

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Section: Resultsmentioning

confidence: 99%

Section: Cloning Protein Expression and Purificationmentioning

confidence: 99%