Structural-genetic insight and optimization of protease production from a novel strain of Aeromonas veronii CMF, a gut isolate of Chrysomya megacephala

Bhattacherjee, Rashmi; Mandal, Sanjay K.; Banerjee, Sandipan; Saha, Kunal Kumar; Sarkar, Jagannath; Banerjee, Dhriti; Mandal, Narayan Chandra

doi:10.1007/s00203-021-02282-x

Cited by 9 publications

(3 citation statements)

References 66 publications

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“…The morphological analysis illustrates the structure of this A. veronii rod-shaped, and the average length and width varied from 1.0-3.5 µm and 0.3-3.5 µm, respectively [53,54]. A similar observation in this study was noticed by SEM analysis of the isolated bacterial strain (MP3) from L. rohita with the character of producing biofilm.…”

Section: Discussionsupporting

confidence: 87%

Aeromonas veronii Is a Lethal Pathogen Isolated from Gut of Infected Labeo rohita: Molecular Insight to Understand the Bacterial Virulence and Its Induced Host Immunity

et al. 2023

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A case of severe mortality in farmed Labeo rohita was investigated to characterize the causative agent. We identified the bacterial strain as Aeromonas veronii isolated from the gut of infected L. rohita by biochemical assay, scanning electron microscopy and 16S rRNA gene sequence analysis. The in vivo challenge experiment showed that the LD50 of A. veronii was 2.2 × 104 CFU/fish. Virulence gene investigation revealed that the isolated A. veronii possesses Aerolysin, Cytotoxic enterotoxin, Serine protease, Dnase and Type III secretion system genes. The isolated strain was resistant to two antibiotics (ampicillin and dicloxacillin) while susceptible to 22 other antibiotics. The study further revealed that A. veronii induced both stresses along with non-specific and specific immune responses marked by elevated cortisol HSP70, HSP90 and IgM levels in the treated L. rohita fingerlings. Although the bacterial pathogen enhances the immune response, the negative effect on fish, including stress, and high mortality, create concern and a need for A. veronii management in L. rohita farms. The knowledge gained from this study would facilitate future research aimed at assessing the pathogenicity of A. veronii, with an emphasis on microbial disease management in other farmed fish species.

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Section: Discussionsupporting

confidence: 87%

Aeromonas veronii Is a Lethal Pathogen Isolated from Gut of Infected Labeo rohita: Molecular Insight to Understand the Bacterial Virulence and Its Induced Host Immunity

et al. 2023

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“…Proteolytic activation of toxins. Promotes evasion of initial host defenses by inactivating the complement system Bhattacherjee et al ( 2021 ); Sakai ( 1985 ); Shao et al ( 2023 ) ela Elastase Allows for evasion of immune defenses by cleaving IgA. Stimulates alginate synthesis Barger et al ( 2021 ) lip Lipase Generation of free fatty acid through lipolytic activity.…”

Section: Quorum Sensingmentioning

confidence: 99%

Antimicrobial resistance in aeromonads and new therapies targeting quorum sensing

Neil,

Cheney,

Rosenzweig

et al. 2024

Appl Microbiol Biotechnol

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Aeromonas species (spp.) are well-known fish pathogens, several of which have been recognized as emerging human pathogens. The organism is capable of causing a wide spectrum of diseases in humans, ranging from gastroenteritis, wound infections, and septicemia to devastating necrotizing fasciitis. The systemic form of infection is often fatal, particularly in patients with underlying chronic diseases. Indeed, recent trends demonstrate rising numbers of hospital-acquired Aeromonas infections, especially in immuno-compromised individuals. Additionally, Aeromonas-associated antibiotic resistance is an increasing challenge in combating both fish and human infections. The acquisition of antibiotic resistance is related to Aeromonas’ innate transformative properties including its ability to share plasmids and integron-related gene cassettes between species and with the environment. As a result, alternatives to antibiotic treatments are desperately needed. In that vein, many treatments have been proposed and studied extensively in the fish-farming industry, including treatments that target Aeromonas quorum sensing. In this review, we discuss current strategies targeting quorum sensing inhibition and propose that such studies empower the development of novel chemotherapeutic approaches to combat drug-resistant Aeromonas spp. infections in humans. Key points • Aeromonas notoriously acquires and maintains antimicrobial resistance, making treatment options limited. • Quorum sensing is an essential virulence mechanism in Aeromonas infections. • Inhibiting quorum sensing can be an effective strategy in combating Aeromonas infections in animals and humans.

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“…Adapun pada bagian saluran pencernaannya ditemukan Aeromonas veronii CMF dengan kemampuan proteolitik (Bhattacherjee et al, 2021). Jumlah dan jenis bakteri yang berasosiasi dengan lalat dapat dipengaruhi oleh perbedaan lokasi dan habitat lalat serta posisi keberadaan bakteri tersebut pada bagian tubuh internal atau eksternal (Monyama et al, 2021;Park et al, 2012).…”

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Kajian bakteri proteolitik yang diisolasi dari tubuh lalat hijau (Chrysomya megacephala)

Hidayati

Nurhimaya²,

Mahdi³

et al. 2022

JBIOUNUD

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Lalat hijau (Chrysomya megacephala) merupakan serangga yang memiliki peranan ekologis penting, salah satu diantaranya adalah sebagai dekomposer. Lalat hijau sering ditemukan berkerumun di sekitar makanan dan sampah yang mengandung protein tinggi. Hinggapnya lalat ini pada makanan perlu diwaspadai karena dapat mengakibatkan makanan lebih cepat rusak atau basi. Diduga bahwa bakteri yang ada pada tubuh lalat berperan dalam proses dekomposisi bahan makanan tersebut. Penelitian ini bertujuan untuk mempelajari bakteri proteolitik yang diisolasi dari tubuh lalat hijau. Sampel lalat hijau diambil dari tempat pembuangan sampah di Kebon Kongok, Lombok Barat, Indonesia. Sampel dimasukkan ke dalam Brain Heart Infusion Broth, selanjutnya bakteri yang terdapat pada tubuh lalat hijau diisolasi menggunakan Nutrient Agar. Aktivitas proteolitik isolat bakteri dideteksi dari terbentuknya zona bening pada medium Skim Milk Agar dengan metode totol dan difusi sumuran. Isolat bakteri kemudian dikarakterisasi melalui pengecatan Gram dan uji biokimia. Ditemukan sebanyak empat isolat bakteri proteolitik pada tubuh lalat hijau yaitu isolat LH1, LH2, LH3 dan LH4. Isolat LH2 menunjukkan aktivitas katalitik paling baik dengan rerata diameter zona bening sebesar 25,5 mm setelah inkubasi 48 jam pada suhu 37oC. LH2 merupakan Gram negatif berbentuk batang rantai pendek, bersifat motil dan aerob. Bakteri ini mampu memanfaatkan beberapa jenis gula seperti arabinosa, sukrosa, maltosa, dan manitol, mampu mengoksidasi asam amino triptofan serta mampu mengubah urea menjadi amonia. Hasil penelitian ini memberikan informasi bahwa, LH2 berkontribusi dalam proses dekomposisi. Selain itu, LH2 juga berpotensi sebagai patogen.

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Structural-genetic insight and optimization of protease production from a novel strain of Aeromonas veronii CMF, a gut isolate of Chrysomya megacephala

Cited by 9 publications

References 66 publications

Aeromonas veronii Is a Lethal Pathogen Isolated from Gut of Infected Labeo rohita: Molecular Insight to Understand the Bacterial Virulence and Its Induced Host Immunity

Aeromonas veronii Is a Lethal Pathogen Isolated from Gut of Infected Labeo rohita: Molecular Insight to Understand the Bacterial Virulence and Its Induced Host Immunity

Antimicrobial resistance in aeromonads and new therapies targeting quorum sensing

Kajian bakteri proteolitik yang diisolasi dari tubuh lalat hijau (Chrysomya megacephala)

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