The effect of microstructure on the impact toughness and the temper embrittlement of a SA508Gr.4N steel was investigated. Martensitic and bainitic structures formed in this material were examined via scanning electron microscopy, electron backscatter diffraction, transmission electron microscopy, and Auger electron spectroscopy (AES) analysis. The martensitic structure had a positive effect on both the strength and toughness. Compared with the bainitic structure, this structure consisted of smaller blocks and more high-angle grain boundaries (HAGBs). Changes in the ultimate tensile strength and toughness of the martensitic structure were attributed to an increase in the crack propagation path. This increase resulted from an increased number of HAGBs and refinement of the sub-structure (block). The AES results revealed that sulfur segregation is higher in the martensitic structure than in the bainitic structure. Therefore, the martensitic structure is more susceptible to temper embrittlement than the bainitic structure.Reactor pressure vessels (RPV) are generally considered to be the most critical components in the nuclear plant beside the reactor core 1,2 . The RPV should serve for 40 years under high-temperature, high-pressure, and neutron-irradiation conditions. Therefore, to ensure safe operation of the reactor, the RPV should be fabricated from material with high strength, high fracture toughness, and high resistance to irradiation embrittlement 3-5 .The RPV is typically fabricated from ASME SA508 Class 3 steel [6][7][8] . With the development of generation IV reactors, larger and higher-power nuclear power plants (compared with those housing generation III reactors) are being built. However, the low strength (620 MPa) of the SA508 Class 3 steel renders this material unsuitable for generation IV reactors and, hence, new materials must be developed. Many countries have considered SA508Gr.4N steel as a candidate material 9,10 .The RPVs are large forgings and with wall thickness of more than 200 mm 11 . Generally, the forged RPVs are quenched and tempered, as result, the through-thickness microstructure vary significantly because of the differential cooling rates during quenching. These differing microstructures will lead to heterogeneous mechanical properties along the thickness direction of the RPV. Therefore, an understanding of the microstructure and mechanical properties of RPV steels with different quenched microstructures is essential.The microstructure of the initial quenched martensite is a key factor in determining the performance of RPVs. Park et al. 12 reported that the strength of a quenched Ni-Cr-Mo low alloy steel with a fully martensitic microstructure is significantly higher than that of the quenched microstructure composed of bainite. Similarly, compared with bainite, tempered martensite is more susceptible to temper embrittlement. Raoul et al. 13 found that, in high-P (0.017%) A533 steel, P segregation in martensite is considerably higher than that in bainite. Furthermore, Lee et al. 14 dete...