Structural investigation on the carbohydrate backbone of the lipopolysaccharide from Klebsiella pneumoniae rough mutant R20/O1−

Susskind, Miriam M.; Müller‐Loennies, Sven; Nimmich, Wolfgang; Brade, Helmut; Holst, Otto

doi:10.1016/0008-6215(95)00002-b

Cited by 36 publications

(35 citation statements)

References 25 publications

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“…The only structural data for the Klebsiella core oligosaccharide are a partial structure from an O1 isolate (44) and a complete structure from a related O8 isolate (40). The larger O8 structure contains the region first identified in O1.…”

Section: Discussionmentioning

confidence: 99%

O antigen seroepidemiology of Klebsiella clinical isolates and implications for immunoprophylaxis of Klebsiella infections

Trautmann

Held

Cross

2004

Vaccine

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Section: Discussionmentioning

confidence: 99%

O antigen seroepidemiology of Klebsiella clinical isolates and implications for immunoprophylaxis of Klebsiella infections

Trautmann

Held

Cross

2004

Vaccine

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“…pneumoniae rough strain R20 (O1 Ϫ :K20 Ϫ ) was obtained from W. Nimmich (Institute of Medical Microbiology, University of Rostock, Germany). Its cultivation and the isolation of LPS from dried bacteria have been reported earlier (4). LPS from E. coli with core types R1, R2, R3, R4, and K-12 strain W3100, and S. enterica sv.…”

Section: Methodsmentioning

confidence: 98%

“…Capsular polysaccharides and LPS 1 are important virulence factors of K. pneumoniae (1,2), and structures of the former (3) and of O-antigens (see Ref. 4 for references) from LPS have been investigated extensively. A structural investigation of the LPS core region began only recently (4,5).…”

mentioning

confidence: 99%

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Identification of a Novel Heptoglycan of α1→2-Linkedd-glycero-d-manno-Heptopyranose

Susskind

Brade

et al. 1998

Journal of Biological Chemistry

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After immunization of BALB/c mice, two monoclonal antibodies were obtained that were shown to be specific for the core of LPS from K. pneumoniae ssp. pneumoniae, since they did not react with LPS or whole-cell lysates of a variety of other Gram-negative species. Both monoclonal antibodies could be inhibited by LPS but not by isolated oligosaccharides and are thus considered to recognize a conformational epitope in the core region.

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“…6 The molecular structure of the enterobacterial innercore region usually contains a trisaccharide composed of three α-Kdop linked units, as in the structure of the LPS of Klebsiella pneumoniae ssp Pneumoniae Strain R.20. 7 The LPS of Chlamydia trachomatis was shown to contain a disaccharide composed of two α-Kdop linked units, which were identified by gas chromatography mass spectrometry (GC-MS). 8 A feature of some inner core regions of enterobacterial lipopolysaccharides such as the core oligosaccharide of Yersinia ruckerii, Serotype II, is the presence of at least one residue of Kdo.…”

Section: Introductionmentioning

confidence: 99%

Characterization of the O-4 Phosphorylated and O-5 Substituted Kdo Reducing End Group and Sequencing of the Core Oligosaccharide of Aeromonas Salmonicida ssp Salmonicida Lipopolysaccharide Using Tandem Mass Spectrometry

Banoub

Aneed

Cohen

et al. 2004

Eur J Mass Spectrom (Chichester)

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The molecular structure of the wild strain of the lipopolysaccharide (LPS) core of Aeromonas salmonicida, ssp salmonicida has been sequenced using tandem mass spectrometry. The core oligosaccharide was determined to contain an O-4 phosphorylated and O-5 substituted Kdo reducing group and its structure is proposed as follows:After the core oligosaccharide of LPS was released from the lipid A portion by conventional treatment with 1% acetic acid, we demonstrated the existence of a homogeneous mixture composed mainly of the native core oligosaccharide containing the Kdo with its O-4 phosphate group intact and a degraded core oligosaccharide mixture which eliminated the O-4 phosphate group with extreme ease. The precise molecular structure and glycone sequence of the homogeneous mixture of phosphorylated and dephosphorylated core oligosaccharides was determined by electrospray ionization mass spectrometry and tandem mass spectrometric (MS/MS) analysis. Collision-induced dissociation MS/MS of the homogeneous mixture of permethylated core oligosaccharides afforded a series of diagnostic product ions which confirmed the established sequence of the glycones to be determined. Matrix-assisted laser desorption/ionization MS/MS reconfirmed the molecular structure of the dephosphorylated homogeneous permethylated mixture of the core oligosaccharides containing the diastereomeric 4,8-and 4,7-anhydro-α-keto acids.Keywords: structural investigation, Aeromonas salmonicida ssp salmonicida, lipopolysaccharide, native phosphorylated core oligosaccharide, degraded dephosphorylated core oligosaccharide, electrospray and matrix-assisted laser desorption/ionization, mass spectrometry and tandem mass spectrometry L-α-D-Hepp

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Structural investigation on the carbohydrate backbone of the lipopolysaccharide from Klebsiella pneumoniae rough mutant R20/O1−

Cited by 36 publications

References 25 publications

O antigen seroepidemiology of Klebsiella clinical isolates and implications for immunoprophylaxis of Klebsiella infections

O antigen seroepidemiology of Klebsiella clinical isolates and implications for immunoprophylaxis of Klebsiella infections

Identification of a Novel Heptoglycan of α1→2-Linkedd-glycero-d-manno-Heptopyranose

Characterization of the O-4 Phosphorylated and O-5 Substituted Kdo Reducing End Group and Sequencing of the Core Oligosaccharide of Aeromonas Salmonicida ssp Salmonicida Lipopolysaccharide Using Tandem Mass Spectrometry

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