Structural investigations of cross-linked hyaluronan

Gamini, Amelia; Paoletti, Sergio; Toffanin, Renato; Micali, Fulvio; Michielin, Luigi; Bevilacqua, C.

doi:10.1016/s0142-9612(01)00231-9

Cited by 25 publications

(15 citation statements)

References 10 publications

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“…Covalently crosslinked HA hydrogels can be formed by means of multiple chemical modifications. [43][44][45][46] HA is degraded by cells through the release of enzymes such as hyaluronidase.…”

Section: Biological Hydrogelsmentioning

confidence: 99%

Hydrogels in Biology and Medicine: From Molecular Principles to Bionanotechnology

et al. 2006

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Hydrophilic polymers are the center of research emphasis in nanotechnology because of their perceived “intelligence”. They can be used as thin films, scaffolds, or nanoparticles in a wide range of biomedical and biological applications. Here we highlight recent developments in engineering uncrosslinked and crosslinked hydrophilic polymers for these applications. Natural, biohybrid, and synthetic hydrophilic polymers and hydrogels are analyzed and their thermodynamic responses are discussed. In addition, examples of the use of hydrogels for various therapeutic applications are given. We show how such systems' intelligent behavior can be used in sensors, microarrays, and imaging. Finally, we outline challenges for the future in integrating hydrogels into biomedical applications.

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Section: Biological Hydrogelsmentioning

confidence: 99%

Hydrogels in Biology and Medicine: From Molecular Principles to Bionanotechnology

et al. 2006

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“…used SAXS to characterize cross-linked hylaronan. Using a scattering profile, I(Q) vs. Q , they determined the polymer junction zone for each gel type (88). SAXS was also used to study how the chemical compositions and concentrations of calcium-alginate gels affected their structures.…”

Section: Small Angle Scattering In Biomaterials Researchmentioning

confidence: 99%

Using small-angle scattering techniques to understand mechanical properties of biopolymer-based biomaterials

Hyland

Taraban

2013

Soft Matter

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The design and engineering of innovative biopolymer-based biomaterials for a variety of biomedical applications should be based on the understanding of the relationship between their nanoscale structure and mechanical properties. Down the road, such understanding could be fundamental to tune the properties of engineered tissues, extracellular matrices for cell delivery and proliferation/differentiation, etc. In this tutorial review, we attempt to show in what way biomaterial structural data can help to understand the bulk material properties. We begin with some background on common types of biopolymers used in biomaterials research, discuss some typical mechanical testing techniques and then review how others in the field of biomaterials have utilized small-angle scattering for material characterization. Detailed examples are then used to show the full range of possible characterization techniques available for biopolymer-based biomaterials. Future developments in the area of material characterization by small-angle scattering will undoubtedly facilitate the use of structural data to control the kinetics of assembly and final properties of prospective biomaterials.

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“…Collagen is a natural ECM containing many cell signaling domains(11) that can be gelled without chemical modifications. (12) HA is a glycosaminoglycan heteropolysaccharide (13, 14) that has been crosslinked to form gels by multiple chemical modification means (15-18), including the methacrylation of HA macromers (MeHA) to produce UV crosslinkable polymer solutions. (19, 20) HA has previously been incorporated into composite hydrogels with synthetic poly(ethylene glycol) components for cellular studies.…”

Section: Introductionmentioning

confidence: 99%

Mechanically Robust and Bioadhesive Collagen and Photocrosslinkable Hyaluronic Acid Semi-Interpenetrating Networks

Brigham

Bick²,

Lo³

et al. 2009

Tissue Engineering Part A

173

149

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In this work, we present a class of hydrogels that leverage the favorable properties of the photocrosslinkable hyaluronic acid (HA) and semi-interpenetrating collagen components. The mechanical properties of the semi-interpenetrating network (semi-IPN) hydrogels far surpass those achievable with collagen gels or collagen gel based semi-IPNs. Furthermore, the inclusion of the semi-interpenetrating collagen chains provides a synergistic mechanical improvement in comparison to unmodified HA hydrogels. Collagen-HA semi-IPNs supported fibroblast adhesion and proliferation and were shown to be suitable for cell encapsulation at high levels of cell viability. To demonstrate the utility of the semi-IPNs as a microscale tissue engineering material, cell laden microstructures and microchannels were fabricated via soft lithographic techniques. Given their enhanced mechanical and biomimetic properties, we anticipate that these materials will be of value in tissue engineering and 3D cell culture applications.

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Structural investigations of cross-linked hyaluronan

Cited by 25 publications

References 10 publications

Hydrogels in Biology and Medicine: From Molecular Principles to Bionanotechnology

Hydrogels in Biology and Medicine: From Molecular Principles to Bionanotechnology

Using small-angle scattering techniques to understand mechanical properties of biopolymer-based biomaterials

Mechanically Robust and Bioadhesive Collagen and Photocrosslinkable Hyaluronic Acid Semi-Interpenetrating Networks

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