Structural messenger RNA contains cytokeratin polymerization and depolymerization signals

Kloc, Małgorzata; Dallaire, Paul; Reunov, A. A.; Major, François

doi:10.1007/s00441-011-1255-x

Cited by 19 publications

(15 citation statements)

References 33 publications

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“…A precedent for an mRNA with dual functions comes from the Xenopus oocyte, where the VegT mRNA both encodes a protein and plays a structural role in organization of the cytokeratin cytoskeleton (Heasman et al 2001;Kloc et al 2005Kloc et al , 2007. Different regions of the VegT mRNA have different effects on cytokeratin polymerization and depolymerization (Kloc et al 2011). Loss of either VegT or osk mRNAs perturbs the subcellular distribution of other molecules-certain localized mRNAs for VegT, and proteins in RNPs for oskbut at present there are no clear parallels in how the osk and VegT mRNAs perform their noncoding roles.…”

Section: Discussionmentioning

confidence: 99%

oskar RNA plays multiple noncoding roles to support oogenesis and maintain integrity of the germline/soma distinction

Kanke

Jambor

Reich

et al. 2015

RNA

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The Drosophila oskar (osk) mRNA is unusual in that it has both coding and noncoding functions. As an mRNA, osk encodes a protein required for embryonic patterning and germ cell formation. Independent of that function, the absence of osk mRNA disrupts formation of the karyosome and blocks progression through oogenesis. Here we show that loss of osk mRNA also affects the distribution of regulatory proteins, relaxing their association with large RNPs within the germline, and allowing them to accumulate in the somatic follicle cells. This and other noncoding functions of the osk mRNA are mediated by multiple sequence elements with distinct roles. One role, provided by numerous binding sites in two distinct regions of the osk 3 ′ UTR, is to sequester the translational regulator Bruno (Bru), which itself controls translation of osk mRNA. This defines a novel regulatory circuit, with Bru restricting the activity of osk, and osk in turn restricting the activity of Bru. Other functional elements, which do not bind Bru and are positioned close to the 3 ′ end of the RNA, act in the oocyte and are essential. Despite the different roles played by the different types of elements contributing to RNA function, mutation of any leads to accumulation of the germline regulatory factors in the follicle cells.

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Section: Discussionmentioning

confidence: 99%

oskar RNA plays multiple noncoding roles to support oogenesis and maintain integrity of the germline/soma distinction

Kanke

Jambor

Reich

et al. 2015

RNA

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“…We focused on a SNP in miR-125a that interferes with maturation and is highly associated with breast cancer patients (36), the ribosomal A-site, and a few other miRNA examples. In general, our model possibly applies to many, if not all, RNAs, and links RNA transition networks and function (45,46). Besides, this model suggests similarities between RNA and intrinsically disordered proteins, which fold and function upon specific chemical signals shown crucial in ligand binding (47).…”

Section: Discussionmentioning

confidence: 99%

Structural dynamics control the MicroRNA maturation pathway

et al. 2016

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MicroRNAs (miRNAs) are crucial gene expression regulators and first-order suspects in the development and progression of many diseases. Comparative analysis of cancer cell expression data highlights many deregulated miRNAs. Low expression of miR-125a was related to poor breast cancer prognosis. Interestingly, a single nucleotide polymorphism (SNP) in miR-125a was located within a minor allele expressed by breast cancer patients. The SNP is not predicted to affect the ground state structure of the primary transcript or precursor, but neither the precursor nor mature product is detected by RT-qPCR. How this SNP modulates the maturation of miR-125a is poorly understood. Here, building upon a model of RNA dynamics derived from nuclear magnetic resonance studies, we developed a quantitative model enabling the visualization and comparison of networks of transient structures. We observed a high correlation between the distances between networks of variants with that of their respective wild types and their relative degrees of maturation to the latter, suggesting an important role of transient structures in miRNA homeostasis. We classified the human miRNAs according to pairwise distances between their networks of transient structures.

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“…Very sadly, in March of 2006, Larry Etkin died at the age of 61. His lab was dissolved, but Malgorzata Kloc found a new position and carried on with these studies at an ultrastructural level (Kloc, Dallaire, Reunov, & Major, ; reviewed in Kloc, Foreman, and Reddy ().…”

Section: Anchoring Localized Mrnas To the Cortex: The Concept Of Binamentioning

confidence: 99%

Maternal messages to live by: a personal historical perspective

King

2017

Genesis

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SUMMARY In the 1980’s, the study of localized maternal mRNAs was just emerging as a new research area. Classic embryological studies had linked the inheritance of cytoplasmic domains with specific cell lineages, but the underlying molecular nature of these putative determinants remained a mystery. The model system Xenopus would play a pivotal role in the progress of this new field. In fact, the first localized maternal mRNA to be identified and cloned from any organism was Xenopus vg1, a TGF-beta family member. This seminal finding opened the door to many subsequent studies focused on how RNAs are localized and what functions they had in development. Xenopus remains the model of choice for studies on dissecting RNA/protein transport particles and RNA-sequencing analyses that are moving the field into the future.

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Structural messenger RNA contains cytokeratin polymerization and depolymerization signals

Cited by 19 publications

References 33 publications

oskar RNA plays multiple noncoding roles to support oogenesis and maintain integrity of the germline/soma distinction

oskar RNA plays multiple noncoding roles to support oogenesis and maintain integrity of the germline/soma distinction

Structural dynamics control the MicroRNA maturation pathway

Maternal messages to live by: a personal historical perspective

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