Structural, physicochemical and functional properties of Semen Ziziphi Spinosae protein

Abstract

We determined and analyzed the structural, physicochemical and functional properties of Semen Ziziphi Spinosae protein. It shows that SZSP has good processing properties and can become a new plant protein source.

Physico‐chemical properties, antioxidant activity, and ACE inhibitory activity of protein hydrolysates from wild jujube seed

“…SDS‐PAGE electrophoresis is commonly used for molecular weight and subunit analysis of protein and hydrolysates (Zhang et al., 2020). Tricine‐SDS‐PAGE can be used to isolate proteins and peptides with molecular weight of 1–10 kDa, and has become the main determination method for molecular weight of hydrolysates and polypeptides (Mundi & Aluko, 2012).…”

“…WJSP was prepared from the wild jujube seeds defatted powder using the methods described by Zhang et al. (2020) with some alterations. Then WJSP was dissolved in deionized water to prepare a 5% protein solution (w/v).…”

“…CD spectroscopy is a commonly used method to analyze the secondary structure changes. In this study, CD spectroscopy of WJSP and WJSPHs was scanned on the basis of the modified method of Zhang et al (2020) on a JASCO J−1100 CD spectrometer (Jasco Corp, Tokyo, Japan) in the far-UV range of 180-260 nm. The measurement parameters were as follow: Scan speed was 100 nm/min, bandwidth was 1.0 nm, response time was 0.50 s, and step resolution was 1.0 nm at 25°C.…”

“…Fourier transforms infrared (FTIR) spectroscopy FTIR spectroscopy of WJSP and WJSPHs were determined on the basis of the method described by Zhang et al (2020). Briefly, FTIR spectroscopy of WJSP and WJSPHs samples were obtained via a Fourier-transform infrared spectrometer (Nicolet 380, Thermo Scientific, UK) in the range of 4,000 and 400 cm −1 at 25 °C.…”

“…Wild jujube seed protein (WJSP) occupies approximately 36% on a dry weight basis of wild jujube seed. Our previous studies found that WJSP can be used as one kind of popular functional food material to promote anti‐inflammatory and immunomodulatory effects (Zhang et al., 2020). However, owing to its large molecular weight and complex structure, biological activities of WJSP, such as antioxidant activity and antifatigue, are greatly limited and cannot be fully utilized by the human body after ingestion.…”