Structural, physicochemical properties and function of swim bladder collagen in promoting fibroblasts viability and collagen synthesis

Xiao, Lanfei; Lv, Jiehuan; Liang, Yongjun; Zhang, Haoran; Zheng, Jia; Lin, Fan; Wen, Xiaobo

doi:10.1016/j.lwt.2022.114294

Cited by 13 publications

(6 citation statements)

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“…Both the collagen displayed a highly porous structure, multi layered and interconnected pores with ASC exhibited more compact structure than PSC. Considering the microstructure, it is clear that the regular network structure could offer a suitable environment for cell proliferation and migration which is vital for wound dressing biomaterial [25].…”

Section: Morphological Analysismentioning

confidence: 99%

Bioconversion of Eel Skin Waste into Valuable Collagen: Isolation, Spectral Characterization, and Biocompatibility Assessment

Asharaf,

S.R.,

Rajan

2024

Waste Biomass Valor

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The current investigation was carried out to evaluate the structural and cell proliferation capability of pike conger eel collagen derived by acid and enzymatic extraction method. The recovery yield of pepsin soluble collagen (PSC) (40.65%) was significantly higher (p<0.05) than acid soluble collagen (ASC) (29.9%) on dry weight basis. In terms of molecular weight pattern, α1 and α2 bands of extracted collagen occurred to be 130KDa and 112KDa and was confirmed as Type 1 structure. The presence of characteristics peaks of amide bands in Fourier Transform Infrared Spectra (FTIR) and the d value of 12.28 Å and 11.37 Å in X-ray diffraction (XRD) revealed no significant changes in the highly ordered intact triple helical structure of collagen through pepsin digestion. The higher thermal stability with denaturation temperature of 33.3°C for ASC and 35.1°C for PSC was well documented in the differential scanning calorimetry (DSC) curve which showed a substantial correlation with the findings of imino acid content of both ASC and PSC collagen (203.5 ± 1.06 and 216.81 ± 0.32 residues/1000g respectively). In addition, the nontoxic behaviour of isolated collagen samples to L929 fibroblast cell lines emphasized the possible utilization of pike conger eel skin as a safe source of thermal stable collagen.

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Section: Morphological Analysismentioning

confidence: 99%

Bioconversion of Eel Skin Waste into Valuable Collagen: Isolation, Spectral Characterization, and Biocompatibility Assessment

Asharaf,

S.R.,

Rajan

2024

Waste Biomass Valor

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“…The biological activities of proteins are closely related to their amino acid distribution and the composition of their R group. 26,27 Amino acid composition of isinglass was determined to understand its influence on antioxidant activity. The presence of aromatic amino acids, such as phenylalanine and tyrosine, contributes to the antioxidant activity of proteins.…”

Section: Amino Acid Composition Of Isinglassmentioning

confidence: 99%

“…The contents of proline and hydroxyproline (15.47%) contributed to the stability of collagen's triple helical structure, but they were lower than that of collagen extracted directly from the swim bladder. 26 Glutamate and aspartic acid are acidic amino acids, whereas lysine, arginine and histidine are basic amino acids. It has been reported that acidic and basic amino acid residues play a key role in chelating metal ions, like iron and copper.…”

Section: Amino Acid Composition Of Isinglassmentioning

confidence: 99%

Identification of antioxidant peptides after digestion and absorption of isinglass by serum peptidomics and cellular antioxidant activity analysis

Xiao

Liang

et al. 2023

Food Funct.

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“…Collagen has been isolated mostly from fish skin and occasionally from swim bladders. The swim bladder of some fish species is a valuable source of bioactive compounds, mainly collagen [ 9 , 10 , 11 ]. There are various reports on preparation methods for collagen extraction from several marine and freshwater fish species and the biochemical characteristics of the extracted collagen [ 12 , 13 , 14 , 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

Swim Bladder of Farmed Totoaba macdonaldi: A Source of Value-Added Collagen

et al. 2023

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Finding strategies to use the swim bladder of farmed totoaba (Totoaba macdonaldi) is of the utmost need to reduce waste. Fish swim bladders are rich in collagen; hence, extracting collagen is a promising alternative with benefits for aquaculture of totoaba and the environment. The elemental biochemical composition of totoaba swim bladders, including their proximate and amino acid compositions, was determined. Pepsin-soluble collagen (PSC) was used to extract collagen from swim bladders, and its characteristics were analyzed. Alcalase and papain were used for the preparation of collagen hydrolysates. Swim bladders contained 95% protein, 2.4% fat, and 0.8% ash (on a dry basis). The essential amino acid content was low, but the functional amino acid content was high. The PSC yield was high, at 68% (dry weight). The amino acid composition profile, electrophoretic pattern, and structural integrity analyses of the isolated collagen suggested it is a typical type-I collagen with high purity. The denaturalization temperature was 32.5 °C, probably attributable to the imino acid content (205 residues/1000 residues). Papain-hydrolysates (≤3 kDa) of this collagen exhibited higher radical scavenging activity than Alcalase-hydrolysates. The swim bladder from the farmed totoaba could be an ideal source to produce high-quality type I collagen and may be considered an alternative to conventional collagen sources or bioactive peptides.

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Structural, physicochemical properties and function of swim bladder collagen in promoting fibroblasts viability and collagen synthesis

Cited by 13 publications

References 50 publications

Bioconversion of Eel Skin Waste into Valuable Collagen: Isolation, Spectral Characterization, and Biocompatibility Assessment

Bioconversion of Eel Skin Waste into Valuable Collagen: Isolation, Spectral Characterization, and Biocompatibility Assessment

Identification of antioxidant peptides after digestion and absorption of isinglass by serum peptidomics and cellular antioxidant activity analysis

Swim Bladder of Farmed Totoaba macdonaldi: A Source of Value-Added Collagen

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