Structural plasticity of identified glomeruli in the antennal lobes of the adult worker honey bee

Winnington, Andrew; Napper, Ruth M. A.; Mercer, Alison R.

doi:10.1002/(sici)1096-9861(19960212)365:3<479::aid-cne10>3.0.co;2-m

Cited by 106 publications

(92 citation statements)

References 71 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Perhaps it is because workers spend their younger days in the well-protected and environmentally stable core of the hive. The rising curve also matches the age-dependent development of antenna sensitivity (Robinson, 1987), the volume increase of the glomerular neuropile (Winnington et al, 1996), and the development of mitochondria in flight muscles (unpublished data). This pattern may reflect the change in blood JH titer, which also rises with age (Sasagawa and Kuwahara, 1988;Robinson, 1992).…”

Section: Ontogenic Development Of Learning Capability In Worker and Dsupporting

confidence: 60%

“…So, when and how is the learning ability acquired-is it developed endogenously or it is developed in response to sensory inputs? Coss et al (1980) noted that the dendritic spines in the calycal interneurons in the mushroom body are different between young and old foragers, and recent works (Gascuel and Masson, 1987;Withers et al, 1993Withers et al, , 1995Durst et al, 1994;Farhbach and Robinson, 1996;Winnington et al, 1996;Fahrbach et al, 1997;Sigg et al, 1997;Morgan et al, 1998) noted the change in the volume of the mushroom body depending on age and foraging experience. However, the relationship between flight experience and such morphological change is still unclear.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Importance of social stimuli for the development of learning capability in honeybees

Ichikawa

Sasaki

2003

Appl. Entomol. Zool.

View full text Add to dashboard Cite

The learning ability of the European honeybee, Apis mellifera, is well known. However, in a proboscis-extension reflex (PER) assay, newly emerged and very young worker bees could not associate a given odor (conditioned stimulus, CS) with a sucrose reward (unconditioned stimulus, US): This ability was acquired 5 to 9 days after emergence in workers, while it was accomplished 2 to 5 days after emergence in drones, probably reflecting the earlier onset of flight in drones. When workers are reared individually in a confined condition deprived of colony odor and other social stimuli, they do not develop the ability even after 9 days after emergence. In a series of experiments subjecting the bees to the confined condition for various lengths and timings, the important period for acquiring the learning ability was from day 2 to 6 after emergence. However, even bees that acquired the ability lost it when exposed to the confined (stimuli-deprived) condition for the next 15 days, meaning that the continuous input of appropriate sensory stimuli is essential for both acquiring and maintaining the learning capability.

show abstract

Section: Ontogenic Development Of Learning Capability In Worker and Dsupporting

confidence: 60%

Section: Introductionmentioning

confidence: 99%

Importance of social stimuli for the development of learning capability in honeybees

Ichikawa

Sasaki

2003

Appl. Entomol. Zool.

View full text Add to dashboard Cite

show abstract

“…Daly et al (2004) have recently shown that olfactory conditioning is combined with a restructuring of electrical odor response patterns in the AL. Work on adult neuronal plasticity in the honeybee has demonstrated that increases in adult glomerular volume are activity-dependent and correlated with better learning performance (Winnington et al 1996;Sigg et al 1997). Quantitative electron-microscopic studies have lead to the suggestion that volume increase and synapse number changes are independent processes that both contribute to structural plasticity in the AL, although synapse reorganization might only play a minor role on neuropilar volume (Brown et al 2002).…”

Section: Discussionmentioning

confidence: 99%

Standard three-dimensional glomeruli of the Manduca sexta antennal lobe: a tool to study both developmental and adult neuronal plasticity

Huetteroth

Schachtner

2005

Cell Tissue Res

View full text Add to dashboard Cite

The metamorphosing antennal lobe (AL) of the sphinx moth Manduca sexta serves as an established model system for studying neuronal development. To improve our understanding of mechanisms involved in neuronal plasticity, we have analyzed the size, shape, and localization of ten identified glomeruli at three different time points during development and in the adult, viz., (1) 13 days after pupal eclosion (P13), which reflects a time when the basic glomerular map has formed, (2) immediately after adult eclosion (A0), which represents a time when the newly formed glomeruli are uninfluenced by external odors, and (3) 4 days after adult eclosion (A4), which reflects a time when the animals have been exposed to surrounding odors. Our data from normally developing ALs of male M. sexta from P13 to A0 revealed an increase in size of all examined glomeruli of between 40% and 130%, with the strongest increases occurring in two of the three sex-specific glomeruli (cumulus, toroid). From A0 to A4, the cumulus and toroid increased significantly when correlated to AL volume, whereas the other glomeruli reached the sizes gained after A0. This study was based on antibody staining against the ubiquitous synaptic vesicle protein synaptotagmin, confocal laser scan microscopy, and the three-dimensional (3D) analysis tool AMIRA. Tissue permeability and therefore reliability of the staining quality was enhanced by using formalin/methanol fixation. The standard 3D glomeruli introduced in this study can now be used as basic tools for further examination of neuronal plasticity at the level of the identified neuropil structures, viz., the glomeruli of the AL of M. sexta.

show abstract

“…Olfactory glomeruli are plastic structures undergoing volume changes even during adulthood, depending on previous experience (Drosophila: Devaud et al [2001, 2003; Sachse et al [2007]; Apis : Winnington et al [1996]; Sigg et al [1997]). The latter studies showed significant volume changes in glomeruli after foraging experience, although the bees' actual activity during foraging was not controlled.…”

Section: Changes In Glomerular Volume As a Possible Memory Tracementioning

confidence: 99%

Long-term memory shapes the primary olfactory center of an insect brain

Hourcade¹,

Perisse²,

Devaud³

et al. 2009

Learn. Mem.

View full text Add to dashboard Cite

The storage of stable memories is generally considered to rely on changes in the functional properties and/or the synaptic connectivity of neural networks. However, these changes are not easily tractable given the complexity of the learning procedures and brain circuits studied. Such a search can be narrowed down by studying memories of specific stimuli in a given sensory modality and by working on networks with a modular and relatively simple organization. We have therefore focused on associative memories of individual odors and the possible related changes in the honeybee primary olfactory center, the antennal lobe (AL). As this brain structure is organized in well-identified morpho-functional units, the glomeruli, we looked for evidence of structural and functional plasticity in these units in relation with the bees' ability to store long-term memories (LTMs) of specific odors. Restrained bees were trained to form an odor-specific LTM in an appetitive Pavlovian conditioning protocol. The stability and specificity of this memory was tested behaviorally 3 d after conditioning. At that time, we performed both a structural and a functional analysis on a subset of 17 identified glomeruli by measuring glomerular volume under confocal microscopy, and odor-evoked activity, using in vivo calcium imaging. We show that long-term olfactory memory for a given odor is associated with volume increases in a subset of glomeruli. Independent of these structural changes, odor-evoked activity was not modified. Lastly, we show that structural glomerular plasticity can be predicted based on a putative model of interglomerular connections.[Supplemental material is available online at http: //www.learnmem.org.]In nature, animals' survival relies on their capacity to adapt to changes in the environment by constantly learning and memorizing novel information and modifying their behavior accordingly. This capacity relies on the long-term storage of learned information involving specific stable modifications of neural networks, both in their connectivity and in the strength of synaptic transmission (Matsuzaki 2007). For such long-term memory (LTM) to be specific for particular stimuli (for instance, an odor), they must rely on specific neural traces of these stimuli in the brain. A crucial question is whether such specific traces can be tracked down to individual neural units, given the complexity of the neuronal networks usually involved in the formation of LTM. Brain regions constituted of relatively few neurons and organized in clearly identified modules offer an excellent opportunity to answer this question. Among these is the insect antennal lobe (AL), an olfactory center that shares many similarities with the vertebrate olfactory bulb, but provides numerical simplicity. In the honeybee, the AL contains ;160 interconnected neuropil subunits, the glomeruli, which can be unambiguously identified across individuals (Galizia et al. 1999a). Experiments using in vivo calcium imaging have shown that glomeruli are functional units for odor...

show abstract

Structural plasticity of identified glomeruli in the antennal lobes of the adult worker honey bee

Cited by 106 publications

References 71 publications

Importance of social stimuli for the development of learning capability in honeybees

Importance of social stimuli for the development of learning capability in honeybees

Standard three-dimensional glomeruli of the Manduca sexta antennal lobe: a tool to study both developmental and adult neuronal plasticity

Long-term memory shapes the primary olfactory center of an insect brain

Contact Info

Product

Resources

About