Structural proteins of kilham rat virus

Characterization of newly isolated rat viruses from asymptomatic laboratory rats

“…Antiserum against RV-13 similarly detect VP-1 (81k), VP-2 (61k) and VP-3 (59k) separated by SDS-PAGE in UT-1 (1), UT-2 (2), and RV-13 (3) PAGE, we confirmed that each strain had three structural polypeptides. VP-1, VP-2, and VP-3, as described by Salzman and White [8]. The molecular ratios of these proteins were estimated to be 81 K, 61 K, and 59 K, respectively, and then all reacted similarly with the respective immune sera against UT-1, UT-2, and RV-13 in western blotting (Fig.…”

Comparative Physicochemical and Biological Properties of Two Strains of Kilham Rat Virus, a Non-defective Parvovirus

“…Fig. 2 shows that the three major proteins described by Peterson et al (1978) and Salzman & White (1970) for KRV308 and designated A, B and C, are present in both the mature virions and the empty capsids of both strains. The moL wt.…”

“…Normal rat kidney (NRK) (Duc-Nguyen et al, 1966) and rat nephroma (Salzman & Jori, 1970) cells were routinely grown in Dulbecco's modified minimal essential medium containing 10 % foetal calf serum. Plaque-purified KRV308 (clone IA) was a gift of L. Salzman (NIH, Bethesda, Md., U.S.A.) and was used without further purification.…”

“…Host range of KR V strains KRV171 has been routinely grown in primary rat embryo or NRK cells (Tennant & Hand, 1970;Lavelle & Li, 1977), and KRV308 has been grown in rat nephroma cells (Salzman & Jori, 1970). Earlier observations that KRV 171 did not grow in rat nephroma cells led to the suspicion that KRV171 and KRV308, despite their antigenic similarity, are not identical Fig.…”

“…Both strains have been extensively studied as prototypes of KRV (Tennant & Hand, 1970;Salzman & Jori, 1970;Astell et al, 1979;LaveUe & Mitra, 1978). However, the observation "~ Present address: Department of Microbiology, University of Alabama at Birmingham, Birmingham, Alabama 35294, U.S.A. of R. Hand & R. Tennant (personal communication) that KRV171 does not infect rat nephroma cells, commonly used as host for KRV308 (Salzman & Jori, 1970), suggested a difference in one or more of their structural properties. In this report, we document some of the distinct physicochemical properties of the two KRV strains.…”