Structural region essential for amyloid fibril formation in cytochrome <i>c</i> elucidated by optical trapping

Hirota, Shun; Chiu, Chun-Liang; Chang, Chieh-Ju; Lo, Pei-Hua; Chen, Tien; Yang, Huiting; Yamanaka, Masahito; Mashima, Tsuyoshi; Xie, Cheng; Masuhara, Hiroshi; Sugiyama, Teruki

doi:10.1039/d2cc04647d

Cited by 3 publications

(1 citation statement)

References 37 publications

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“…7,8 Multiple technologies have been developed for the smart detection of Cyt c using interferometric reflectance spectroscopy (IRS), 9 surface-enhanced Raman scattering (SERS), 10 electrochemiluminescence, 11 fluorescence immunoassay, 12,13 surface assisted laser desorption/ ionization mass spectrometry (SALDI-MS), 14 electrochemical immunoassay, 15,16 and others. 17 For example, Zhang et al developed an unmarked nitrogen-doped carbon dot nanosensor for quantitative measurement of Cyt c release in cell apoptosis via fluorescence activation imaging. 12 Tan et al created a molecularly imprinted polymer sensor by hybridizing aptamer molecularly imprinted polymers with quantum dots.…”

Section: Introductionmentioning

confidence: 99%

Portable self-powered photoelectrochemical immunosensor based on Cu₃SnS₄ nanoflower for ultra-sensitive and real-time detection of human cytochrome c

Zhang¹,

Jin²,

Xiao³

et al. 2023

Inorg. Chem. Front.

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Section: Introductionmentioning

confidence: 99%

Portable self-powered photoelectrochemical immunosensor based on Cu₃SnS₄ nanoflower for ultra-sensitive and real-time detection of human cytochrome c

Zhang¹,

Jin²,

Xiao³

et al. 2023

Inorg. Chem. Front.

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Optical trapping-induced crystallization promoted by gold and silicon nanoparticles

Su,

Liu,

Fujii

et al. 2024

Photochem Photobiol Sci

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This study investigates the promotion of sodium chlorate (NaClO3) crystallization through optical trapping, enhanced by the addition of gold nanoparticles (AuNPs) and silicon nanoparticles (SiNPs). Using a focused laser beam at the air–solution interface of a saturated NaClO3 solution with AuNPs or SiNPs, the aggregates of these particles were formed at the laser focus, the nucleation and growth of metastable NaClO3 (m-NaClO3) crystals were induced. Continued laser irradiation caused these m-NaClO3 crystals to undergo repeated cycles of growth and dissolution, eventually transitioning to a stable crystal form. Our comparative analysis showed that AuNPs, due to their significant heating due to higher photon absorption efficiency, caused more pronounced size fluctuations in m-NaClO3 crystals compared to the stable behavior observed with SiNPs. Interestingly, the maximum diameter of the m-NaClO3 crystals that appeared during the size fluctuation step was consistent, regardless of nanoparticle type, concentration, or size. The crystallization process was also promoted by using polystyrene nanoparticles, which have minimal heating and electric field enhancement, suggesting that the reduction in activation energy for nucleation at the particle surface is a key factor. These findings provide critical insights into the mechanisms of laser-induced crystallization, emphasizing the roles of plasmonic heating, particle surfaces, and optical forces. Graphical abstract

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Experimental and Computational Studies on Domain-Swapped Structure Stabilization of an Antibody Light Chain by Disulfide Bond Introduction

Fitriana,

Sakai,

Duan

et al. 2024

J. Med. Chem.

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Development of different platforms would be useful for designing functional antibodies to improve the efficiency of antibody-based drugs. Threedimensional domain swapping (3D-DS) may occur in the variable region of antibody light chain #4C214A, and a pair of domain-swapped dimers may interact with each other to form a tetramer. In this study, to stabilize the 3D-DS dimer structure in #4C214A, Val2 in strand A (swapping region) and Thr97 in strand G were replaced with Cys residues, generating #4 V2C/T97C/C214A with a Cys2− Cys97 disulfide bond that cross-links strands A and G of different protomers. The #4 V2C/T97C/C214A tetramer did not dissociate into monomers at low protein concentration (6 μM); however, some of the tetramers were converted to monomers by disulfide bond reduction. Two-dimensional free energy profile analysis for the tetramerization of two 3D-DS dimers was performed by molecular dynamics simulation. These results show that disulfide bond introduction is useful for controlling the dimerization/dissociation of the variable region through 3D-DS.

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Structural region essential for amyloid fibril formation in cytochrome c elucidated by optical trapping

Abstract: Amyloid fibril formation of cytochrome c is spatially and temporally controlled by the optical trapping method, identifying that the structural change in the region containing Ala83 is essential for the amyloid fibril formation.

Cited by 3 publications

References 37 publications

Portable self-powered photoelectrochemical immunosensor based on Cu₃SnS₄ nanoflower for ultra-sensitive and real-time detection of human cytochrome c

Portable self-powered photoelectrochemical immunosensor based on Cu₃SnS₄ nanoflower for ultra-sensitive and real-time detection of human cytochrome c

Optical trapping-induced crystallization promoted by gold and silicon nanoparticles

Experimental and Computational Studies on Domain-Swapped Structure Stabilization of an Antibody Light Chain by Disulfide Bond Introduction

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Structural region essential for amyloid fibril formation in cytochrome c elucidated by optical trapping

Abstract: Amyloid fibril formation of cytochrome c is spatially and temporally controlled by the optical trapping method, identifying that the structural change in the region containing Ala83 is essential for the amyloid fibril formation.

Cited by 3 publications

References 37 publications

Portable self-powered photoelectrochemical immunosensor based on Cu3SnS4 nanoflower for ultra-sensitive and real-time detection of human cytochrome c

Portable self-powered photoelectrochemical immunosensor based on Cu3SnS4 nanoflower for ultra-sensitive and real-time detection of human cytochrome c

Optical trapping-induced crystallization promoted by gold and silicon nanoparticles

Experimental and Computational Studies on Domain-Swapped Structure Stabilization of an Antibody Light Chain by Disulfide Bond Introduction

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Product

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Portable self-powered photoelectrochemical immunosensor based on Cu₃SnS₄ nanoflower for ultra-sensitive and real-time detection of human cytochrome c

Portable self-powered photoelectrochemical immunosensor based on Cu₃SnS₄ nanoflower for ultra-sensitive and real-time detection of human cytochrome c