Structural relationship between the nucleolus and the nuclear envelope

Bourgeois, Claire A.; Hémon, Denis; Bouteille, M.

doi:10.1016/s0022-5320(79)90165-5

Cited by 77 publications

(43 citation statements)

References 25 publications

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“…6B, bottom image). In human cells it has long been reported that there are connections between the nuclear envelope and the nucleolus (Bourgeois et al 1979); here, however, we found no evidence of such contacts. This observation is more apparent in cells in which the lamina was marked by immunostaining and the nucleolus with S9-GFP: There is no evidence by confocal microscopy of either a contact with the nucleolus or nuclear envelope invaginations (see Supplemental Fig.…”

Section: Development Of Transgenic Drosophila Which Allows Visualizatcontrasting

confidence: 93%

Visualization of the joining of ribosomal subunits reveals the presence of 80S ribosomes in the nucleus

Al-Jubran

Wen

Abdullahi

et al. 2013

RNA

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In eukaryotes the 40S and 60S ribosomal subunits are assembled in the nucleolus, but there appear to be mechanisms preventing mRNA binding, 80S formation, and initiation of translation in the nucleus. To visualize association between ribosomal subunits, we tagged pairs of Drosophila ribosomal proteins (RPs) located in different subunits with mutually complementing halves of fluorescent proteins. Pairs of tagged RPs expected to interact, or be adjacent in the 80S structure, showed strong fluorescence, while pairs that were not in close proximity did not. Moreover, the complementation signal is found in ribosomal fractions and it was enhanced by translation elongation inhibitors and reduced by initiation inhibitors. Our technique achieved 80S visualization both in cultured cells and in fly tissues in vivo. Notably, while the main 80S signal was in the cytoplasm, clear signals were also seen in the nucleolus and at other nuclear sites. Furthermore, we detected rapid puromycin incorporation in the nucleolus and at transcription sites, providing an independent indication of functional 80S in the nucleolus and 80S association with nascent transcripts.

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Section: Development Of Transgenic Drosophila Which Allows Visualizatcontrasting

confidence: 93%

Visualization of the joining of ribosomal subunits reveals the presence of 80S ribosomes in the nucleus

Al-Jubran

Wen

Abdullahi

et al. 2013

RNA

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“…The nuclear foci of BRCA1 protein are widely distributed within the nucleoplasm, but they are excluded from nucleoli. The termination of a subset of invaginations of the nuclear envelope adjacent to nucleoli (Bourgeois et al, 1979;Fricker et al, 1997) together with exclusion of BRCA1 foci from within nucleoli may account for a previous interpretation that BRCA1 foci associated with invaginations of the nuclear envelope (Coene et al, 1997). However, at the ultrastructural level, it is clear that the BRCA1 foci do not show obvious association with the nuclear envelope, either at the nuclear margin or on the walls of nuclear invaginations.…”

Section: Discussionmentioning

confidence: 85%

Phosphorylated BRCA1 Is Predominantly Located in the Nucleus and Mitochondria

Coene

Hollinshead

Waeytens

et al. 2005

MBoC

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Multiple copies of the mitochondrial genome in eukaryotic cells are organized into protein-DNA complexes called nucleoids. Mitochondrial genome repair mechanisms have been reported, but they are less well characterized than their nuclear counterparts. To expand our knowledge of mitochondrial genome maintenance, we have studied the localization of the BRCA1 protein, known to be involved in nuclear repair pathways. Our confocal and immunoelectron microscopy results show that BRCA1 is present in mitochondria of several human cancer cell lines and in primary breast and nasal epithelial cells. BRCA1 localization in mitochondria frequently overlapped that of nucleoids. Small interfering RNAmediated knockdown of BRCA1 in human cancer cells (confirmed by Western blot) results in decreased nuclear, cytoplasmic, and mitochondrial staining after immunofluorescence microscopy, establishing the specificity of the BRCA1 immunolabeling. Furthermore, using cell fractionation, dephosphorylation, and enzyme protection experiments, we show that a 220-kDa phosphorylated isoform of BRCA1 is enriched in mitochondrial and nuclear fractions but reduced in cytoplasmic subcellular fractions. Submitochondrial fractionation confirmed the presence of BRCA1 protein in isolated mitoplasts. Because phosphorylation of BRCA1 and subsequent changes in subcellular localization are known to follow DNA damage, our data support a universal role for BRCA1 in the maintenance of genome integrity in both mitochondria and nucleus.

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“…Such approaches allow the identification of dynamic tubular channels which appear as deep, narrow invaginations of the nuclear envelope. These invaginations, or infoldings, first described in 1979 (Bourgeois, et al, 1979), have been observed in a lot of cell types (Clubb and Locke, 1998, Collado-Hilly, et al, 2010, Fricker, et al, 1997, Johnson, et al, 2003, Langevin, et al, 2010, Lui, et al, 2003, Wittmann, et al, 2009. The invaginations of the NE have been recently classified into two main classes depending on whether the ONM is involved (Malhas, et al, 2011).…”

Section: Structure Of the Nuclear Envelopementioning

confidence: 99%

Role of the nuclear envelope in calcium signalling

Mauger

2011

Biology of the Cell

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SummaryThe endoplasmic reticulum is the major Ca 2+ store inside the cell. Its organisation in specialised sub-domains allows the local delivery of Ca 2+ to specific cell areas on stimulation. The nuclear envelope, which is continuous with the endoplasmic reticulum, has a double role: it insulates the nucleoplasm from the cytoplasm and it stores Ca 2+ around the nucleus. Furthermore, all the constituents of the signalling cascade leading to Ca 2+ mobilisation are found in the nuclear envelope; this allows the nuclear Ca 2+ to be regulated autonomously. On the other hand, cytosolic Ca 2+ transients can propagate within the nucleus via the nuclear pore complex. The variations in nuclear Ca 2+ concentration are important for controlling gene transcription and for progression in the cell cycle. Recent data suggest that invaginations of the nuclear envelope modify the morphology of the nucleus and may affect Ca 2+ dynamics in the nucleus and regulate transcriptional activity.

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Structural relationship between the nucleolus and the nuclear envelope

Cited by 77 publications

References 25 publications

Visualization of the joining of ribosomal subunits reveals the presence of 80S ribosomes in the nucleus

Visualization of the joining of ribosomal subunits reveals the presence of 80S ribosomes in the nucleus

Phosphorylated BRCA1 Is Predominantly Located in the Nucleus and Mitochondria

Role of the nuclear envelope in calcium signalling

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